Introduction: The importance of socioeconomic variables such as level of literacy, income and occupational status and their impact on the physical and psychological wellbeing of the people is clear for experts and policymakers. In much research, the root of increase in life expectancy and improvement in other indexes of health is considered to not only progress in medicine, but also improve in socioeconomic indexes. Thus, the present study aims to determine the relation between socioeconomic status and general health and the consequences of disease on the quality of work life of the employees of Tehran University of Medical Sciences (TUMS). Methodology: The present cross-sectional research is of descriptive-analytical type, and was conducted in faculties of TUMS in 2015, and the population under study included all the 1,238 non-academic employees of the TUMS. The required data was collected by the Quality of Work life (QWL) questionnaire. This questionnaire was based on Walton components and Socioeconomic Status (SES) questionnaire, and was designed in order to evaluate socioeconomic status and has 4 components. The data on general health was collected by Goldberg and Hillier 28-Item General Health Questionnaire (GHQ-28) (1979) that has 4 subscales. Then, the collected data was recorded by SPSS version 18 software and was then analyzed by common methods of descriptive-analytical statistics. Results: The results demonstrated that the frequency of socioeconomic status of the employees under study were 179 persons (53.3 percent) for low level, 109 persons (35.5 percent) for moderate level, and 19 persons (6.2 percent) for high level, and the frequency of the quality of work life of the employees under study were 10 persons (3.3 percent) for low level, 108 persons (35.6 percent) for moderate level, and 185 persons (61.1 percent) for high level. Conclusion: Considering the importance of quality of work life in socioeconomic status, it is proposed that the following measures be taken into account: appropriateness of salary to economic factors such as inflation; demand and supply in fair and adequate payment; paying more attention to the physical conditions of workplace, e.g. light, cooling and heating facilities to prepare a secure and healthy workplace; preparing some possibilities for the employees so that they can further develop their personal talents and have opportunities for making progress in their specialized field by encouraging them to be creative and innovative to lead them to promotion in the organization; and providing continuous security and growth opportunities for the employees, allowing them to take initiatives, and provide them with any information or skill that they need in workplace to develop their human capabilities. In the present study no significant relationship between the quality of work life and general health, socioeconomic status and quality of work life, and also general health and socioeconomic status, was found.