2021
DOI: 10.1096/fj.202001759r
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SMURF2‐mediated ubiquitin signaling plays an essential role in the regulation of PARP1 PARylating activity, molecular interactions, and functions in mammalian cells

Abstract: Poly(ADP-ribose) polymerase 1 (PARP1) is a key molecular stress sensor and response mediator implicated in multiple cellular functions in health and diseases. Despite its importance and intrinsic involvement in pivotal molecular and cellular processes, including DNA repair, transcription regulation, chromatin organization, and cell death, the regulatory mechanisms of PARP1 are poorly understood. In this study, we show that SMURF2, a HECT-type E3 ubiquitin ligase and suggested tumor suppressor, physically inter… Show more

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Cited by 7 publications
(10 citation statements)
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“…Ubiquitination of protein IDRs is well studied, and evolutionarily conservation of Ub sites within IDRs is favored over ordered regions ( 91 ), although the effects of ion channel IDR ubiquitination on channel function are not well explored ( 33 , 87 ). However, there are numerous examples of monoubiquitination regulating protein function through alteration of protein–protein and protein–lipid interactions in other contexts ( 18 , 19 , 20 , 92 , 93 ), suggesting that monoubiquitination of the TRPV4 N-terminal IDR may similarly influence channel activity through effects on protein or membrane lipid interactions without affecting surface localization.…”
Section: Discussionmentioning
confidence: 99%
“…Ubiquitination of protein IDRs is well studied, and evolutionarily conservation of Ub sites within IDRs is favored over ordered regions ( 91 ), although the effects of ion channel IDR ubiquitination on channel function are not well explored ( 33 , 87 ). However, there are numerous examples of monoubiquitination regulating protein function through alteration of protein–protein and protein–lipid interactions in other contexts ( 18 , 19 , 20 , 92 , 93 ), suggesting that monoubiquitination of the TRPV4 N-terminal IDR may similarly influence channel activity through effects on protein or membrane lipid interactions without affecting surface localization.…”
Section: Discussionmentioning
confidence: 99%
“…Breast carcinoma MDA-MB-468 cells were a gift from Prof. Izhak Haviv. All cell lines, except for the IMR90 cells, were cultured in high glucose DMEM (Biological Industries, Beit-Haemek, Israel) supplemented with 2 mM L-glutamine, 10% ( v / v ) fetal bovine serum, and 1% ( v / v ) penicillin–streptomycin [ 34 , 36 , 37 , 38 ]. IMR90 cells were grown in RPMI 1640 medium (Biological Industries) supplemented with 2 mM L-glutamine, 15% ( v / v ) fetal bovine serum, and 1% ( v / v ) penicillin–streptomycin.…”
Section: Methodsmentioning
confidence: 99%
“…Transient protein expression was carried out by using either polyethyleneimine (PEI, Sigma-Aldrich, St. Louis, MO, USA) or FuGENE ® 6 (E2692, Promega Corporation, Madison, WI, USA) according to the manufacturer’s protocol. GFP-SMURF2 stably expressing cells were generated as described [ 37 ].…”
Section: Methodsmentioning
confidence: 99%
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