SNHG1 lncRNA negatively regulates miR-199a-3p to enhance CDK7 expression and promote cell proliferation in prostate cancer

Li, Jianping; Zhang, Zhipeng; Li, Xiong; Guo, Caixia; Jiang, Tao; Zeng, Lilan; Ge, Li; Wang, Juan

doi:10.1016/j.bbrc.2017.03.169

Cited by 101 publications

(79 citation statements)

References 25 publications

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“…For example, lncRNA H19 repressed PCa metastasis by regulating miR‐675 (Zhu et al, ). lncRNA GAS5/miR‐103 axis, lncRNA SNHG1/miR‐199‐3p axis, and lncRNA XIST/miR‐23a axis were involved in PCa cell proliferation, metastasis, and apoptosis (Dong et al, ; Du et al, ; Li et al, ). In this study, we validated that miR‐543 was a target miRNA of HCG11 using luciferase reporter assay for the first time.…”

Section: Discussionmentioning

confidence: 99%

lncRNA HCG11 regulates cell progression by targeting miR‐543 and regulating AKT/mTOR pathway in prostate cancer

Wang

Dong

et al. 2019

Cell Biology International

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Prostate cancer (PCa) is a common cancer worldwide, which mostly occurs in males over the age of 50. Accumulating evidence have determined that long non-coding RNA/microRNA (lncRNA/miRNA) axis plays a critical role in cell progression of cancers, including PCa. However, the pathogenesis of PCa has not been fully indicated. In this study, quantitative real-time polymerase chain reaction was used to detect the expression of HCG11 and miR-543. Western blot was applied to measure the protein expression of proliferating cell nuclear antigen, cleavage-caspase 3 (cle-caspase 3), N-cadherin, E-cadherin, GAPDH, P-AKT, AKT, p-mTOR, and mTOR. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), transwell invasion, and transwell migration assay were used to detect cell proliferation, invasion, and migration, respectively. The function and mechanism of lncRNA HCG11 were confirmed in PCa cell and xenograft mice models. Luciferase assay indicated that miR-543 was a target miRNA of HCG11. Further investigation revealed that overexpression of HCG11 inhibited cell proliferation, invasion, and migration, whereas induced cell apoptosis by regulating miR-543 expression in vitro and in vivo. More than that, lncRNA HCG11 inhibited phosphoinositide-3 kinase/protein kinaseB (PI3K/AKT) signaling pathway to suppress PCa progression. Our data showed the overexpression of HGC11inhibited PI3K/AKT signaling pathway by downregulating miR-543 expression, resulting in the suppression of cell growth in PCa. This finding proved a new regulatory network in PCa and provided a novel therapeutic target of PCa.

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Section: Discussionmentioning

confidence: 99%

lncRNA HCG11 regulates cell progression by targeting miR‐543 and regulating AKT/mTOR pathway in prostate cancer

Wang

Dong

et al. 2019

Cell Biology International

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“…Resent reports proved that lots of lncRNA functioned as carcinogens or tumour suppressors in diverse tumours, including PCa . For example lncRNA SNHG1 promoted cell proliferation in prostate cancer . Besides, lncRNA MEG3 inhibited proliferation and metastasis of gastric cancer via p53 signalling pathway .…”

Section: Introductionmentioning

confidence: 99%

LncRNA MEG3 inhibits the progression of prostate cancer by modulating miR‐9‐5p/QKI‐5 axis

Huang

Chen

et al. 2018

J Cellular Molecular Medi

136

102

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This study was designed to detecting the influences of lncRNA MEG3 in prostate cancer. Aberrant lncRNAs expression profiles of prostate cancer were screened by microarray analysis. The qRT‐PCR and Western blot were employed to investigating the expression levels of lncRNA MEG3, miR‐9‐5p and QKI‐5. The luciferase reporter assay was utilized to testifying the interactions relationship among these molecules. Applying CCK‐8 assay, wound healing assay, transwell assay and flow cytometry in turn, the cell proliferation, migration and invasion abilities as well as apoptosis were measured respectively. LncRNA MEG3 was a down‐regulated lncRNA in prostate cancer tissues and cells and could inhibit the expression of miR‐9‐5p, whereas miR‐9‐5p down‐regulated QKI‐5 expression. Overexpressed MEG3 and QKI‐5 could decrease the abilities of proliferation, migration and invasion in prostate cancer cells effectively and increased the apoptosis rate. On the contrary, miR‐9‐5p mimics presented an opposite tendency in prostate cancer cells. Furthermore, MEG3 inhibited tumour growth and up‐regulated expression of QKI‐5 in vivo. LncRNA MEG3 was a down‐regulated lncRNA in prostate cancer and impacted the abilities of cell proliferation, migration and invasion, and cell apoptosis rate, this regulation relied on regulating miR‐9‐5p and its targeting gene QKI‐5.

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“…However, recent studies have shown that less than 2% of the human genome is coding genes, and over 90% of the genes are noncoding genes that play regulatory roles in most systems . Noncoding RNA (ncRNA) can regulate gene expression at different levels such as epigenetic modification, transcription and posttranscription . NcRNAs can be divided into short ncRNAs, midsize ncRNAs and lncRNAs according to the length of their nucleotides .…”

Section: Introductionmentioning

confidence: 99%

“…4 Noncoding RNA (ncRNA) can regulate gene expression at different levels such as epigenetic modification, transcription and posttranscription. [5][6][7] NcRNAs can be divided into short ncRNAs, midsize ncRNAs and lncRNAs according to the length of their nucleotides. 8 Their lengths are 50, 50-200, and more than 200 nucleotides, respectively.…”

Section: Introductionmentioning

confidence: 99%

Long non‐coding small nucleolar RNA host genes in digestive cancers

et al. 2019

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Although long noncoding RNAs (lncRNAs) do not have protein coding capacities, they are involved in the pathogenesis of many types of cancers, including hepatocellular carcinoma, cervical cancer, and gastric cancer. Notably, the roles of lncRNAs are vital in nearly every aspect of tumor biology. Long non‐coding small nucleolar RNA host genes (lnc‐SNHGs) are abnormally expressed in multiple cancers, including urologic neoplasms, respiratory tumors, and digestive cancers, and play vital roles in these cancers. These host genes could participate in tumorigenesis by regulating proliferation, migration, invasion and apoptosis of tumor cells. This review focuses on the overview of the roles that lnc‐SNHGs play in the formation and progression of digestive cancers.

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SNHG1 lncRNA negatively regulates miR-199a-3p to enhance CDK7 expression and promote cell proliferation in prostate cancer

Cited by 101 publications

References 25 publications

lncRNA HCG11 regulates cell progression by targeting miR‐543 and regulating AKT/mTOR pathway in prostate cancer

lncRNA HCG11 regulates cell progression by targeting miR‐543 and regulating AKT/mTOR pathway in prostate cancer

LncRNA MEG3 inhibits the progression of prostate cancer by modulating miR‐9‐5p/QKI‐5 axis

Long non‐coding small nucleolar RNA host genes in digestive cancers

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