Smilax china L. Polysaccharide Alleviates Dextran Sulphate Sodium-Induced Colitis and Modulates the Gut Microbiota in Mice

Abstract

This work aimed to investigate the preventive effect of Smilax china L. polysaccharide (SCP) on dextran sulfate sodium (DSS)-induced ulcerative colitis (UC) in mice. Smilax china L. polysaccharide was isolated by hot water extraction, ethanol precipitation, deproteinization, and purification using DEAE-cellulose column chromatography to yield three polysaccharides: SCP_C, SCP_A, and SCP_N. Acute colitis was induced by administering 3% (w/v) DSS in drinking water for 7 days. Sulfasalazine, SCP_C, SCP_A, and SCP_N were administered by gavage for 9 days. SCP_C, SCP_A, and SCP_N could significantly improve symptoms, as evidenced by the declining disease activity index (DAI), decreased spleen weight, increased length of the colon, and improved colonic histology. Moreover, SCP_C, SCP_A, and SCP_N increased serum glutathione and decreased the levels of pro-inflammatory cytokines, malondialdehyde, nitric oxide, and myeloperoxidase in colon tissues. Additionally, SCP_C, SCP_A, and SCP_N modulated gut microbiota via ascending the growth of Lachnospiraceae, Muribaculaceae, Blautia, and Mucispirillum and descending the abundance of Akkermansiaceae, Deferribacteraceae, and Oscillibacter in mice with UC. The results suggested that Smilax china L. polysaccharide ameliorates oxidative stress, balances inflammatory cytokines, and modulates gut microbiota, providing an effective therapeutic strategy for UC in mice.

Citrus tangerine pith extract alleviates hypoxia-induced ileum damage in mice by modulating intestinal microbiota

“…27 Remarkably, Firmicutes play a key role in gut homeostasis by producing metabolites, and their abundance promotes anti-inflammatory and anti-tumorigenic properties. 47 At the genus level, compared with the BH group, CTPE treatment significantly increased the abundance of Lactobacillus . Lactobacillus is a common probiotic, which can ferment carbohydrates to produce lactic acid, promote the digestion and absorption of nutrients, acidify the intestinal environment, and prevent the adhesion of harmful bacteria in the intestinal epithelium.…”

The Effects of Synbiotics on Dextran-Sodium-Sulfate-Induced Acute Colitis: The Impact of Chitosan Oligosaccharides on Endogenous/Exogenous Lactiplantibacillus plantarum

“…The abundance of Muribaculaceae is strongly correlated with propionic acid concentration [43] and negatively correlated with proinflammatory cytokines TNF-α, IL-6, and IL-1β [44]. The Lachnospiraceae_NK4A136_group reduces intestinal inflammation and diarrhea [45] and facilitates the recovery of colitis [46]. Lactobacillus is involved in regulating intestinal inflammation and the epithelial barrier of the host by affecting tryptophan metabolism [47].…”