SNHG9 promotes Hepatoblastoma Tumorigenesis via miR-23a-5p/Wnt3a Axis

Feng, Sun; Bhandari, Rajeev; Liu, Ya; Bian, Zhixuan; Pan, Qiuhui; Zhu, Jun; Sewi, Mao; Zhen, Ni; Wang, Jing; Sun, Fenyong; Ma, Ji; Bhandari, Ramesh Singh

doi:10.7150/jca.60748

Cited by 12 publications

(5 citation statements)

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“…In lung cancer, SNHG9 played a role in cisplatin resistance by promoting the CAPRIN1 gene [ 46 ], and the knockdown of SNHG9 decreased cell proliferation and invasion [ 47 ]. With regard to liver cancers, it was shown that SNHG9 promoted hepatoblastoma tumorigenesis via miR-23a-5p / Wnt3a axis [ 48 ]. Moreover, decreasing SNHG9 reduced methylation of the GSTP1 gene and inhibited liver cancer cell proliferation, migration and invasion [ 49 ].…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic Analyses Reveal Long Non-Coding RNA in Peripheral Blood Mononuclear Cells as a Novel Biomarker for Diagnosis and Prognosis of Hepatocellular Carcinoma

Kunadirek

Pinjaroen

Nookaew

et al. 2022

IJMS

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Novel biomarkers are highly required for the diagnosis and predicting prognosis of hepatocellular carcinoma (HCC). In this study, we investigated the profiles of long non-coding RNAs (lncRNAs) obtained from the peripheral blood mononuclear cells (PBMCs) of patients with HCC and PBMCs from a co-culture model using transcriptomic analysis. The differentially expressed lncRNAs (DElncRNAs) were then characterized and integrated as cancer-induced lncRNAs. Among them, three up-regulating DElncRNAs including MIR4435-2HG, SNHG9 and lnc-LCP2-1 and one down-regulating, lnc-POLD3-2, were identified. The functional analysis showed that these enriched lncRNAs were mainly associated with carcinogenesis and immune responses. Following further validation in PBMCs samples (100 HBV-related HCC, 100 chronic hepatitis B and 100 healthy controls), MIR4435-2HG, lnc-POLD3-2 and their combination were revealed to be sensitive biomarkers in discriminating HCC from non-HCC (AUROC = 0.78, 0.80, and 0.87, respectively), particularly among individuals with normal serum alpha-fetoprotein levels. Additionally, high circulating SNHG9 expression was shown to be an independent prognostic factor of overall survival in patients with HCC. These results indicate that determining these lncRNAs in PBMCs could serve as novel diagnostic and prognostic biomarkers for HBV-related HCC.

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Section: Discussionmentioning

confidence: 99%

Transcriptomic Analyses Reveal Long Non-Coding RNA in Peripheral Blood Mononuclear Cells as a Novel Biomarker for Diagnosis and Prognosis of Hepatocellular Carcinoma

Kunadirek

Pinjaroen

Nookaew

et al. 2022

IJMS

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show abstract

“…Prior evidence has suggested that miR-23a-5p exhibits oncogenic activity in bladder cancer (Li et al 2018) and renal cell carcinoma (Quan et al 2017), and it can additionally influence tumorigenesis and disease progression through interactions with specific lncRNAs or mRNAs. In glioblastoma, for example, the lncRNATPT1-AS1 can promote growth activity through the sequestration of miR-23a-5p (Gao et al 2021), whereas the lncRNA FLVCR1-AS1 can enhance cervical cancer cell malignancy via the miR-23a-5p/SLC7A11 axis (Zhou et al 2022) and hepatoblastoma progression is at least partially driven by the SNHG9/miR-23a-5p/Wnt3a signaling pathway (Feng et al 2021). Besides affecting tumor progression, miR-23a-5p could modulate the innate host defense by promoting mycobacteria survival and inhibiting the activation of autophagy against Mycobacterium tuberculosis (M.tb.)…”

Section: Discussionmentioning

confidence: 99%

The linear ANRIL transcript P14AS regulates the NF-κB signaling to promote colon cancer progression

Ma,

2023

Mol Med

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Background The linear long non-coding RNA P14AS has previously been reported to be dysregulated in colon cancer, but the mechanistic role that P14AS plays in colon cancer progression has yet to be clarified. Accordingly, this study was developed to explore the regulatory functions of ANRIL linear transcript-P14AS in cancer. Methods The expression of P14AS, ANRIL, miR-23a-5p and their target genes were detected by quantitative real-time polymerase chain reaction (qRT-PCR) and western blot. Cell supernatants of IL6 and IL8 were measured by Enzyme linked immunosorbent (ELISA) assay. Dual-luciferase reporter assays, RNA immunoprecipitation, or pull-down assays were used to confirm the target association between miR-23a-5p and P14AS or UBE2D3. Cell proliferation and chemosensitivity of NF-κB inhibitor BAY 11-7085 were evaluated by cell counting kit 8 (CCK8). Results When P14AS was overexpressed in colon cancer cell lines, enhanced TNF-NF-κB signaling pathway activity was observed together with increases in IL6 and IL8 expression. The Pita, miRanda, and RNA hybrid databases revealed the ability of miR-23a-5p to interact with P14AS, while UBE2D3 was further identified as a miR-23a-5p target gene. The results of dual-luciferase reporter, RNA pull-down, and RNA immunoprecipitation experiments confirmed these direct interactions among P14AS/miR-23a-5p/UBE2D3. The degradation of IκBa mediated by UBE2D3 may contribute to enhanced NF-κB signaling in these cells. Moreover, the beneficial impact of P14AS on colon cancer cell growth was eliminated when cells were treated with miR-23a-5p inhibitors or UBE2D3 was silenced. As such, these findings strongly supported a role for the UBE2D3/IκBa/NF-κB signaling axis as a mediator of the ability of P14AS to promote colon cancer progression. Conclusions These data suggested a mechanism through which the linear ANRIL transcript P14AS can promote inflammation and colon cancer progression through the sequestration of miR-23a-5p and the modulation of NF-κB signaling activity, thus highlighting P14AS as a promising target for therapeutic intervention efforts.

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“…It has been demonstrated that SNHG9 exerts its biological effects by binding directly to miRNAs. For instance, SNHG9 promotes carcinogenesis in hepatoblastoma by downregulating miR-23a-5p and upregulating Wnt3a ( Feng et al, 2021 ). SNHG9, when increased in glioblastoma stem cells (GSCs), functions as a ceRNA for miR-326 and promotes the development of GSCs ( Wang et al, 2022 ).…”

Section: Discussionmentioning

confidence: 99%

Long non-coding RNA SNHG9 regulates viral replication in rhabdomyosarcoma cells infected with enterovirus D68 via miR-150-5p/c-Fos axis

et al. 2023

Front. Microbiol.

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BackgroundThe Enterovirus D68 (EV-D68) epidemic has increased knowledge of the virus as a pathogen capable of causing serious respiratory and neurological illnesses. It has been shown that long noncoding RNAs (lncRNAs) regulate viral replication and infection via multiple mechanisms or signaling pathways. However, the precise function of lncRNAs in EV-D68 infection remains unknown.MethodsThe differential expression profiles of lncRNA in EV-D68-infected and uninfected rhabdomyosarcoma (RD) cells were studied using high-throughput sequencing technology. The knockdown through small interfering RNA (siRNA) and overexpression of lncRNA SNHG9 (small ribonucleic acid host gene 9) were applied to investigate how lncRNA SNHG9 regulates EV-D68 propagation. The targeted interactions of lncRNA SNHG9 with miR-150-5p and miR-150-5p with c-Fos were validated using dual luciferase reporter system. LncRNA SNHG9 knockdown and miR-150-5p inhibitor were co-transfected with RD cells. QRT-PCR and western blot were used to detect RNA and protein levels, of c-Fos and VP1, respectively. Median tissue culture infectious dose (TCID50) was applied to detect viral titers.ResultsThe results demonstrated that a total of 375 lncRNAs were highly dysregulated in the EV-D68 infection model. In the EV-D68 infection model, lncRNA SNHG9 and c-Fos were increased in EV-D68-infected RD cells. However, the expression level of miR-150-5p was downregulated. In addition, overexpression of SNHG9 in RD cells resulted in decreased viral replication levels and viral titers following infection with EV-D68, and further experiments revealed that overexpression of SNHG9 inhibited the viral replication by targeting increased miR-150-5p binding and significantly increased c-Fos expression in RD cells.ConclusionOur findings indicate that the SNHG9/miR-150-5p/c-Fos axis influences EV-D68 replication in host cells and that SNHG9 may be a possible target for anti-EV-D68 infection therapies.

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SNHG9 promotes Hepatoblastoma Tumorigenesis via miR-23a-5p/Wnt3a Axis

Cited by 12 publications

References 50 publications

Transcriptomic Analyses Reveal Long Non-Coding RNA in Peripheral Blood Mononuclear Cells as a Novel Biomarker for Diagnosis and Prognosis of Hepatocellular Carcinoma

Transcriptomic Analyses Reveal Long Non-Coding RNA in Peripheral Blood Mononuclear Cells as a Novel Biomarker for Diagnosis and Prognosis of Hepatocellular Carcinoma

The linear ANRIL transcript P14AS regulates the NF-κB signaling to promote colon cancer progression

Long non-coding RNA SNHG9 regulates viral replication in rhabdomyosarcoma cells infected with enterovirus D68 via miR-150-5p/c-Fos axis

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