2021
DOI: 10.1017/s1431927621002129
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Smart EPU: SPA Getting Intelligent

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Cited by 7 publications
(7 citation statements)
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“…The image processing and ML routines from Ptolemy that were integrated together with the algorithms added into Leginon may also be integrated into other collection software packages, such as SerialEM 10 , UCSFImage4 11 , TFS EPU 12, 13 , Gatan Latitude, JEOL JADAS 14 , and AutoEMation 15 . We describe in the Materials and Methods the exact modifications required to integrate Ptolemy into Leginon and to perform filtering and grid manipulation.…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…The image processing and ML routines from Ptolemy that were integrated together with the algorithms added into Leginon may also be integrated into other collection software packages, such as SerialEM 10 , UCSFImage4 11 , TFS EPU 12, 13 , Gatan Latitude, JEOL JADAS 14 , and AutoEMation 15 . We describe in the Materials and Methods the exact modifications required to integrate Ptolemy into Leginon and to perform filtering and grid manipulation.…”
Section: Discussionmentioning
confidence: 99%
“…Current data collection software packages available to the public (e.g. Leginon 8, 9 , SerialEM 10 , UCSFImage4 11 , TFS EPU 12, 13 , Gatan Latitude, JEOL JADAS 14 , AutoEMation 15 ) focus on exhausting the usable imaging area on a single grid. This is commonly achieved through a high degree of tuning of automated targeting parameters.…”
Section: Introductionmentioning
confidence: 99%
“…The image processing and ML routines from Ptolemy that were integrated together with the algorithms added into Leginon may also be integrated into other collection software packages, such as SerialEM (Mastronarde, 2003), UCSF-Image4 (Li et al, 2015), TFS EPU (Deng et al, 2021;Drulyte et al, 2022), Gatan Latitude, Jeol JADAS (Zhang et al, 2009) and AutoEMation (Lei & Frank, 2005). We describe in the Mate-rials and Methods the exact modifications required to integrate Ptolemy into Leginon and to perform filtering and grid manipulation.…”
Section: Potential For Implementation In Other Collection Softwarementioning
confidence: 99%
“…Current data-collection software packages available to the public [e.g. Leginon (Cheng et al, 2021;Suloway et al, 2005), SerialEM (Mastronarde, 2003), UCSFImage4 (Li et al, 2015), TFS EPU (Drulyte et al, 2022;Deng et al, 2021), Gatan Latitude and JEOL JADAS (Zhang et al, 2009), and Auto-EMation (Lei & Frank, 2005)] focus on exhausting the usable imaging area on a single grid. This is commonly achieved through a high degree of tuning of automated targeting parameters.…”
Section: Introductionmentioning
confidence: 99%
“…Previously, Smart Leginon Autoscreen and the underlying machine learning software, Ptolemy, have been introduced, and their underlying methods and algorithms together with examples have been described 8,9 . Several other software packages are either capable of or working towards fully automated multi-grid screening 10 , including SmartScope 11 , Smart EPU 12 , and CryoRL 13,14 . To address the screening bottleneck, Smart Leginon allows the user to first set up screening parameters in a template microscope session, then use that template session's parameters as a template to screen the full cassette of grids in the microscope autoloader.…”
Section: Introductionmentioning
confidence: 99%