SNP marker-based genetic mapping of rust resistance gene in the vegetable cowpea landrace ZN016

Abstract

Cowpea rust, caused by Uromyces vignae, is one of the most destructive foliar diseases in cowpea [V. unguiculata. (L.) Walp]. Deployment of rust-resistant cultivars is the most environment-friendly and effective way to curb this disease. For this purpose, a fundamental step is to map the rust resistance genes in elite germplasm lines. In the current study, one major and two minor QTLs conferring rust resistance were detected in a 119-line recombinant inbred line (RIL) population derived from a cross between the rust resistant line "ZN016" and a susceptible cultivar "Zhijiang 282", using the state-ofthe-art cowpea iSelect Consortium Array.The major QTL, designated as Ruv1, was mapped to a 12.48 cM interval between the SNP markers 2_01772 and 2_03292 on LG09, which explained 34.8% of the phenotypic variation. The minor QTLs, designated as Ruv2 and Ruv3, were mapped to a 7.01 cM interval on LG7 and a 6.19 cM interval on LG8, which accounted for 13.4% and 11.9% of the phenotypic variation, respectively. This study demonstrates the usefulness of the Consortium SNP Array on complex trait mapping and solidifies the basis for marker-assisted breeding of new cowpea varieties with improved rust resistance.

Identification of New Sources of Rust Resistance in Cowpea (Vigna Unguiculata L. Walp) Germplasm Using Simple Sequence Repeat Markers

“…The difference in amplicon size may be due to differences in the genetic composition of the cowpea genotypes and the differences in inheritance of rust resistance in the genotypes (Uma et al, 2011). Rust resistance in cowpea is generally controlled by recessive genes, dominance genes with additive effects, or polygenes located at different loci on the genome (Rangaiah, 1997; Salimath, 2004;Wu et al, 2018). In line with the current work, the implication is that the cowpea genotypes may exhibit different resistance to cowpea rust isolate(s) as compared to rust isolate(s) infecting the genotypes from which VuUGM02, VuUGM08 and VuUGM19 SSR primers were developed (Uma et al, 2016).…”

Crop Landraces and Indigenous Varieties: A Valuable Source of Genes for Plant Breeding

“…Landraces have also been used as parents for RILs’ development, especially regarding resistance to various biotic stresses and related genetic loci [ 62 , 63 ]. Landraces that served as parents to form RIL or multi-parent advanced generation inter-cross (MAGIC) populations with resistance to pests and diseases are presented in Table 1 .…”

DNA Fingerprinting of Vegetable Soybean Cultivar ‘Zhexian No.9’ Using 101 New Developed HRM-Based SNP Markers

“…However, along with the development of SNP (single nucleotide polymorphism) markers and the detection technologies, the third-generation molecular markers show overwhelming advantages than all of the previously mentioned markers. SNP markers have been proved to be the most stable, abundant, easy to be high-throughput analyzed and most promising molecular marker (Gupta et al 2001;Hayashi et al 2004), which were used in germplasm identification and breeding programs (Wu et al 2014;Wu et al 2018).…”