2012
DOI: 10.1158/1535-7163.mct-12-0107
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Smoking Induces Epithelial-to-Mesenchymal Transition in Non–Small Cell Lung Cancer through HDAC-Mediated Downregulation of E-Cadherin

Abstract: Epidemiological studies have demonstrated that most cases of lung cancers (85-90%) are directly attributable to tobacco smoking. Although association between cigarette smoking and lung cancer is well documented, surprisingly little is known regarding the molecular mechanisms of how smoking is involved in epithelial-to-mesenchymal transition (EMT) through epigenetic changes. Here we show that lung cancer patients with a smoking history have low E-cadherin (E-cadh) levels and loss of E-cadh is a poor prognostic … Show more

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Cited by 80 publications
(64 citation statements)
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“…To address this possibility, the control GFP and GFP-TLE1 pool of cells were treated with or without the HDAC inhibitor, Trichostatin A (TSA), and examined for E-cadherin expression. Consistent with previous finding (20), treatment of control A549 cells with TSA resulted in induction of TLE1 expression (Figs. 3E and 3F).…”
Section: Resultssupporting
confidence: 93%
See 1 more Smart Citation
“…To address this possibility, the control GFP and GFP-TLE1 pool of cells were treated with or without the HDAC inhibitor, Trichostatin A (TSA), and examined for E-cadherin expression. Consistent with previous finding (20), treatment of control A549 cells with TSA resulted in induction of TLE1 expression (Figs. 3E and 3F).…”
Section: Resultssupporting
confidence: 93%
“…Subsequent downstream steps were conducted following the protocol from the EpiSeeker ChIP Kit (Abcam). The PCRs were conducted using the E-cadherin primers (5'-CCCACCACGTACAAGGGTC-3` (sense), 5'-ATGCCATCGTTGTTCACTGGA-3` (antisense)) with the following program: 45 cycles at 95°C for 30 s, 56°C for 30s, and 72°C for 30s as described previously (20). The amplified DNA fragment of the E-cadherin promoter was separated on 1.5% agarose gel and visualized with ethidium bromide.…”
Section: Methodsmentioning
confidence: 99%
“…Recent work identified extensive genomic changes in DNA methylation in small airway epithelium (SAE) of smokers compared to smokers with corresponding modulation of gene expression [264]. Other recent studies have shown how cigarette smoke influences histone modifications and chromatin accessibility [265][266][267].…”
Section: Introductionmentioning
confidence: 99%
“…Through EMT, tumor cells are seeded in a location, and finally form metastatic cancer tissues whose morphology is similar to that of the primary tumor (Thiery, 2002;Thiery et al, 2006). Recent researches indicated that the abnormal EMT is closely associated with epithelial tumors, such as breast cancer (Zhang et al, 2012), lung cancer (Nagathihalli et al, 2012), colon carcinoma (Bellovin et al, 2005), prostate cancer (Shiota et al, 2012), cervical cancer , and gastric cancer (Jia et al, 2013).…”
Section: Introductionmentioning
confidence: 99%