Smart Biomimetic Nanocomposites Mediate Mitochondrial Outcome through Aerobic Glycolysis Reprogramming: A Promising Treatment for Lymphoma

Zhao, Qiangqiang; Li, Jian; Wu, Bin; Shang, Yilun; Huang, Xueyuan; Dong, Hang; Liu, Haiting; Chen, Wansong; Gui, Rong; Nie, Xinmin

doi:10.1021/acsami.0c05763

Cited by 37 publications

(15 citation statements)

References 66 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Bcl-2 protein plays an essential role in the regulation of apoptosis (widely expressed in most NHL [ 11 ]). It is mostly located in the mitochondrial intima of cells and inhibits apoptosis by regulating the expression of Bax protein [ 35 ]. As presented in Additional file 1 : Figure S9, Nm@MSNs-DOX/SM can significantly inhibit the expression of Bcl-2 protein and then promote the expression of Bax protein, contributing to inhibiting the apoptosis of SU-DHL-2 cells.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Biomimetic nanotherapy: core–shell structured nanocomplexes based on the neutrophil membrane for targeted therapy of lymphoma

et al. 2021

Self Cite

View full text Add to dashboard Cite

Background Non-Hodgkin’s lymphoma (NHL) is a malignant disease of lymphoid tissue. At present, chemotherapy is still the main method for the treatment of NHL. R-CHOP can significantly improve the survival rate of patients. Unfortunately, DOX is the main cytotoxic drug in R-CHOP and it can lead to adverse reactions. Therefore, it is particularly important to uncover new treatment options for NHL. Results In this study, a novel anti-tumor nanoparticle complex Nm@MSNs-DOX/SM was designed and constructed in this study. Mesoporous silica nanoparticles (MSNs) loaded with Doxorubicin (DOX) and anti-inflammatory drugs Shanzhiside methylester (SM) were used as the core of nanoparticles. Neutrophil membrane (Nm) can be coated with multiple nanonuclei as a shell. DOX combined with SM can enhance the anti-tumor effect, and induce apoptosis of lymphoma cells and inhibit the expression of inflammatory factors related to tumorigenesis depending on the regulation of Bcl-2 family-mediated mitochondrial pathways, such as TNF-α and IL-1β. Consequently, the tumor microenvironment (TME) was reshaped, and the anti-tumor effect of DOX was amplified. Besides, Nm has good biocompatibility and can enhance the EPR effect of Nm@MSNs-DOX/SM and increase the effect of active targeting tumors. Conclusions This suggests that the Nm-modified drug delivery system Nm@MSNs-DOX/SM is a promising targeted chemotherapy and anti-inflammatory therapy nanocomplex, and may be employed as a specific and efficient anti-Lymphoma therapy.

show abstract

Section: Resultsmentioning

confidence: 99%

“…The remaining fluid after dialysis was used to determine the concentration of DOX and SM. Then, the encapsulation efficiency (EE) and drug load efficiency (LE) of two drugs were calculated with the previous method [ 35 ].…”

Section: Methodsmentioning

confidence: 99%

Biomimetic nanotherapy: core–shell structured nanocomplexes based on the neutrophil membrane for targeted therapy of lymphoma

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…After the solution was collected and centrifugated at (10,000 rpm × 2 min), the precipitates were washed with ddH 2 O 3 times to synthesize BPQDs@JB. The encapsulation efficiency (EE) and load efficiency (LE) of JB were calculated (Zhao et al, 2020 ) using EE% = Total mass of JB- mass of JB in supernatant/Total mass of JB × 100%; LE% = Total mass of JB-total mass of JB in supernatant/(Total mass of JB-total mass of JB in supernatant) + mass of BPQDs × 100%.…”

Section: Methodsmentioning

confidence: 99%

A Nano-Traditional Chinese Medicine Against Lymphoma That Regulates the Level of Reactive Oxygen Species

Zhao

et al. 2020

Front. Chem.

Self Cite

View full text Add to dashboard Cite

Jolkinolide B (JB) is a bioactive compound isolated from a Chinese herbal medicine that exerts antitumor activity. However, the anti-lymphoma effect of JB and its mechanism are yet to be revealed. Because free JB has poor pharmacokinetics and weak antitumor efficacy, we opted to use black phosphorus quantum dot (BPQD) nanomaterials as a drug loading platform to synthesize a nano-traditional Chinese medicine (nano-TCM) called BPQDs@JB. Compared with free JB, Raji cells administrated with BPQDs@JB exhibited the cell viability of 19.85 ± 1.02%, and the production of intracellular reactive oxygen species (ROS) was promoted. Likewise, BPQDs@JB was capable of rising the apoptosis rate of Raji cells to 34.98 ± 1.76%. In nude mice transplanted tumor model administrated with BPQDs@JB, the tumor tissue sections administrated with BPQDS@JB achieved a conspicuous red fluorescence, demonstrating the presence of most ROS production in the BPQDS@JB. TUNEL achieved a number of positive (brown) nuclei in vivo, revealing that BPQDS@JB could significantly induce tumor tissue apoptosis. As revealed from the mentioned results, BPQDs@JB can generate considerable ROS and interfere with the redox state to inhibit tumor. In brief, BPQDs@JB may be adopted as a treatment option for lymphoma.

show abstract

“…The entrapment efficiency (EE) and drug loading rate (LE) of HM were calculated. EE was calculated as follows: EE% = total mass of HM − mass of HM in supernatant/total mass of HM × 100%; LE was calculated as follows: LE% = total mass of HM − mass of HM in supernatant/(total mass of HM − mass of HM in supernatant) + mass of MSNs × 100% (Zhao et al 2020).…”

Section: Preparation Of Hm@msnsmentioning

confidence: 99%

Development of a nano-drug delivery system based on mesoporous silica and its anti-lymphoma activity

Zhao

Shang

et al. 2020

Appl Nanosci

Self Cite

View full text Add to dashboard Cite

Although current treatment strategies including systemic chemotherapy, local radiotherapy, biological immunology, and surgical resection have improved the remission rate of non-Hodgkin's lymphoma (NHL) patients, the recurrence rate of the disease remains high, and the remission rate of retreatment after recurrence is low. Exploring and discovering new strategies for treating NHL has become a critical problem to be solved. Harmine (HM) has demonstrated anti-tumor activity; however, like most traditional Chinese medicines, it does not exhibit targeting behavior and is characterized by poor pharmacokinetics, which limits its clinical application. To solve these problems, we constructed a new anti-lymphoma drug delivery system using mesoporous silica nanoparticles (MSNs) loaded with HM. This complex passively targets the tumor tissue, shows pH-responsive drug release, is non-toxic, and has efficient anti-lymphoma properties. Compared with free HM, the tumor inhibition rates of the HM@MSNs was 72.36 ± 5.16% and inhibition of the formation of lymphoma cell clones. Likewise, HM@MSNs could up-regulate the apoptosis rate of Daudi cells to 23.61 ± 0.88%. Moreover, it can inhibit the activation of NF-κB and production of inflammatory cytokines TNF-α (20.9 ± 1.8%) and IL-6 (22.7 ± 2.1%). In nude mice transplanted tumor model treated with HM@MSNs, TUNEL exhibited diffuse red fluorescence in vivo, suggesting that HM@MSNs could significantly induce tumor tissue apoptosis, and HM@MSNs-treated tumor tissue significantly decreased the fluorescence signals of NF-κB p65, TNF-α and IL-6, indicating that it down-regulated the expression of the mentioned three factors, and HM@MSNs significantly inhibited the proliferation of tumor volume. The mentioned results suggested that HM@MSNs finally induced apoptosis of lymphoma cells via anti-inflammatory mechanism. In general, MSNs loaded with HM can be potentially applied to the treatment of NHL.

show abstract

Smart Biomimetic Nanocomposites Mediate Mitochondrial Outcome through Aerobic Glycolysis Reprogramming: A Promising Treatment for Lymphoma

Cited by 37 publications

References 66 publications

Biomimetic nanotherapy: core–shell structured nanocomplexes based on the neutrophil membrane for targeted therapy of lymphoma

Biomimetic nanotherapy: core–shell structured nanocomplexes based on the neutrophil membrane for targeted therapy of lymphoma

A Nano-Traditional Chinese Medicine Against Lymphoma That Regulates the Level of Reactive Oxygen Species

Development of a nano-drug delivery system based on mesoporous silica and its anti-lymphoma activity

Contact Info

Product

Resources

About