SNOM and AFM microscopy techniques to study the effect of non‐ionizing radiation on the morphological and biochemical properties of human keratinocytes cell line (HaCaT)

Rieti, Sabrina; Manni, Vanessa; Lisi, Antonella; Giuliani, Livio; Sacco, D; D’Emilia, Enrico; Cricenti, A.; Generosi, R.; Luce, M.; Grimaldi, Settimio

doi:10.1111/j.1365-2818.2004.01279.x

Cited by 48 publications

(38 citation statements)

References 51 publications

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“…This interaction may contribute to the change of cell size, shape, and orientation observed in our and others experiment (33,34). In addition, alignment of actin filaments in a magnetic field has been demonstrated, indicating that the condensed counter-ion system of actin filaments is able to convert magnetic field energy into mechanical movement (35).…”

Section: Discussionmentioning

confidence: 82%

Static magnetic fields affect cell size, shape, orientation, and membrane surface of human glioblastoma cells, as demonstrated by electron, optic, and atomic force microscopy

et al. 2006

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Background: It is common knowledge that static magnetic fields (SMF) do not interact with living cells; thus, fewer studies of SMF compared with variable magnetic fields are carried out. However, evidence demonstrated that SMF affect cellular structures. To investigate the effect of exposure to increasing doses of SMF on cell morphology, human glioblastoma cells were exposed to SMF ranging between 80 and 3,000 G (8 and 300 mT). Methods: Cell morphology of human glioblastoma cells, derived from a primary culture, was studied by electron and optic microscopy. FITC-phalloidin staining of actin filaments was also investigated. Finally, cell surface structure changes were detected by atomic force microscopy.Results: Scanning electron microscopy demonstrated a dose-dependent cell shape modification, progressive cell detachment, loss of the long villi, and appearance of membrane roughness and blebs. FITC-phalloidin staining confirmed the villi retention and cell dimension decrease. At

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Section: Discussionmentioning

confidence: 82%

Static magnetic fields affect cell size, shape, orientation, and membrane surface of human glioblastoma cells, as demonstrated by electron, optic, and atomic force microscopy

et al. 2006

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“…While control cells exhibited nicely spread globular shapes, treated cells evolved toward elongated forms involving a cell surface reduction of more than twofold; this decrease found expression in a shrinking and a lack of adhesion characterizing apoptotic phenomena. Other studies (Rieti et al 2004;Deng et al 2009) showed an equivalent behavioral response to various external stimuli, fitting with a "round to elongated" cell transition. Reich et al (2007) related alterations of the keratinocyte morphology after ultraviolet B (UVB) irradiation, impairment of contact with adjacent keratinocytes, and detachment of cells from the supporting glass surface.…”

Section: Discussionmentioning

confidence: 94%

Morphological damages of a glyphosate-treated human keratinocyte cell line revealed by a micro- to nanoscale microscopic investigation

et al. 2009

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Among the molecules to which the human skin is exposed, glyphosate is used as an herbicide. Glyphosate has been shown to induce in vitro cutaneous cytotoxic effects, concomitant with oxidative disorders. In this following study, we focused on dynamic events of the loss of HaCaT cell integrity appearing after a glyphosate treatment. In these conditions, we showed that glyphosate is able to disrupt HaCaT cells and to induce intracellular oxidative cascade. In this aim, we optimized the conditions of cell treatment playing on exposure time (from 24 h to 30 min), which directly modify the cell viability profile (glyphosate 50% inhibition concentration from 28 to 53 mM) and allow to track cells along the treatment as an "induction and visualization" process. The combination of atomic force and fluorescence microscopic approaches offered opportunities to lead in parallel an investigation of the membrane surface and of the intracellular disorders, through cytoskeleton, nuclear, and oxidative stress marker targeting. The originality of our approach relies on monitoring all events derived from oxidative stress in process and performed by simultaneous cytotoxic induction and nanoscale cell visualization. We revealed a transition from spread and globular to elongated cell morphology, with a drastic cell size reduction, after a dose- and time-dependent glyphosate treatment; a redistribution of cell surface protrusions was also pointed out. All these membrane damages, added to observations of disorganized cytoskeleton, condensed chromatin, and overproduction of oxidative reactive species, lead us to conclude that glyphosate acts in induction of apoptotic process.

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“…de-arrange the actin network [147,149,153,168,178,182]. Exposure to SMFs of varying intensities and types has been shown to cause cell type-, age-and time-related modifications to cell shape and to cell surface morphology [146,148,152,167,180,[182][183][184][185][186][187] and the redistribution of plasma membrane proteins, receptors [188] and sugar residues [149,153,189]. One particular modification of the cell surface, observed in various cell lines exposed to fields of varying type and intensity, is the presence of many lamellar microvilli and blebs well before the distortion of the cell shape, which was found at periods of exposure longer than 24 h [153,179,186].…”

Section: Cell Functions and Structuresmentioning

confidence: 99%

Phagocytosis of dying cells: influence of smoking and static magnetic fields

Dini

2010

Apoptosis

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It is becoming evident that failure in the removal of dying cells causes and/or promotes the onset of chronic diseases. Impairment of phagocytosis of apoptotic cells can be due not only to genetic or molecular malfunctioning but also to external/environmental factors. Two of these environmental factors have been recently reported to down regulate the clearance of apoptotic cells: cigarette smoke and static magnetic fields. Cigarette smoke contains highly reactive carbonyls that modify proteins which directly/indirectly affects cellular function. Human macrophages interacting with carbonyl or cigarette smoke modified extracellular matrix (ECM) proteins dramatically down regulated their ability to phagocytose apoptotic neutrophils. It was postulated that changes in the ECM environment as a result of cigarette smoke affect the ability of macrophages to remove apoptotic cells. This decreased phagocytic activity was as a result of sequestration of receptors involved in the uptake of apoptotic cells towards that of recognition of carbonyl adducts on the modified ECM proteins leading to increased macrophage adhesion. Downregulation of the phagocytosis of apoptotic cells was also described when performed in presence of static magnetic fields (SMFs) of moderate intensity. SMFs have been reported to perturb distribution of membrane proteins and glycoproteins, receptors, cytoskeleton and trans-membrane fluxes of different ions, especially calcium [Ca(2+)]i, that in turn, interfere with many different physiological activities, including phagocytosis. The effects of cigarette smoke and SMF on the phagocytosis of dying cells will be here discussed.

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SNOM and AFM microscopy techniques to study the effect of non‐ionizing radiation on the morphological and biochemical properties of human keratinocytes cell line (HaCaT)

Cited by 48 publications

References 51 publications

Static magnetic fields affect cell size, shape, orientation, and membrane surface of human glioblastoma cells, as demonstrated by electron, optic, and atomic force microscopy

Static magnetic fields affect cell size, shape, orientation, and membrane surface of human glioblastoma cells, as demonstrated by electron, optic, and atomic force microscopy

Morphological damages of a glyphosate-treated human keratinocyte cell line revealed by a micro- to nanoscale microscopic investigation

Phagocytosis of dying cells: influence of smoking and static magnetic fields

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