The study analyzes the development of business incubation literature in South Africa. The VOSviewer software was used to perform the bibliometric analysis of 44 articles on business incubation in South Africa conducted between 1996 and 2023 that were obtained from the Scopus database. The study has also revealed literature on business incubation started to appear in 1996 in South Africa. The study’s keyword co-occurrence analysis also revealed that the following words had the most occurrences and were clustered into the following: entrepreneurship and business incubation, business incubators and effectiveness, entrepreneurial education and informal Business, SMEs and start-up, business and sustainability, and business development and competitiveness. The study sorts the articles based on the following indicators: number of documents published in a year, eminent authors and keyword co-occurrence, which identified six categories of the most prominent research themes being covered by articles reviewed. The study only used articles that were written in English language only. Therefore, future research should consider articles written in other languages and expand the study to other regions, continents, and even global scale.