SNHG16 lncRNAs are overexpressed and may be oncogenic in human gastric cancer by regulating cell cycle progression

Abstract

The small nucleolar RNA host gene 16 (SNHG16) has recently been shown to be a putative oncogene in gastric cancer (GC) and other cancer types, but how its four lncRNA variants are expressed in any physiological and pathological situation remains unknown. To investigate the expression and function of the four lncRNA varia nts of SNHG16, mainly the variant 1, in GC, we performed quantitative PCR to determine the RNA levels of the four variants in 60 GC tissue samples and several cell lines. We also studied how knocking down of SNHG16 with siRNA affected proliferation, apoptosis, cell cycle progression, as well as migration and invasion of GC cells. Our results showed that variants 1 and 4 were overexpressed in GC tissues compared with adjacent uninvolved tissues. Knockdown of the four variants, mainly the variant 1, enhanced apoptosis and inhibited cell cycle progression of a GC cell line by arresting the cells at t he G1 phase. These cellular effects were associated not only with decreased protein levels of c-Myc, PCNA, cyclins D, E, A, and B, as well as CDKs 2 and 6, but also with increased protein levels of the p21, p27, and p53. Knockdown of total SNHG16 lncRNAs also inhibited invasion and migration of the 2 GC cells in vitro. These results collectively suggest that SNHG16 may be oncogenic in GC by regulating cell cycle progression and may serve as a GC biomarker.

A Novel N7-Methylguanosine-Related lncRNA Signature Predicts the Prognosis of Colon Adenocarcinoma Patients

“…lncRNA ZEB1-AS1Positive Reciprocal Feedback of and α Contributes to Hypoxia-Promoted Tumorigenesis and Metastasis of Pancreatic Cancer [18]. In human gastric cancer, SNHG16 lncRNAs are overexpressed and may be carcinogenic via in uencing cell cycle progression [19]. Long noncoding RNA LINC02474 affects colorectal cancer metastasis and apoptosis by inhibiting GZMB expression [20].…”

Roles of long non‑coding RNA SNHG16 in human digestive system cancer (Review)

“…The expression of SNHG16 is significantly associated with the depth of infiltration, lymph node metastasis, TNM stage, histological differentiation and PTBP1 expression of GC ( 169 , 170 ). Knockdown of SNHG16 can significantly suppress the migration, invasion and arrest of cells in the G 1 phase, and can decrease c-Myc expression, and affect the formation of the p27/cyclin D1/CDK6, p53/cyclin E1 and cyclin A2/CDK2 complexes ( 169 – 171 ). A number of patients with GC develop 5-fluorouracil (5-FU) resistance, showing a higher vulnerability than parental GC cells.…”