SNAREs: Membrane Fusion and Beyond

Galli, Thierry; Tareste, David

doi:10.1016/b978-0-12-394447-4.20045-x

Cited by 2 publications

(2 citation statements)

References 93 publications

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“…We used TSPAN and v-SNARE proteins as fusion event reporters. Specifically, we relied on TSPAN CD9, CD63 and CD81 [32][33][34][35], and v-SNARE VAMP2/synaptobrevin2 (vesicleassociated membrane protein 2) and VAMP7/TI-VAMP (vesicle-associated membrane protein 7 / Tetanus neurotoxin-Insensitive vesicle-associated membrane protein) [36][37][38][39][40] proteins coupled to a pH-sensitive variant of GFP named pHluorin [10]. CD9-pHluorin, CD63-pHluorin and CD81-pHluorin plasmids were constructed as previously described by Verweij and colleagues [41].…”

Section: Reporters Of Fusion Events In Live Cellsmentioning

confidence: 99%

ExoJ: an ImageJ2/Fiji plugin for automated spatiotemporal detection and analysis of exocytosis

Liu

Verweij

Niel

et al. 2022

Preprint

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Exocytosis is a dynamic physiological process that enables the release of biomolecules to the surrounding environment via the fusion of membrane compartments to the plasma membrane. Defects in exocytosis could compromise essential biological functions so that it is crucial to elucidate the fundamentals of the exocytosis mechanisms. The development of pH-sensitive optical reporters alongside Total Internal Reflection Fluorescence (TIRF) microscopy has greatly expanded the knowledge of exocytosis, allowing the assessment of individual vesicle exocytosis events on a single-cell basis. The amount of data generated by live-cell imaging approaches poses the need of an analytical tool to identify and quantify the dynamics of exocytic events with high accuracy from fluorescent time series. Manual annotation represents a time-consuming task, and prone to selection biases and human operational errors. In this work, we report the development of ExoJ, an ImageJ2/Fiji-based plugin to automate the detection of exocytic events in single-cell studies. The pipeline of ExoJ entirely relies on a set of user-customizable parameters to identify and further quantify features of exocytic events such as the intensity, the apparent size and the duration of the process. ExoJ proposes a user-friendly interface with multiple optional tools to visualize features for individual exocytic events and/or the whole cell population events. To illustrate the capacity of the plugin, we recorded exocytic events reported by several tetraspanins (TSPANs) and vesicular soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptors (v-SNAREs) proteins, coupled to pHluorin. Using ExoJ, we characterize the dynamics of the exocytic event population reported by these two families of proteins. We showcase a similar fusion intensity but significantly distinct dynamics among TSPAN subpopulation. In particular, TSPAN CD9-pHluorin subpopulation displays a fast and similar dynamics to v-SNARE populations. ExoJ is a user-friendly plugin to detect exocytic events in a supervised manner. We designed ExoJ to be fully user-controllable at each step of the pipeline. We argue the user-oriented plugin is a useful tool to search, monitor and quantify the dynamic aspect of content release during exocytosis.

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Section: Reporters Of Fusion Events In Live Cellsmentioning

confidence: 99%

ExoJ: an ImageJ2/Fiji plugin for automated spatiotemporal detection and analysis of exocytosis

Liu

Verweij

Niel

et al. 2022

Preprint

View full text Add to dashboard Cite

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“…During the last two decades, significant progress has been made toward identifying the key molecular players and underlying biophysical mechanisms of membrane fusion machineries. The soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) proteins of neurotransmission and the hemagglutinin (HA) protein of the influenza virus have notably been the subject of many structural and functional studies, allowing a precise characterization of their mode of action 45– 48 . Both machineries use the energy released during the formation of a coiled-coil complex of α-helices to drive membrane fusion.…”

Section: Overview Of Cellular Membrane Fusion Machineriesmentioning

confidence: 99%

Recent insights into the structure and function of Mitofusins in mitochondrial fusion

Cohen

Tareste

2018

F1000Res

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Mitochondria undergo frequent fusion and fission events to adapt their morphology to cellular needs. Homotypic docking and fusion of outer mitochondrial membranes are controlled by Mitofusins, a set of large membrane-anchored GTPase proteins belonging to the dynamin superfamily. Mitofusins include, in addition to their GTPase and transmembrane domains, two heptad repeat domains, HR1 and HR2. All four regions are crucial for Mitofusin function, but their precise contribution to mitochondrial docking and fusion events has remained elusive until very recently. In this commentary, we first give an overview of the established strategies employed by various protein machineries distinct from Mitofusins to mediate membrane fusion. We then present recent structure–function data on Mitofusins that provide important novel insights into their mode of action in mitochondrial fusion.

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SNAREs: Membrane Fusion and Beyond

Cited by 2 publications

References 93 publications

ExoJ: an ImageJ2/Fiji plugin for automated spatiotemporal detection and analysis of exocytosis

ExoJ: an ImageJ2/Fiji plugin for automated spatiotemporal detection and analysis of exocytosis

Recent insights into the structure and function of Mitofusins in mitochondrial fusion

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