Snapshot Holographic Optical Tomography

Sung, Yongjin

doi:10.1103/physrevapplied.11.014039

Cited by 24 publications

(13 citation statements)

References 58 publications

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“…The most notable one is the projection approximation, which assumes that the light propagates along the direction of incident beam throughout the entire sample. The use of projection approximation may be justified by the low effective NA of lenslets [28]. In deriving Eq.…”

Section: Resultsmentioning

confidence: 99%

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Snapshot Three-Dimensional Absorption Imaging of Microscopic Specimens

Sung

2021

Phys. Rev. Applied

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Snapshot projection optical tomography (SPOT) uses a micro-lens array (MLA) to simultaneously capture the projection images of a three-dimensional (3D) specimen corresponding to different viewing directions. Compared to other light-field imaging techniques using an MLA, SPOT is dual telecentric and can block high-angle stray rays without sacrificing the light collection efficiency.Using SPOT, we recently demonstrated snapshot 3D fluorescence imaging. Here we demonstrate snapshot 3D absorption imaging of microscopic specimens. For the illumination, we focus the incoherent light from a light-emitting diode onto a pinhole, which is placed at a conjugate plane to the sample plane. SPOT allows us to capture the ray bundles passing through the specimen along different directions. The images recorded by an array of lenslets can be related to the projections of 3D absorption coefficient along the viewing directions of lenslets. Using a tomographic reconstruction algorithm, we obtain the 3D map of absorption coefficient. We apply the developed system to different types of samples, which demonstrates the optical sectioning capability. The transverse and axial resolutions measured with gold nanoparticles are 1.3 µm and 2.3 µm, respectively.

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Section: Resultsmentioning

confidence: 99%

“…(2b). Previously we demonstrated angular multiplexing of illumination beams for 3D snapshot imaging of refractive index map [28]. The technique called Snapshot holographic optical tomography (SHOT) uses an MLA to generate a multitude of collimated beams, each of which propagates through the sample along a different angle.…”

Section: Resultsmentioning

confidence: 99%

Snapshot Three-Dimensional Absorption Imaging of Microscopic Specimens

Sung

2021

Phys. Rev. Applied

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“…[72][73][74] So, at least in theory, a combined polarized, hyperspectral TDM system could, with proper data processing, emulate all kind of existing optical transmission microscopes, even the classical polarization microscope, which works in white light. Such an approach would be tremendously challenging in terms of instrumentation (speed of acquisition [75][76][77] and data processing [78][79][80] ), but could pave the way towards a kind of all-in-one, universal system for optical transmission microscopy.…”

Section: Conclusion and Outlooksmentioning

confidence: 99%

Multimodal image reconstruction from tomographic diffraction microscopy data

Abbessi

Verrier

Taddese

et al. 2022

Journal of Microscopy

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Tomographic diffraction microscopy (TDM) is a tool of choice for highresolution, marker-less 3D imaging of biological samples. Based on a generalization of digital holographic microscopy with full control of the sample's illumination, TDM measures, from many illumination directions, the diffracted fields in both phase and amplitude. Photon budget associated to TDM imaging is low. Therefore, TDM is not limited by phototoxicity issues. The recorded information makes it possible to reconstruct 3D refractive index distribution (with both refraction and absorption contributions) of the object under scrutiny, without any staining. In this contribution, we show an alternate use of this information. A tutorial for multimodal image reconstruction is proposed. Both intensity contrasts and phase contrasts are proposed, from the image formation model to the final reconstruction with both 2D and 3D rendering, turning TDM into a kind of 'universal' digital microscope.

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“…Using the MLA for angular multiplexing of illumination beams instead of deconvolving the light field after the sample has also been demonstrated. This method called Snapshot holographic optical tomography uses defocused imaging and digital holography to separately record the incident beams and restore the sharpness, respectively [ 17 ].…”

Section: Related Workmentioning

confidence: 99%

Hyperspectral Three-Dimensional Fluorescence Imaging Using Snapshot Optical Tomography

Juntunen

Woller

Sung

2021

Sensors

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Hyperspectral three-dimensional (3D) imaging can provide both 3D structural and functional information of a specimen. The imaging throughput is typically very low due to the requirement of scanning mechanisms for different depths and wavelengths. Here we demonstrate hyperspectral 3D imaging using Snapshot projection optical tomography (SPOT) and Fourier-transform spectroscopy (FTS). SPOT allows us to instantaneously acquire the projection images corresponding to different viewing angles, while FTS allows us to perform hyperspectral imaging at high spectral resolution. Using fluorescent beads and sunflower pollens, we demonstrate the imaging performance of the developed system.

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Snapshot Holographic Optical Tomography

Cited by 24 publications

References 58 publications

Snapshot Three-Dimensional Absorption Imaging of Microscopic Specimens

Snapshot Three-Dimensional Absorption Imaging of Microscopic Specimens

Multimodal image reconstruction from tomographic diffraction microscopy data

Hyperspectral Three-Dimensional Fluorescence Imaging Using Snapshot Optical Tomography

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