Smart surface imprinting polymer nanospheres for selective recognition and separation of glycoprotein

Gao, Fengxian; Ma, Xiaotong; He, Xiwen; Li, Wen‐You; Zhang, Yukui

doi:10.1016/j.colsurfa.2013.05.018

Cited by 56 publications

(23 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The combination of the boronic acid group with thermosensitive imprinted layers led to a dual-responsive system capable of recognizing the target glycoprotein in response to changes in pH and temperature, which has great potential applications in chemical sensing and bio-separation. 300 Besides, Tang et al 301 described a molecularly imprinted fluorescence nanosensor for monitoring glycoproteins based on boronate affinity and thermo-sensitivity. The properties and performance of the prepared nanosensor for glycoproteins were regulated by controlling the pH value and temperature.…”

Section: View Article Onlinementioning

confidence: 99%

Molecular imprinting: perspectives and applications

et al. 2016

View full text Add to dashboard Cite

a Molecular imprinting technology (MIT), often described as a method of making a molecular lock to match a molecular key, is a technique for the creation of molecularly imprinted polymers (MIPs) with tailor-made binding sites complementary to the template molecules in shape, size and functional groups. Owing to their unique features of structure predictability, recognition specificity and application universality, MIPs have found a wide range of applications in various fields. Herein, we propose to comprehensively review the recent advances in molecular imprinting including versatile perspectives and applications, concerning novel preparation technologies and strategies of MIT, and highlight the applications of MIPs. The fundamentals of MIPs involving essential elements, preparation procedures and characterization methods are briefly outlined.Smart MIT for MIPs is especially highlighted including ingenious MIT (surface imprinting, nanoimprinting, etc.), special strategies of MIT (dummy imprinting, segment imprinting, etc.) and stimuli-responsive MIT (single/dual/ multi-responsive technology). By virtue of smart MIT, new formatted MIPs gain popularity for versatile applications, including sample pretreatment/chromatographic separation (solid phase extraction, monolithic column chromatography, etc.) and chemical/biological sensing (electrochemical sensing, fluorescence sensing, etc.). Finally, we propose the remaining challenges and future perspectives to accelerate the development of MIT, and to utilize it for further developing versatile MIPs with a wide range of applications (650 references).

show abstract

Section: View Article Onlinementioning

confidence: 99%

Molecular imprinting: perspectives and applications

et al. 2016

View full text Add to dashboard Cite

show abstract

“…10.0 g of commercially available milk samples was dispersed into 1.0 mL of trichloroacetic acid (15% in water), and the blended solution was stirred at 298 K for 1.0 h. Then the sample was centrifugated for 15 min at 2000 rpm rotating speed, and filtrated through 0.45 mm filter membrane to remove the denatured proteins. After centrifugation and filtration, the extraction solution was collected and spiked with 2,4,6-TCP at levels of 50 mg L À1 .…”

Section: Sample Preparation and Spe Proceduresmentioning

confidence: 99%

“…Lv et al prepared SMIPs for selective recognition biomacromolecules [9]. Gao et al prepared SMIPs for selective separation glycoprotein [10]. Generally, the substrate materials used in the SMIPs are magnetic nanoparticles [11], carbon nanotubes [12] and silica beads [13].…”

Section: Introductionmentioning

confidence: 99%

Magnetic imprinted nanomicrosphere attached to the surface of bacillus using miniemulsion polymerization for selective recognition of 2,4,6-trichlorophenol from aqueous solutions

Sun

et al. 2015

Journal of Industrial and Engineering Chemistry

View full text Add to dashboard Cite

“…Thus, protein molecularly imprinted polymers with high specificity adsorption and selectivity for the target proteins (Lian and Wang, ) can be used in proteomics and to achieve separation and enrichment of low‐abundance proteins from high‐abundance proteins. The most widely used techniques for preparing MIPs include entrapment (Huang et al ., ), freeze‐drying (Slade, ), surface imprinting (Gao et al ., ), the epitope approach (Rachkov and Minoura, ) and affinity blotting (Vaidya et al ., ).…”

Section: Introductionmentioning

confidence: 99%

Preparation of lysozyme molecularly imprinted polymers and purification of lysozyme from egg white

Wang

Dong

Bai

2014

Biomedical Chromatography

View full text Add to dashboard Cite

Molecular imprinting as a promising and facile separation technique has received much attention because of its high selectivity for target molecules. In this study, lysozyme molecularly imprinted polymers (Lys-MIPs) were successfully prepared by the entrapment method with lysozyme as the template molecule, acrylamide as the functional monomer and N,N-methylenebisacrylamide as the cross-linker. The removal of the template lysozyme from the molecularly imprinted polymers was investigated in detail by two methods. The synthesized Lys-MIPs were characterized by scanning electron microscopy and Fourier transform-infrared, and the adsorption capacity, selectivity and reproducibility of the Lys-MIPs were also evaluated. The maximum adsorption capacity reached 94.8 mg/g, which is twice that of nonmolecularly imprinted polymers, and satisfactory selectivity and reproducibility were achieved. Using the Lys-MIP column, lysozyme could be separated completely from egg white, with purity close to 100% and mass recovery of 98.2%. This illustrated that the synthesized Lys-MIPs had high specific recognition and selectivity to the template lysozyme when they were applied to a mixture of protein standards and a real sample.

show abstract

Smart surface imprinting polymer nanospheres for selective recognition and separation of glycoprotein

Cited by 56 publications

References 45 publications

Molecular imprinting: perspectives and applications

Molecular imprinting: perspectives and applications

Magnetic imprinted nanomicrosphere attached to the surface of bacillus using miniemulsion polymerization for selective recognition of 2,4,6-trichlorophenol from aqueous solutions

Preparation of lysozyme molecularly imprinted polymers and purification of lysozyme from egg white

Contact Info

Product

Resources

About