Smilax weniae, a New Species of Smilacaceae from Limestone Areas Bordering Guizhou and Guangxi, China

Feng, Jie-Ying; Jin, Xinjie; Zhang, Sheng-Lu; Yang, Jia-Wen; Fei, Shi-Peng; Huang, Yu‐Song; Liu, Yan; Qi, Zhe-Chen; Li, Pan

doi:10.3390/plants11081032

Cited by 9 publications

(8 citation statements)

References 22 publications

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“…Following the manufacturer’s directions, DNA Plantzol Reagent (Invitrogen, Carlsbad, CA, USA) was used to extract the total DNA from the plant’s leaves [ 75 , 76 , 77 ]. Utilizing Illumina’s TruSeq Nano DNA Library Preparation kit (350 bp median insert), sequencing libraries were created in accordance with the manufacturer’s instructions [ 78 , 79 , 80 ]. The Illumina HiSeq 2500 platform was used to produce the chloroplast sequences (Illumina Inc., San Diego, CA, USA).…”

Section: Methodsmentioning

confidence: 99%

Characterization and Comparative Analysis of Chloroplast Genomes in Five Uncaria Species Endemic to China

Chen

Zhang

Liang

et al. 2022

IJMS

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Uncaria, a perennial vine from the Rubiaceae family, is a typical Chinese traditional medicine. Currently, uncertainty exists over the Uncaria genus’ evolutionary relationships and germplasm identification. The complete chloroplast genomes of four Uncaria species mentioned in the Chinese Pharmacopoeia and Uncaria scandens (an easily confused counterfeit) were sequenced and annotated. The findings demonstrated that the whole chloroplast genome of Uncaria genus is 153,780–155,138 bp in full length, encoding a total of 128–131 genes, containing 83–86 protein-coding genes, eight rRNAs and 37 tRNAs. These regions, which include eleven highly variable loci and 31–49 SSRs, can be used to create significant molecular markers for the Uncaria genus. The phylogenetic tree was constructed according to protein-coding genes and the whole chloroplast genome sequences of five Uncaria species using four methods. The topology of the two phylogenetic trees showed no difference. The sequences of U. rhynchophylla and U. scandens are clustered in one group, while the U. hirsuta and U. macrophylla are clustered in another group. U. sessilifructus is clustered together with the above two small clades. New insights on the relationship were revealed via phylogenetic research in five Uncaria species. This study will provide a theoretical basis for identifying U. rhynchophylla and its counterfeits, as well as the species of the Uncaria genus. This research provides the initial chloroplast genome report of Uncaria, contributes to elucidating the chloroplast genome evolution of Uncaria in China.

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Section: Methodsmentioning

confidence: 99%

Characterization and Comparative Analysis of Chloroplast Genomes in Five Uncaria Species Endemic to China

Chen

Zhang

Liang

et al. 2022

IJMS

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“…Diploid controls and tissue culture plantlets that were determined to be tetraploid by flow cytometry were transferred to rooting medium (MS + 0.5 mg/L NAA) for further cultivation. The ploidy of tissue culture plantlets was verified by root tip squash method [ 45 ]. When the roots were 1–2 cm in length, the root tips were collected at 10:00 a.m., pretreated with 0.1% colchicine solution for 3 h at 4 °C, washed with distilled water, and then fixed in FAA solution (formalin: glacial acetic acid: 70% ethanol = 5: 5: 90, v: v: v) for 24 h at 4 °C.…”

Section: Methodsmentioning

confidence: 99%

In vitro induction of tetraploids and their phenotypic and transcriptome analysis in Glehnia littoralis

Zhang,

Zheng,

Wang

et al. 2024

BMC Plant Biol

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Background Glehnia littoralis is a medicinal and edible plant species having commercial value and has several hundred years of cultivation history. Polyploid breeding is one of the most important and fastest ways to generate novel varieties. To obtain tetraploids of G. littoralis in vitro, colchicine treatment was given to the seeds and then were screened based on morphology, flow cytometry, and root tip pressing assays. Furthermore, transcriptome analysis was performed to identity the differentially expressed genes associated with phenotypic changes in tetraploid G. littoralis. Results The results showed that 0.05% (w/v) colchicine treatment for 48 h was effective in inducing tetraploids in G. littoralis. The tetraploid G. littoralis (2n = 4x = 44) was superior in leaf area, leaf thickness, petiole diameter, SPAD value (Chl SPAD), stomatal size, epidermal tissues thickness, palisade tissues thickness, and spongy tissues thickness to the diploid ones, while the stomatal density of tetraploids was significantly lower. Transcriptome sequencing revealed, a total of 1336 differentially expressed genes (DEGs) between tetraploids and diploids. Chromosome doubling may lead to DNA content change and gene dosage effect, which directly affects changes in quantitative traits, with changes such as increased chlorophyll content, larger stomata and thicker tissue of leaves. Several up-regulated DEGs were found related to growth and development in tetraploid G. littoralis such as CKI, PPDK, hisD and MDP1. KEGG pathway enrichment analyses showed that most of DEGs were enriched in metabolic pathways. Conclusions This is the first report of the successful induction of tetraploids in G. littoralis. The information presented in this study facilitate breeding programs and molecular breeding of G. littoralis varieties.

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“…Plastome Dataset —Low quality reads and adapters were trimmed with fastp (Chen et al 2018). Reference-guided assemblies were carried out in Geneious (Biomatters; Auckland, New Zealand) with the complete chloroplast genome of Smilax weniae P.Li, Z.C.Qi & Yan Liu from Feng et al (2022), downloaded from GenBank (NC_067604), as the reference. Reference assemblies were then used to generate majority consensus sequences, which we added to a larger dataset of plastome sequences of Smilax downloaded from GenBank (Appendix 1).…”

Section: Methodsmentioning

confidence: 99%

A New Endemic Smilax (Smilacaceae) from Tafelberg, Suriname, Supported by Morphometric and Phylogenetic Evidence

Murphy,

Heaton,

Molgo

et al. 2023

Systematic Botany

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Abstract— Taxonomy of Smilax in the Neotropics is poorly known with many undescribed species, unresolved synonymy, and regional treatments with conflicting taxon concepts. This issue is amplified in the Guiana Shield, which has arguably received the least amount of taxonomic research in Smilax. Here, we investigate multiple specimens from Tafelberg, Suriname that we hypothesized to represent an undescribed species. We used univariate and multivariate morphometrics to analyze linear measurements and leaf outlines to test if and how Tafelberg specimens differ from those of morphologically similar species in the Guiana Shield, Smilax lasseriana and S. maypurensis. We used a genome skimming approach to sequence and assemble plastomes, which also allowed us to extract plastid loci previously employed in Sanger-based studies and place the putative new species in a phylogeny with expanded taxon sampling. We recovered the Smilax from Tafelberg as more closely related to species with ranges centered in eastern Brazil than to its morphologically hypothesized congeners, S. lasseriana and S. maypurensis. We describe this new species as Smilax tafelbergensis with detailed morphological comparisons to similar taxa, a distribution map, and illustration.

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Smilax weniae, a New Species of Smilacaceae from Limestone Areas Bordering Guizhou and Guangxi, China

Cited by 9 publications

References 22 publications

Characterization and Comparative Analysis of Chloroplast Genomes in Five Uncaria Species Endemic to China

Characterization and Comparative Analysis of Chloroplast Genomes in Five Uncaria Species Endemic to China

In vitro induction of tetraploids and their phenotypic and transcriptome analysis in Glehnia littoralis

A New Endemic Smilax (Smilacaceae) from Tafelberg, Suriname, Supported by Morphometric and Phylogenetic Evidence

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