Org. Biomol. Chem.
 2023
DOI: 10.1039/d3ob01003a
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SNAP-tagging live cells via chelation-assisted copper-catalyzed azide–alkyne cycloaddition

Daniel J. Stone,
Miguel Macias-Contreras,
Shaun M. Crist
et al.

Abstract: The sequential biochemical (SNAP-tag) and chemical (chelation-assisted copper-catalyzed azide–alkyne cycloaddition) reactions are applied in membrane protein labeling on live cells. The second, chemical step is rapid (within 1 minute) without any ill-effect to the labeled cells.

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