SNF1/AMPK/SnRK1 kinases, global regulators at the heart of energy control?

Polge, Cécile; Thomas, Martine

doi:10.1016/j.tplants.2006.11.005

Cited by 326 publications

(304 citation statements)

References 82 publications

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“…Yeast SNF1, mammalian AMPK, and plant SnRK1 are a group of Ser/Thr protein kinases that are conserved in all eukaryotes and have similar subunit compositions, subunit structures, and common kinase cascades (Hardie et al, 1998;Halford et al, 2003Halford et al, , 2004Hardie, 2007;Polge and Thomas, 2007;Baena-González and Sheen, 2008). In plants, SnRK1 is widely recognized to be involved in various physiological processes, including nutrient and energy sensing, global regulation of metabolism, control of the cell cycle, modulation of development, and response to abiotic or biotic stress (Baena-González et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Tomato SlSnRK1 Protein Interacts with and Phosphorylates βC1, a Pathogenesis Protein Encoded by a Geminivirus β-Satellite

et al. 2011

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The bC1 protein of tomato yellow leaf curl China b-satellite functions as a pathogenicity determinant. To better understand the molecular basis of bC1 in pathogenicity, a yeast two-hybrid screen of a tomato (Solanum lycopersicum) cDNA library was carried out using bC1 as bait. bC1 interacted with a tomato SUCROSE-NONFERMENTING1-related kinase designated as SlSnRK1. Their interaction was confirmed using a bimolecular fluorescence complementation assay in Nicotiana benthamiana cells. Plants overexpressing SnRK1 were delayed for symptom appearance and contained lower levels of viral and satellite DNA, while plants silenced for SnRK1 expression developed symptoms earlier and accumulated higher levels of viral DNA. In vitro kinase assays showed that bC1 is phosphorylated by SlSnRK1 mainly on serine at position 33 and threonine at position 78. Plants infected with bC1 mutants containing phosphorylation-mimic aspartate residues in place of serine-33 and/or threonine-78 displayed delayed and attenuated symptoms and accumulated lower levels of viral DNA, while plants infected with phosphorylation-negative alanine mutants contained higher levels of viral DNA. These results suggested that the SlSnRK1 protein attenuates geminivirus infection by interacting with and phosphorylating the bC1 protein.

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Section: Discussionmentioning

confidence: 99%

Tomato SlSnRK1 Protein Interacts with and Phosphorylates βC1, a Pathogenesis Protein Encoded by a Geminivirus β-Satellite

et al. 2011

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“…To deal with energy-depleting stress, eukaryotic organisms express orthologous signaling kinases, notably Snf1 in yeast, AMPK in mammals, and SnRK1 in plants (Polge and Thomas, 2007). Arabidopsis SnRK1s (KIN10 and KIN11) have high structural similarity to the catalytic a-subunits of yeast Snf1 and mammalian AMPK (Amodeo et al, 2007;Chen et al, 2009;Xiao et al, 2011) and play similar regulatory roles in stress-inducible gene expression (Baena-González et al, 2007;Jossier et al, 2009;Hardie, 2011).…”

Section: Snrk1 Activates Specific Stress-responsive Gene Expression Imentioning

confidence: 99%

“…Limited availability of sugar or energy in eukaryotic cells activates an evolutionarily conserved energy-sensing protein kinase (PK) known as Sucrose-nonfermentation1 (Snf1) in yeast, AMPK in mammals, and Snf1-related protein kinase1 (SnRK1) in plants (Polge and Thomas, 2007;Hardie, 2011). As sessile organisms, land plants endure continual environmental fluctuations that may abruptly deplete their energy stores.…”

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confidence: 99%

Regulatory Functions of SnRK1 in Stress-Responsive Gene Expression and in Plant Growth and Development

et al. 2012

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Sucrose-nonfermentation1-related protein kinase1 (SnRK1) is an evolutionarily conserved energy sensor protein that regulates gene expression in response to energy depletion in plants. Efforts to elucidate the functions and mechanisms of this protein kinase are hampered, however, by inherent growth defects of snrk1-null mutant plants. To overcome these limitations and study SnRK1 functions in vivo, we applied a method combining transient expression in leaf mesophyll protoplasts and stable expression in transgenic plants. We found that both rice (Oryza sativa) and Arabidopsis (Arabidopsis thaliana) SnRK1 activities critically influence stress-inducible gene expression and the induction of stress tolerance. Genetic, molecular, and chromatin immunoprecipitation analyses further revealed that the nuclear SnRK1 modulated target gene transcription in a submergencedependent manner. From early seedling development through late senescence, SnRK1 activities appeared to modulate developmental processes in the plants. Our findings offer insight into the regulatory functions of plant SnRK1 in stressresponsive gene regulation and in plant growth and development throughout the life cycle.

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“…These kinases show structural similarities to the SNF1 kinase (for SUCROSE NONFERMENTING1) in yeast and the AMP-DEPEN-DENT PROTEIN KINASE in mammals, which function as master regulators of the energy balance that is essential for survival under stress (Polge and Thomas, 2007). In Arabidopsis, the ASPARAGINE SYNTHETASE1 (ASN1) gene, which encodes the final step in Asn biosynthesis, was proposed to be a target of the KIN10/11 pathway that regulates the level of Asn (BaenaGonzá lez et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Heterodimers of the Arabidopsis Transcription Factors bZIP1 and bZIP53 Reprogram Amino Acid Metabolism during Low Energy Stress

et al. 2011

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Control of energy homeostasis is crucial for plant survival, particularly under biotic or abiotic stress conditions. Energy deprivation induces dramatic reprogramming of transcription, facilitating metabolic adjustment. An in-depth knowledge of the corresponding regulatory networks would provide opportunities for the development of biotechnological strategies. Low energy stress activates the Arabidopsis thaliana group S1 basic leucine zipper transcription factors bZIP1 and bZIP53 by transcriptional and posttranscriptional mechanisms. Gain-of-function approaches define these bZIPs as crucial transcriptional regulators in Pro, Asn, and branched-chain amino acid metabolism. Whereas chromatin immunoprecipitation analyses confirm the direct binding of bZIP1 and bZIP53 to promoters of key metabolic genes, such as ASPARAGINE SYNTHETASE1 and PROLINE DEHYDROGENASE, the G-box, C-box, or ACT motifs (ACTCAT) have been defined as regulatory cis-elements in the starvation response. bZIP1 and bZIP53 were shown to specifically heterodimerize with group C bZIPs. Although single loss-of-function mutants did not affect starvation-induced transcription, quadruple mutants of group S1 and C bZIPs displayed a significant impairment. We therefore propose that bZIP1 and bZIP53 transduce low energy signals by heterodimerization with members of the partially redundant C/S1 bZIP factor network to reprogram primary metabolism in the starvation response.

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SNF1/AMPK/SnRK1 kinases, global regulators at the heart of energy control?

Cited by 326 publications

References 82 publications

Tomato SlSnRK1 Protein Interacts with and Phosphorylates βC1, a Pathogenesis Protein Encoded by a Geminivirus β-Satellite

Tomato SlSnRK1 Protein Interacts with and Phosphorylates βC1, a Pathogenesis Protein Encoded by a Geminivirus β-Satellite

Regulatory Functions of SnRK1 in Stress-Responsive Gene Expression and in Plant Growth and Development

Heterodimers of the Arabidopsis Transcription Factors bZIP1 and bZIP53 Reprogram Amino Acid Metabolism during Low Energy Stress

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