Snail Mucus Filtrate Reduces Inflammation in Canine Progenitor Epidermal Keratinocytes (CPEK)

Abstract

Atopic dermatitis (AD) is an inflammatory and allergic disease, whose multifactorial etiopathogenesis is the consequence of the link between the genetic, immunological and environmental components. The complexity and difficulty in understanding the causes that trigger or exacerbate this pathology makes it difficult, once diagnosed, to proceed with a targeted and effective therapeutic process. Today, the new frontiers of research look to natural and innovative treatments to counteract the different manifestations of dermatitis. From this point of view, the mucus secreted by Helix aspersa Muller has proven, since ancient times, to be able to neutralize skin diseases. To study canine atopic dermatitis (cAD), we used cell lines of canine epidermal keratinocytes (CPEK) that are optimal to understand the biological reactivity of keratinocytes in vitro. The data obtained from our study demonstrate the anti-inflammatory capacity of snail secretion filtrate (SSF) in counteracting the production of proinflammatory cytokines produced during cAD, highlighting the opportunities for further studies to be able to identify new, natural and safe treatments for cAD and to open new frontiers for veterinarians and owners.

Analysis of the Influence of IL-6 and the Activation of the Jak/Stat3 Pathway in Fibromyalgia

“…Real-time PCR analysis was performed by the SYBR Green method using the QuantiTect Primer Assay (Qiagen) with b-actin as internal control [ 73 ]. Real-time PCR was performed using a Bio-Rad CFX Real-Time PCR ((Bio-Rad, Milano, Italy) Detection System [ 74 ].…”

Gastropod Mucus: Interdisciplinary Perspectives on Biological Activities, Applications, and Strategic Priorities

“…Extracts increased skin hydration and reduced trans-epidermal water loss in animal studies, likely attributable to their moisturizing physical properties. Some bioactive compounds in gastropod mucus also exhibit antioxidant activities, including certain peptides and polyphenols. , In canine skin cell models, secretion filtrate from snail mucus reduced the production of inflammatory mediators implicated in atopic dermatitis, including cytokines like IL-6, IL-8, and IL-17A . These anti-inflammatory effects suggest the potential for snail mucus components as natural therapeutic agents for inflammatory skin conditions in veterinary species.…”

Cryptomphalus aspersa Egg Extract Protects against Human Stem Cell Stress-Induced Premature Senescence

“…First, we opted to assess CAEE's potential beneficial effects on would healing since this is an effect reported for other parts of the snail [26,28,31]. To that end, we performed a wound-healing assay (or cell-migration assay).…”

“…Snails secrete mucus for vital purposes. The mucus from Cryptomphalus aspersa is a lubricant with moisturizing, antimicrobial [24,25], anti-inflammatory [26], and healing capabilities [27,28]. These characteristics are derived from mucus bioactive compounds, such as allantoin glycolic acid, collagen, vitamins, minerals, and several antioxidant molecules [18,29].…”