Smart designing of new hybrid materials based on brushite-alginate and monetite-alginate microspheres: Bio-inspired for sequential nucleation and growth

Amer, Walid; Abdelouahdi, Karima; Ramananarivo, Hugo Ronald; Fihri, Aziz; Achaby, Mounir El; Zahouily, Mohamed; Barakat, Abdellatif; Djessas, K.; Clark, James H.; Solhy, Abderrahim

doi:10.1016/j.msec.2013.11.012

Cited by 15 publications

(16 citation statements)

References 54 publications

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“…The mineral front is trailing slightly behind the gelling front. proposed by Amer et al 32 , although with reversed Ca/P ratio and a smaller bead size. The reversed Ca/P ratio was needed to form stable alginate gels 43 .…”

Section: Investigating Parameters For Cap Precipitation Within Alginamentioning

confidence: 84%

“…30,31 A recent attempt was made by Amer et al to precipitate brushite in situ with alginate. 32 While mineral was formed within the hydrogel, little attention was paid to reaction conditions or biocompatibility which resulted in poorly defined composites and unstable gels. During preliminary experiments, we found that taking such an approach to the synthesis of a brushite alginate composite was unsatisfactory in terms of reaction control and reproducibility.…”

Section: Introductionmentioning

confidence: 99%

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Controlled mineralisation and recrystallisation of brushite within alginate hydrogels

Bjørnøy¹,

Bassett²,

Ucar³

et al. 2016

Biomed. Mater.

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Abstract. Due to high solubility and resorption behaviour under physiological conditions, brushite (CaHPO 4 ·2H 2 O, calcium monohydrogen phosphate dihydrate, dicalcium phosphate dihydrate) has great potential in bone regeneration applications, both in combination with scaffolds or as component of calcium phosphate cements. The use of brushite in combination with hydrogels opens possibilities for new cell based tissue engineering applications of this promising material. However, published preparation methods of brushite composites, in which the mineral phase is precipitated within the hydrogel network, fail to offer the necessary degree of control over mineral phase, content and distribution within the hydrogel matrix. The main focus of this study was to address these shortcomings by determining precise fabrication parameters needed to prepare composites with controlled composition and properties. Composite alginate microbeads were prepared using a counter-diffusion technique which allows for simultaneous crosslinking of the hydrogel and precipitation of an inorganic mineral phase. Reliable nucleation of a desired mineral phase within the alginate network proved more challenging than simple aqueous precipitation. This was largely due to ion transport within the hydrogel producing concentration gradients that modified levels of supersaturation and favoured the nucleation of other phases such as hydroxyapatite and octacalcium phosphate which would otherwise not form. To overcome this, incorporation of brushite seed crystals resulted in good control over the mineral phase and by adjusting the amount of seeds and precursor concentration, the amount of mineral could be tuned. The material has been characterized with a range of physical techniques, including scanning electron microscopy, powder X-ray diffraction and Rietveld refinement, Fourier transform infrared spectroscopy and thermogravimetric analysis, in order to assess mineral morphology, phase and amount within the organic matrix. The mineral content of the composite material converted from brushite into hydroxyapatite when submerged in simulated body fluid, indicating possible bioactivity. Additionally, initial cell culture studies revealed that both the material and the synthesis procedure is compatible with cells relevant to bone tissue engineering.

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Section: Investigating Parameters For Cap Precipitation Within Alginamentioning

confidence: 84%

Section: Introductionmentioning

confidence: 99%

Controlled mineralisation and recrystallisation of brushite within alginate hydrogels

Bjørnøy¹,

Bassett²,

Ucar³

et al. 2016

Biomed. Mater.

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show abstract

“…The stability and homogeneity of this gel can be explained by possible interactions between the HPO 4 2− moiety and the carboxylate group of alginate. This physical bonding translates to mixtures that are rich in electronic pairs leading to a higher reactivity and mineralization potential that can be transformed into composite materials [13]. According to X-ray diffraction data, synthesis in the presence of glutamic acids in the reaction medium maintained at pH 4.5 ± 0.5 yields the formation of dicalcium phosphate dihydrate (DCPD) in the printed samples (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…The H 2 O bands were observed to shift from 650 to 636 cm −1 . Apparently, calcium phosphate reacts with alginate through the carboxyl groups of amino acids and calcium in calcium phosphate, because the frequency changes are observed for groups located near calcium ions in the phosphate structures [13]. …”

Section: Resultsmentioning

confidence: 99%

3D printing of mineral–polymer bone substitutes based on sodium alginate and calcium phosphate

Egorov¹,

Fedotov²,

Миронов³

et al. 2016

Beilstein J. Nanotechnol.

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We demonstrate a relatively simple route for three-dimensional (3D) printing of complex-shaped biocompatible structures based on sodium alginate and calcium phosphate (CP) for bone tissue engineering. The fabrication of 3D composite structures was performed through the synthesis of inorganic particles within a biopolymer macromolecular network during 3D printing process. The formation of a new CP phase was studied through X-ray diffraction, Fourier transform infrared spectroscopy and scanning electron microscopy. Both the phase composition and the diameter of the CP particles depend on the concentration of a liquid component (i.e., the “ink”). The 3D printed structures were fabricated and found to have large interconnected porous systems (mean diameter ≈800 μm) and were found to possess compressive strengths from 0.45 to 1.0 MPa. This new approach can be effectively applied for fabrication of biocompatible scaffolds for bone tissue engineering constructions.

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“…Therefore, it provides better access to calcium ions than HA [6]. DCPD is metastable under physiological conditions, is resorbed more easily than HA, and can be used as a precursor for bone mineralization [7,8].…”

Section: Introductionmentioning

confidence: 99%

Nano-porous calcium phosphate balls

Kovach

Kosmella

Prietzel

et al. 2015

Colloids and Surfaces B: Biointerfaces

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Smart designing of new hybrid materials based on brushite-alginate and monetite-alginate microspheres: Bio-inspired for sequential nucleation and growth

Cited by 15 publications

References 54 publications

Controlled mineralisation and recrystallisation of brushite within alginate hydrogels

Controlled mineralisation and recrystallisation of brushite within alginate hydrogels

3D printing of mineral–polymer bone substitutes based on sodium alginate and calcium phosphate

Nano-porous calcium phosphate balls

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