Smart applications of bionanosensors for BCR/ABL fusion gene detection in leukemia

Avelino, Karen Y.P.S.; Silva, Rafael R.; Silva, Alberto G. da; Oliveira, Maria D.L.; Andrade, César A.S.

doi:10.1016/j.jksus.2017.08.002

Cited by 13 publications

(5 citation statements)

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“…Technological advancements are rapidly changing the face of science in terms of data acquisition, its transfer, storage, analysis, interpretation and dissemination of results [1]. In biology and genomics, this is affecting many traditionally considered purely wet lab experiments like genome sequencing [2], medical diagnosis [3], and drug design [4]. It therefore becomes essential for bioinformaticians to remain up to date with recent trends and innovations in the field.…”

Section: Introductionmentioning

confidence: 99%

Ten simple rules for organizing a webinar series

et al. 2019

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Section: Introductionmentioning

confidence: 99%

Ten simple rules for organizing a webinar series

et al. 2019

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“…This translocation gives rise to chimeric fusion proteins, collectively named BCR-ABL1, whose constitutively activated tyrosine kinase activity is ultimately responsible for the altered differentiation, uncontrolled replication, and resistance to apoptosis typically displayed by CML cells. Most CML patients present a BCR-ABL1 fusion downstream of exons 13 and 14 of the BCR gene, originating transcripts with an e14 and/or an e13 junction, which drive the expression of a 210 kDa chimeric protein designated p210 BCR-ABL1 (M-BCR), although other isoform fusion proteins (namely p190 and p230) have also been described at lower frequencies in some patient subtypes [ 2 , 3 , 4 , 5 , 6 ]. The enduring proliferation of CML stem cells expressing BCR-ABL1 fusion proteins eventually potentiates the occurrence of additional mutations, which are often associated with a more negative prognosis and the eventual development of resistance to clinical treatments [ 7 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

Critical Requirement of SOS1 for Development of BCR/ABL-Driven Chronic Myelogenous Leukemia

Gómez

García-Navas

Baltanás

et al. 2022

Cancers

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We showed previously that the ABL-mediated phosphorylation of SOS1 promotes RAC activation and contributes to BCR-ABL leukemogenesis, suggesting the relevant role of SOS1 in the pathogenesis of CML. To try and obtain direct experimental evidence of the specific mechanistic implication of SOS1 in CML development, here, we combined a murine model of CML driven by a p210BCR/ABL transgene with our tamoxifen-inducible SOS1/2-KO system in order to investigate the phenotypic impact of the direct genetic ablation of SOS1 or SOS2 on the pathogenesis of CML. Our observations showed that, in contrast to control animals expressing normal levels of SOS1 and SOS2 or to single SOS2-KO mice, p210BCR/ABL transgenic mice devoid of SOS1 presented significantly extended survival curves and also displayed an almost complete disappearance of the typical hematological alterations and splenomegaly constituting the hallmarks of CML. SOS1 ablation also resulted in a specific reduction in the proliferation and the total number of colony-forming units arising from the population of bone marrow stem/progenitor cells from p210BCR/ABL transgenic mice. The specific blockade of CML development caused by SOS1 ablation in p210BCR/ABL mice indicates that SOS1 is critically required for CML pathogenesis and supports the consideration of this cellular GEF as a novel, alternative bona fide therapeutic target for CML treatment in the clinic.

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“…Voltammetry is an analytical method that gives us information about the "analyte through electrical currents obtained from a potential variation" [1]. This was first brought about in the 1920s by an experiment conducted by Heyrovský, which measured the surface tension of mercury to provide the information about the nature of the liquid-metal interface.…”

Section: Introductionmentioning

confidence: 99%