During the car production process, diverse production workshops have distinct prerequisites for car body sequencing. This results in the intricate nature of sequencing within multi-stage car workshops. In this study, an optimization method for car body sequencing is proposed that combines a hybrid evolutionary algorithm with heuristic rules. In the welding workshop, a genetic algorithm is employed to optimize the vehicle sequencing. Simultaneously, a differential evolution algorithm is used to optimize the inbound sequence of the buffer zone between the welding and painting workshops, as well as the inbound sequence of the buffer zone between the painting and assembly workshops. Heuristic rules are applied to optimize the outbound sequence of the buffer zone between the welding and painting workshops, as well as the outbound sequence of the buffer zone between the painting and assembly workshops. In addition, in order to improve the quality of the initial population, a heuristic method-based initial population construction method is proposed. The optimization objectives are the number of vehicle model changes in the welding workshop, the number of color changes in the painting workshop, and the total number of overloads in the assembly workshop. The experimental results show that the proposed method performs better than the five outstanding evolutionary algorithms.