Smear layer removal by different chemical solutions used with or without ultrasonic activation after post preparation

Poletto, Daniel; Poletto, Ana Claudia; Cavalaro, Andressa; Machado, Ricardo; Cosme‐Silva, Leopoldo; Garbelini, Cássia Cilene Dezan; Hoeppner, Márcio Grama

doi:10.5395/rde.2017.42.4.324

Cited by 14 publications

(19 citation statements)

References 31 publications

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“…Estrela et al [41] described the tissue dissolving mechanism of NaOCl associated with (a) saponification reaction to degrade fatty acids and (b) chloramination reaction to release chlorine, which form chloramines in reaction with proteins. Even with strong tissue dissolving capability, the NaOCl cannot remove the smear layer effectively [42]. This tissue-dissolving property was confirmed by our observation of the nearly full dissolution of zebrafish embryo in 1.5% NaOCl within 5 min.…”

Section: Discussionsupporting

confidence: 70%

The Antibacterial Efficacy and In Vivo Toxicity of Sodium Hypochlorite and Electrolyzed Oxidizing (EO) Water-Based Endodontic Irrigating Solutions

et al. 2020

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The objective of this study was to evaluate the antibacterial efficacy against Enterococcus faecalis and Streptococcus mutans and in vivo toxicity using embryonic zebrafish assays of sodium hypochlorite (NaOCl) and electrolyzed oxidizing (EO) water (containing hypochlorous acid (HOCl))-based root canal irrigating solutions. Methodology: Using 100 µL microbial count of 1 × 10 8 cfu/mL Enterococcus faecalis to mix with each 10 mL specimen of NaOCl or HOCl for designed time periods. The above protocol was also repeated for Streptococcus mutans. The concentration of viable microorganisms was estimated based on each standardized inoculum using a plate-count method. Zebrafish embryo assays were used to evaluate acute toxicity. Results: All the HOCl or NaOCl treatment groups showed > 99.9% antibacterial efficacy against Enterococcus faecalis and Streptococcus mutans. Zebrafish embryos showed almost complete dissolution in 1.5% NaOCl within 5 min. Both survival rates after being treated with 0.0125% and 0.0250% HOCl for 0.5 min or 1.0 min were similar to that of E3 medium. Conclusions: Both NaOCl and HOCl revealed similar antibacterial efficacy (> 99.9%) against Enterococcus faecalis and Streptococcus mutans. While 1.5% NaOCl fully dissolved the Zebrafish embryos, both 0.0125% and 0.0250% HOCl showed little in vivo toxicity, affirming its potential as an alternative irrigation solution for vital pulp therapy.

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Section: Discussionsupporting

confidence: 70%

The Antibacterial Efficacy and In Vivo Toxicity of Sodium Hypochlorite and Electrolyzed Oxidizing (EO) Water-Based Endodontic Irrigating Solutions

et al. 2020

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“…Earlier investigations used light microscopy to identify the smear layer on the canal walls, but today SEM analysis is the standard in the field of quantitative and qualitative estimation of the presence of the smear layer due to high resolution and high magnification [22][23][24][25].…”

Section: Discussionmentioning

confidence: 99%

“…Such good results can be explained by the fact that instrumented canals were straight and simple, and adequate diameters of apical preparation (30/04) ensures that the tip of the irrigation needle will reach almost the working length of the instrumentation and in this way effectively clean the walls of the root canal. It also explains very good results for chlorhexidine, which, unlike NaOCl and citric acid, does not have the ability to dissolve tissues, but it is used because of a wide antibacterial spectrum (including Enterococcus faecalis) and prolonged antimicrobial effect [8,12,13,23].…”

Section: Discussionmentioning

confidence: 99%

The influence of the final irrigation protocol on the efficiency of root canal cleaning

et al. 2020

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Introduction/Objective Irrigation has an important role in root canal cleaning and its efficiency depends on the type of irrigants, the amount, the technique and the irrigation protocol. The aim of this work was to estimate the efficiency of cleaning of the canal walls by using scanning electron microscope analysis after the instrumentation by rotary NiTi instruments with the use of three different irrigation solutions and two final irrigation protocols. Methods Sixty extracted human incisors were divided into two groups after the rotary instrumentation with the iRace instruments. In both groups, the same amount (1.5 ml) of three solutions (2% sodium hypochlorite solution, 2% chlorhexidine solution, and 10% citric acid solution) and total final irrigation time (90 seconds) was the same. The final irrigation in the first group was accomplished using the technique of continuous irrigation and in the second group it was done using the intermittent protocol. The roots were cut longitudinally and analyzed by thirds (coronal, middle, and apical) on a scanning electron microscope (JSM 6460LV, JEOL Ltd., Tokyo, Japan) with 1,000× magnification. Results The most efficient cleaning of the root canal walls in both groups was seen after the use of citric acid with the intermittent protocol of the final irrigation (90.7% clean walls), while the least efficient was the final irrigation by chlorhexidine with continuous irrigation (80.3%). The most efficient cleaning of the canal walls in both groups was observed in the coronal third and the largest amount of the smear layer in the apical third. Conclusion The most efficient cleaning of the canal was achieved by the use of citric acid and the intermittent protocol of the final irrigation. In all tested solutions, the intermittent protocol of irrigation was more efficient than continuous irrigation.

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“…2,18 So, other acidic agents such as EDTA and polyacrylic acid were developed to be used. 9,18,19 These conditioning agents stimulate partial elimination of the smear layer only without any change in the mineral phase of the dentin, which improves the chemical interaction between the cement layer and the substrate. Removal of the smear layer using polyacrylic acid performed safely without significantly etching the dentin.…”

Section: Introductionmentioning

confidence: 99%

Impact of Dentin Conditioning on Shear Bond Strength of Self-Adhesive Resin Cement

Ibraheim

2018

Egyptian Dental Journal

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Statement of the Problem:The clinical success of fixed dental prosthesis depends on the bond strength between the prepared tooth and the luting cement.Purpose: To estimate the bond strength of self-adhesive resin cement to dentin of prepared teeth using different conditioning methods. Materials and Methods:Mandibular first molars (n=32) periodontally compromised and indicated for extraction were collected after patient consent from oral surgery department, Faculty of Dentistry, Mansoura University. Flat dentin surfaces were prepared and treated as follows: (1) no pretreatment of dentin (T-NT) control group; (2) self-etching bonding agent (T-SEB); (3) Dentin conditioning agent (polyacrylic acid) (T-PA) (4) Ethylenediaminetetraacetic acid (EDTA) (T-ED). Self-adhesive resin cement (SuperCem), was used to lute discs of composite on conditioned prepared teeth surfaces. Specimens were stored in distilled water at 37˚C for 24 hours after that thermal cycling was performed between 5°C and 50°C in distilled water with a dwell time of 1 minute (Theromocycler, Robota, Alexandria, Egypt) for 10.000 cycles. Shear bond strength (SBS) was measured in MPa using a universal testing machine at a crosshead speed of 0.5 mm/min. Scanning electron microscope (SEM) was used for failure modes examination.

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Smear layer removal by different chemical solutions used with or without ultrasonic activation after post preparation

Cited by 14 publications

References 31 publications

The Antibacterial Efficacy and In Vivo Toxicity of Sodium Hypochlorite and Electrolyzed Oxidizing (EO) Water-Based Endodontic Irrigating Solutions

The Antibacterial Efficacy and In Vivo Toxicity of Sodium Hypochlorite and Electrolyzed Oxidizing (EO) Water-Based Endodontic Irrigating Solutions

The influence of the final irrigation protocol on the efficiency of root canal cleaning

Impact of Dentin Conditioning on Shear Bond Strength of Self-Adhesive Resin Cement

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