An asymptotic expression of the orthonormal polynomials P N (z) as N → ∞, associated with the singularly perturbed Laguerre weight w α (x; t) = x α e −x− t x , x ∈ [0, ∞), α > −1, t ≥ 0 is derived. Based on this, we establish the asymptotic behavior of the smallest eigenvalue, λ N , of the Hankel matrix generated by the weight w α (x; t).