Sniffer cells for the detection of neural Angiotensin II in vitro

Farmer, George; Amune, Anna; Bachelor, Martha; Duong, Phong; Yuan, Joseph P.; Cunningham, J. Thomas

doi:10.1038/s41598-019-45262-4

Cited by 10 publications

(5 citation statements)

References 29 publications

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“…It was recently demonstrated that SFO neuronal activation is able to elicit release of ANG II into other brain areas 42 . Both PVN and ARC, the key brain regions for metabolic control and food regulation, receive direct projections from the SFO and are highly responsive to ANG II via AT 1 Rs 6–8 .…”

Section: Discussionmentioning

confidence: 99%

“…It was recently demonstrated that SFO neuronal activation is able to elicit release of ANG II into other brain areas. 42 Both PVN and ARC, the key brain regions for metabolic control and food regulation, receive direct projections from the SFO and are highly responsive to ANG II via AT 1 Rs. [6][7][8] We also found a significant increase in the Agtr1a mRNA expression in the PVN after 48 h of fasting in rats, confirming previous findings that indicated increased AT 1 R binding after 2 weeks of severe food restriction.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Fasting increases circulating angiotensin levels and brain Agtr1a expression in male rats

Paes‐Leme

Monteiro

Gholami

et al. 2023

J Neuroendocrinology

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Besides being recognised for involvement in cardiovascular control and hydromineral balance, the renin‐angiotensin system (RAS) has also been associated with the neuroendocrine control of energy balance. One of the main brain sites for angiotensin II (ANG II)/type 1 receptor (AT1R) signalling is the subfornical organ (SFO), a circumventricular organ related to the control of autonomic functions, motivated behaviours and energy metabolism. Thus, we hypothesised that circulating ANG II may act on the SFO AT1R receptors to integrate metabolic and hydromineral balance. We evaluated whether food deprivation can modulate systemic RAS activity and Agrt1a brain expression, and if ANG II/AT1R signalling influences the hypothalamic expression of mRNAs encoding neuropeptides and food and water ingestion in fed and fasted Wistar rats. We found a significant increase in both ANG I and ANG II plasma levels after 24 and 48 hours of fasting. Expression of Agrt1a mRNA in the SFO and paraventricular nucleus (PVN) also increased after food deprivation for 48 h. Treatment of fasted rats with low doses of losartan in drinking water attenuated the decrease in glycemia and meal‐associated water intake without changing the expression in PVN or arcuate nucleus of mRNAs encoding selected neuropeptides related to energy homeostasis control. These findings point to a possible role of peripheral ANG II/SFO‐AT1R signalling in the control of re‐feeding‐induced thirst. On the other hand, i.c.v. losartan treatment decreased food and water intake over dark time in fed but not in fasted rats.This article is protected by copyright. All rights reserved.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Fasting increases circulating angiotensin levels and brain Agtr1a expression in male rats

Paes‐Leme

Monteiro

Gholami

et al. 2023

J Neuroendocrinology

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“…Most of the available techniques used to detect and measure the release of peptides or neurotransmitters from biological preparations, such as microdialysis, high-pressure liquid chromatography, or electrochemical techniques, offer great levels of sensitivity but lack the temporal and spatial resolution required to study quantal release from single cells [69, 70]. Sniffer cells can be used as biosensors to detect the release of peptides or neurotransmitters with optimal sensitivity and spatiotemporal resolution.…”

Section: Genetic Labelling Methodsmentioning

confidence: 99%

Imaging the Hypothalamo-Neurohypophysial System

Bárez-López¹,

Scanlon²,

Murphy³

et al. 2021

Neuroendocrinology

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The hypothalamo-neurohypophysial system (HNS) is a brain peptidergic neurosecretory apparatus which is composed of arginine vasopressin (AVP) and oxytocin (OXT) magnocellular neurones and their neuronal processes in the posterior pituitary (PP). In response to specific stimuli, AVP and OXT are secreted into the systemic circulation at the neurovascular interface of the PP, where they act as hormones, but they can also behave as neurotransmitters when released at the somatodendritic compartment or by axon collaterals to other brain regions. Because these peptides are crucial for several physiological processes, including fluid homoeostasis and reproduction, it is of great importance to map the HNS connectome in its entirety in order to understand its functions. In recent years, advances in imaging technologies have provided considerable new information about the HNS. These approaches include the use of reporter proteins under the control of specific promoters, viral tracers, brain-clearing methods, genetically encoded indicators, sniffer cells, mass spectrometry imaging, and spatially resolved transcriptomics. In this review, we illustrate how these latest approaches have enhanced our understanding of the structure and function of the HNS and how they might contribute further in the coming years.

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“…This supports the idea that the SFO senses changes in [Na + ] to regulate sodium appetite. In addition to hormonal actions, there is some evidence that ANGII acts as a neurotransmitter [ 116 , 117 , 118 , 119 , 120 ] and is therefore capable of stimulating AT1R in areas within the blood–brain barrier. The SFO has dense angiotensin-sensitive projections to the BNST [ 52 , 121 ] and PVN [ 117 , 118 ].…”

Section: Neural and Hormonal Control Of Sodium Intakementioning

confidence: 99%

Sodium Intake and Disease: Another Relationship to Consider

et al. 2023

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Sodium (Na+) is crucial for numerous homeostatic processes in the body and, consequentially, its levels are tightly regulated by multiple organ systems. Sodium is acquired from the diet, commonly in the form of NaCl (table salt), and substances that contain sodium taste salty and are innately palatable at concentrations that are advantageous to physiological homeostasis. The importance of sodium homeostasis is reflected by sodium appetite, an “all-hands-on-deck” response involving the brain, multiple peripheral organ systems, and endocrine factors, to increase sodium intake and replenish sodium levels in times of depletion. Visceral sensory information and endocrine signals are integrated by the brain to regulate sodium intake. Dysregulation of the systems involved can lead to sodium overconsumption, which numerous studies have considered causal for the development of diseases, such as hypertension. The purpose here is to consider the inverse—how disease impacts sodium intake, with a focus on stress-related and cardiometabolic diseases. Our proposition is that such diseases contribute to an increase in sodium intake, potentially eliciting a vicious cycle toward disease exacerbation. First, we describe the mechanism(s) that regulate each of these processes independently. Then, we highlight the points of overlap and integration of these processes. We propose that the analogous neural circuitry involved in regulating sodium intake and blood pressure, at least in part, underlies the reciprocal relationship between neural control of these functions. Finally, we conclude with a discussion on how stress-related and cardiometabolic diseases influence these circuitries to alter the consumption of sodium.

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Sniffer cells for the detection of neural Angiotensin II in vitro

Cited by 10 publications

References 29 publications

Fasting increases circulating angiotensin levels and brain Agtr1a expression in male rats

Fasting increases circulating angiotensin levels and brain Agtr1a expression in male rats

Imaging the Hypothalamo-Neurohypophysial System

Sodium Intake and Disease: Another Relationship to Consider

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