SNPs Associated with Foliar Phylloxera Tolerance in Hybrid Grape Populations Carrying Introgression from Muscadinia

Abstract

Leaf-feeding phylloxera decreases the photosynthetic activity of a grape plant, leading to decreasing number of fruit buds. In addition, phylloxera larvae emerging from the leaf galls may colonize the roots, negatively affecting the growth of the grape plant. In this study, we evaluated host tolerance of three grapevine hybrid populations obtained from crossing of the same maternal grapevine M. no. 31-77-10 with interspecific hybrids carrying introgressions from Muscadinia and other North American Vitis species against leaf-feeding grape phylloxera. Combining genotyping data of the populations obtained with 12,734 SNPs and their resistance phenotypes evaluated in the laboratory experiment, we performed an association study. As the result of GWAS, nine SNPs with the lowest significant p-values were discovered in the whole sample of 139 hybrids as associated with variation of the scores ‘the percentage of infested leaves’ and ‘intensity of gall formation’. Three of the SNPs on LG 7 were located in the same chromosome interval where a major QTL (RDV6) for root phylloxera resistance was reported from Muscadine background. Two SNPs on LG 8 were detected within the gene, encoding E3 ubiquitin-protein ligase UPL4 involved in apoptosis. SNPs detected on LG 13 and LG 18 may overlap with the previously reported QTLs for phylloxera resistance inherited from V. cinerea.

Multi-Environment Genome-Wide Association Studies of Yield Traits in Common Bean (Phaseolus vulgaris L.) × Tepary Bean (P. acutifolius A. Gray) Interspecific Advanced Lines in Humid and Dry Colombian Caribbean Subregions

“…Polygenic, or even omnigenic [73], adaptation is also not rare because many standing genetic variants with modest effect sizes are expected to underlie early stages of selection favoring complex abiotic tolerance [35] in interspecific genomic backgrounds. Numerous examples have reported polygenic architectures as part of hybrid breeding schemes, as follows: phytochemical, morphological, and growth traits in hybrid Populus genotypes [74], biotic resistance in hybrid grapes [75], morphological and yield-related traits in hybrid oil palm [76], agronomic traits in hybrid cotton [77], the flowering time in Amaranthus [78], phenology-related traits in blueberries [79], and the grain yield and other yield-related traits in hybrid wheat under drought stress [80].…”

Chemical Profile of “Folla Redonda” Grape; Differentiation with Traditional Winemaking Grapes

“…However, this situation of splendor for the vineyards has not always been like this. Towards the end of 1870, a plague of vine diseases, such as phylloxera, penetrated the Iberian Peninsula through Oporto [2]. Phylloxera is a parasitic insect of the Phylloxeridae family that infects the roots of grapevine plants, where it feeds on the plant's sap, weakening and eventually killing the roots and hindering vine growth [3,4].…”

“…This significantly affected France, Italy, Spain, and Germany, leading to the destruction of vast swathes of vineyards and a significant impact on wine production throughout these regions [5]. 2 During this period, it was discovered that American vines such as Vitis labrusca and Vitis riparia were resistant to this plague. This led to these species being used as rootstocks on which many European vines were grafted, thus allowing the recovery of wine at the end of the 19th century [6,7].…”

Multi-environment Genome Wide Association Studies of Yield Traits in Common Bean (Phaseolus vulgaris L.) × Tepary Bean (P. acutifolius A. Gray) Interspecific Advanced Lines at the Humid and Dry Colombian Caribbean Subregions

“…Several plant breeders have explored the use of interspecific panel of genotypes using GWAS to improve the reconstruction of the genetic basis of quantitative traits. In the tree context, some authors have used hybrids genotypes in GWAS for phytochemical, morphological and growth traits in hybrid Populus (Bresadola et al, 2019), and biotic resistance in hybrid grape (Vasylyk et al, 2022). In oil palms, authors as (Osorio-Guarín et al, 2019) have implemented hybrid Elaeis in GWAS for morphological and yield-related traits.…”