SnRK1 phosphorylation of FUSCA3 positively regulates embryogenesis, seed yield, and plant growth at high temperature in Arabidopsis

Chan, Aaron; Carianopol, Carina Steliana; Tsai, Allen Yi‐Lun; Varatharajah, Kresanth; Chiu, Rex Shun; Gazzarrini, Sonia

doi:10.1093/jxb/erx233

Cited by 42 publications

(25 citation statements)

References 55 publications

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“…, 2004; Suzuki & McCarty, 2008) and FUS3 is regulated by ABA signalling through SnRK1 phosphorylation (Chan et al . , 2017). To explore the functional mechanism of PdFUS3 in regulating ABA homeostasis in poplar root, promoter trans ‐activation assays were conducted in poplar plantlet by transient expression.…”

Section: Resultsmentioning

confidence: 99%

Root‐specific NF‐Y family transcription factor, PdNF‐YB21, positively regulates root growth and drought resistance by abscisic acid‐mediated indoylacetic acid transport in Populus

et al. 2020

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Summary Root growth control plays an important role in plant adaptation to drought stress, but the underlying molecular mechanisms of this control remain largely elusive. Here, a root‐specific nuclear factor Y (NF‐Y) transcription factor PdNF‐YB21 was isolated from Populus. The functional mechanism of PdNF‐YB21 was characterised by various morphological, physiological, molecular, biochemical and spectroscopy techniques. Overexpression of PdNF‐YB21 in poplar promoted root growth with highly lignified and enlarged xylem vessels, resulting in increased drought resistance. By contrast, CRISPR/Cas9‐mediated poplar mutant nf‐yb21 exhibited reduced root growth and drought resistance. PdNF‐YB21 interacted with PdFUSCA3 (PdFUS3), a B3 domain transcription factor. PdFUS3 directly activated the promoter of the abscisic acid (ABA) synthesis key gene PdNCED3, resulting in a significant increase in root ABA content in poplars subjected to water deficit. Coexpression of poplar NF‐YB21 and FUS3 significantly enhanced the expression of PdNCED3. Furthermore, ABA promoted indoylacetic acid transport in root tips, which ultimately increased root growth and drought resistance. Taken together, our data indicate that NF‐YB21–FUS3‐NCED3 functions as an important avenue in auxin‐regulated poplar root growth in response to drought.

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Section: Resultsmentioning

confidence: 99%

Root‐specific NF‐Y family transcription factor, PdNF‐YB21, positively regulates root growth and drought resistance by abscisic acid‐mediated indoylacetic acid transport in Populus

et al. 2020

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“…It is delicate that the photosynthetically fixed carbon per se switches on FA synthesis so as to flow into storage. Notably, FUS3 is a substrate of SnRK1 as well; however, in contrast to WRI1, phosphorylation by KIN10 is conducive to its protein stability and TF activity (Tsai and Gazzarrini, 2012;Chan et al, 2016).…”

Section: Signaling Pathwaysmentioning

confidence: 99%

Plant Unsaturated Fatty Acids: Biosynthesis and Regulation

et al. 2020

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In most plants, major unsaturated fatty acids (UFAs) are three C18 species, namely, oleic (18:1), linoleic (18:2), and α-linolenic (18:3) acids. These simple compounds play multiple crucial roles in planta and are also important economic traits of oil crops. The enzymatic steps of C18 UFA biosynthesis have been well established. However, the associated FA/lipid trafficking between the plastid and the endoplasmic reticulum remains largely unclear, as does the regulation of the expression and activities of the involved enzymes. In this review, we will revisit the biosynthesis of C18 UFAs with an emphasis on the trafficking, and present an overview of the key enzymes and their regulation. Of particular interest is the emerging regulatory network composed of transcriptional factors and upstream signaling pathways. The review thereby provides the promise of using physical, biochemical and/or genetic means to manipulate FA composition and increase oil yield in crop improvement.

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“…Arabidopsis thaliana mutants and stress assays. All Arabidopsis thaliana mutants used in this study were previously characterized and are all in Col-0 wildtype background: snrk1a2 (WiscDSLox320B03 75,76 ) and snrk1a1 (GABI_579E09 15,75,76 ); snrk3.22 (SALK_118231C 5 ); duf828/fl7 (SALK_077717 60 ) and myb77-2 (SALK_055373C 61 ); afp1-4 afp2-1 45 . Gene accession numbers can be found in Supplementary Data 1.…”

Section: Methodsmentioning

confidence: 99%

An abscisic acid-responsive protein interaction network for sucrose non-fermenting related kinase1 in abiotic stress response

et al. 2020

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Yeast Snf1 (Sucrose non-fermenting1), mammalian AMPK (5′ AMP-activated protein kinase) and plant SnRK1 (Snf1-Related Kinase1) are conserved heterotrimeric kinase complexes that re-establish energy homeostasis following stress. The hormone abscisic acid (ABA) plays a crucial role in plant stress response. Activation of SnRK1 or ABA signaling results in overlapping transcriptional changes, suggesting these stress pathways share common targets. To investigate how SnRK1 and ABA interact during stress response in Arabidopsis thaliana, we screened the SnRK1 complex by yeast two-hybrid against a library of proteins encoded by 258 ABA-regulated genes. Here, we identify 125 SnRK1-interacting proteins (SnIPs). Network analysis indicates that a subset of SnIPs form signaling modules in response to abiotic stress. Functional studies show the involvement of SnRK1 and select SnIPs in abiotic stress responses. This targeted study uncovers the largest set of SnRK1 interactors, which can be used to further characterize SnRK1 role in plant survival under stress.

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SnRK1 phosphorylation of FUSCA3 positively regulates embryogenesis, seed yield, and plant growth at high temperature in Arabidopsis

Abstract: FUS3 phosphorylation at the SnRK1 target site regulates embryo growth rate, seed yield, and plant growth at control and elevated temperature.

Cited by 42 publications

References 55 publications

Root‐specific NF‐Y family transcription factor, PdNF‐YB21, positively regulates root growth and drought resistance by abscisic acid‐mediated indoylacetic acid transport in Populus

Root‐specific NF‐Y family transcription factor, PdNF‐YB21, positively regulates root growth and drought resistance by abscisic acid‐mediated indoylacetic acid transport in Populus

Plant Unsaturated Fatty Acids: Biosynthesis and Regulation

An abscisic acid-responsive protein interaction network for sucrose non-fermenting related kinase1 in abiotic stress response

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