SnRK1 (SNF1‐related kinase 1) has a central role in sugar and ABA signalling in <i>Arabidopsis thaliana</i>

Jossier, Mathieu; Bouly, Jean-Pierre; Meimoun, Patrice; Arjmand, Alaleh; Lessard, Philippe; Hawley, Simon A.; Hardie, D. Grahame; Thomas, Martine

doi:10.1111/j.1365-313x.2009.03871.x

Cited by 291 publications

(272 citation statements)

References 69 publications

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“…Transgenic plants expressing wild-type OsSnRK1 and KIN10 were sensitive to high (6%) Glc (Fig. 4A), which is consistent with the hypersensitivity shown in the presence of high Glc by Arabidopsis seedlings that overexpress KIN10 (Jossier et al, 2009). The effect seemed to be marginal, however, because these transgenic lines did not show a similar developmental arrest phenotype at 4% Glc.…”

Section: Snrk1 Modulates Early Seedling Developmentsupporting

confidence: 67%

“…Arabidopsis SnRK1s (KIN10 and KIN11) have high structural similarity to the catalytic a-subunits of yeast Snf1 and mammalian AMPK (Amodeo et al, 2007;Chen et al, 2009;Xiao et al, 2011) and play similar regulatory roles in stress-inducible gene expression (Baena-González et al, 2007;Jossier et al, 2009;Hardie, 2011). In this study, we used a transient expression system based on Arabidopsis leaf mesophyll protoplasts (Yoo et al, 2007) to demonstrate the evolutionarily conserved gene regulatory functions of rice OsSnRK1.…”

Section: Snrk1 Activates Specific Stress-responsive Gene Expression Imentioning

confidence: 99%

“…To survive such challenges, plant SnRK1s modulate transcriptional, metabolic, and developmental processes (Hey et al, 2010;Smeekens et al, 2010). In Arabidopsis (Arabidopsis thaliana), SnRK1s KIN10 and KIN11 manage energy-depleting conditions through the control of stress-responsive gene expression and stress hormone abscisic acid signaling, which also modulates plant susceptibility to pathogens (Baena-González et al, 2007;Jossier et al, 2009). Rice (Oryza sativa) OsSnRK1, which is under the control of calcineurin B-like interacting protein kinase 15 (Lee et al, 2009), derepresses Glc-repressed gene expression in the embryo to modulate seed germination and early seedling growth (Lu et al, 2007).…”

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confidence: 99%

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Regulatory Functions of SnRK1 in Stress-Responsive Gene Expression and in Plant Growth and Development

et al. 2012

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Sucrose-nonfermentation1-related protein kinase1 (SnRK1) is an evolutionarily conserved energy sensor protein that regulates gene expression in response to energy depletion in plants. Efforts to elucidate the functions and mechanisms of this protein kinase are hampered, however, by inherent growth defects of snrk1-null mutant plants. To overcome these limitations and study SnRK1 functions in vivo, we applied a method combining transient expression in leaf mesophyll protoplasts and stable expression in transgenic plants. We found that both rice (Oryza sativa) and Arabidopsis (Arabidopsis thaliana) SnRK1 activities critically influence stress-inducible gene expression and the induction of stress tolerance. Genetic, molecular, and chromatin immunoprecipitation analyses further revealed that the nuclear SnRK1 modulated target gene transcription in a submergencedependent manner. From early seedling development through late senescence, SnRK1 activities appeared to modulate developmental processes in the plants. Our findings offer insight into the regulatory functions of plant SnRK1 in stressresponsive gene regulation and in plant growth and development throughout the life cycle.

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Section: Snrk1 Modulates Early Seedling Developmentsupporting

confidence: 67%

Section: Snrk1 Activates Specific Stress-responsive Gene Expression Imentioning

confidence: 99%

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confidence: 99%

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Regulatory Functions of SnRK1 in Stress-Responsive Gene Expression and in Plant Growth and Development

et al. 2012

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“…A recent study shows surprisingly that overexpression of the SnRK1.1 protein kinase (Arabidopsis KIN10; Snf1-related PK), which is implicated in the global regulation of transcription and metabolism (Baena-Gonzá lez et al, 2007;Baena-Gonzá lez and Sheen, 2008), causes synergistic hypersensitive response to Glc and ABA (Jossier et al, 2009). Although the expression of more than 1,000 KIN10-regulated genes are negatively correlated with Glc, Suc, and CO 2 levels in plant cells, a supply of Glc can nevertheless activate overexpressed SnRK1.1 and lead to the altered expression of other genes, PATHOGENESIS RELATED1 (PR1), PR2, and PR5, markers of salicylic acid and stress or immune responses (Ramon et al, 2008;Jossier et al, 2009).…”

Section: Connecting the Sugar Signaling Networkmentioning

confidence: 99%

Discover and Connect Cellular Signaling

Sheen

2010

Plant Physiology

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The unique nature of the plant system as a selfsufficient, robust, and resilient organism requires the dynamic coordination of numerous signal transduction pathways in multiple organs and cell types with complementary functions to capture energy and nutrients, to orchestrate growth and development, to adjust or adapt to fluctuating environment, and to live with symbiotic or curb invasive microbes and animals. Plants "move" through their growth and developmental programs highly integrated with the complex environmental cues. Our understanding of plant signal transduction pathways has been greatly facilitated by the isolation and characterization of a wealth of mutant collections in Arabidopsis (Arabidopsis thaliana), rice (Oryza sativa), maize (Zea mays), wheat (Triticum aestivum), barley (Hordeum vulgare), pea (Pisum sativum), tomato (Solanum lycopersicum), Medicago truncatula, moss (Physcomitrella patens), Chlamydomonas reinhardtii, and many other plants. A quantum leap in the field was made possible when the molecular cloning of mutated genes and transgenic plant analysis became a reality in several reference plants in the past two decades. Although the mainstream research on signal transduction has focused on single signals and linear pathways, recent findings have revealed many previously unexpected signaling interconnections, manifesting both the complexity and reality (Fig. 1). The increasing availability of annotated plant genome sequences and large-scale genome-wide databases and computational tools have further empowered the dissection of signaling pathways based on traditional characterization in whole plants, organs, or tissues. Moving forward to discovering and connecting the cellular signaling networks, functional characterization of thousands of genes encoding large families of key regulatory components (Fig. 1) in single cell resolution with time kinetics will be the next challenge. Integrative approaches combing creative genetics, sensitive mass spectrometry, genome-wide screens, powerful bioinformatics tools and skills, versatile cell-based assays, and targeted mutagenesis will be critical for future discoveries (Fig. 2). Glc signaling networks and emerging research tools are briefly highlighted to help pave the way for future research in cellular signaling. CONNECTING THE SUGAR SIGNALING NETWORKCharacterization of plant signaling pathways has been remarkably successful based on single signals (e.g. hormones, stresses, and microbial elicitors) and phenotypic or reporter-based observation of insensitive, constitutive, or hypersensitive response mutants, especially in Arabidopsis and rice. General frameworks, often with linear relationship of positive or negative regulators, for many plant signaling pathways have been established based on the initial signals applied in the mutant screens and molecular identification of the mutated genes. However, Arabidopsis mutant screens with an unconventional signal such as Glc (e.g. at high concentrations to trigger developmental arrest of seedlings) have reve...

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“…As mentioned above, Snf1 in yeast is activated upon Glc depletion and controls many processes through the regulation of transcription factors and metabolic enzymes. SnRK1 in plants represents a master regulator during C and energy deprivation (Baena-González and Sheen, 2008;Halford and Hey, 2009;Jossier et al, 2009). SnRK1.1/KIN10-mediated gene expression is positively correlated with that regulated by C deprivation and negatively correlated with that controlled by sugar (Baena-González et al, 2007).…”

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EXORDIUM-LIKE1 Promotes Growth during Low Carbon Availability in Arabidopsis

2011

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Little is known about genes that control growth and development under low carbon (C) availability. The Arabidopsis (Arabidopsis thaliana) EXORDIUM-LIKE1 (EXL1) gene (At1g35140) was identified as a brassinosteroid-regulated gene in a previous study. We show here that the EXL1 protein is required for adaptation to C-and energy-limiting growth conditions. Indepth analysis of EXL1 transcript levels under various environmental conditions indicated that EXL1 expression is controlled by the C and energy status. Sugar starvation, extended night, and anoxia stress induced EXL1 gene expression. The C status also determined EXL1 protein levels. These results suggested that EXL1 is involved in the C-starvation response. Phenotypic changes of an exl1 loss-of-function mutant became evident only under corresponding experimental conditions. The mutant showed diminished biomass production in a short-day/low-light growth regime, impaired survival during extended night, and impaired survival of anoxia stress. Basic metabolic processes and signaling pathways are presumed to be barely impaired in exl1, because the mutant showed wild-type levels of major sugars, and transcript levels of only a few genes such as QUA-QUINE STARCH were altered. Our data suggest that EXL1 is part of a regulatory pathway that controls growth and development when C and energy supply is poor.

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SnRK1 (SNF1‐related kinase 1) has a central role in sugar and ABA signalling in Arabidopsis thaliana

Cited by 291 publications

References 69 publications

Regulatory Functions of SnRK1 in Stress-Responsive Gene Expression and in Plant Growth and Development

Regulatory Functions of SnRK1 in Stress-Responsive Gene Expression and in Plant Growth and Development

Discover and Connect Cellular Signaling

EXORDIUM-LIKE1 Promotes Growth during Low Carbon Availability in Arabidopsis

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