SNHG25 promotes colorectal cancer metastasis by regulating MMP2

Abstract

LncRNA has been shown to play an important role in tumors, but the functions of most lncRNAs in colorectal cancer is not clear. By analyzing the transcriptome data of tumor tissues and adjacent tissues, we identified the lncRNA profiles that were abnormally expressed in colorectal cancer and selected the abnormally highly expressed lncRNA SNHG25 for further study. The functional assays showed that after knocking down SNHG25, the metastatic ability of colorectal cancer cells was significantly reduced. Western blot and immunofluorescence assays showed that inhibiting SNHG25 would affect the expression of Vimentin and E-Cadherin. In terms of mechanism, the results of RNA pull down assays, RNA immunoprecipitation (RIP) assays and dual luciferase reporter assays showed that SNHG25 could promote MMP2 expression by adsorbing miR-296-3p. In addition, chromatin immunoprecipitation (ChIP) assays and promoter luciferase reporter assays revealed that PAX5 could activate the transcription of SNHG25 in colorectal cancer cells. Our study proved that SNHG25 acts a pro-metastasis role in colorectal cancer, enriching the theory of the functions of lncRNA in colorectal cancer.

Banxia-Shengjiang drug pair inhibits gastric cancer development and progression by improving body immunity

“…Secondly, according to the results of clinical correlation analysis, the expression of PTGS2, MMP9, MMP2 and CXCR4 genes in gastric cancer is higher than that in normal gastric tissues, and the expression of PPARG gene in gastric cancer is lower. [61–65] This indicates that the expression of the above genes causes obvious abnormal changes in cancer cells and tissues, and affects the growth status and proliferation rate of tumors. Furthermore, the correlation between hub genes and immune infiltration, gene mutation and methylation repair system will be analyzed to provide theoretical support for further precision treatment.…”