snRNP: Rich Nuclear Bodies in<i>Hyacinthus orientalis</i>L. Microspores and Developing Pollen Cells

Zienkiewicz, Krzysztof; Bednarska, E

doi:10.1155/2009/209303

Cited by 5 publications

(3 citation statements)

References 43 publications

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“…Initially, we used modified AgNOR staining to visualize nuclear bodies and Cajal bodies in particular (Zienkiewicz & Bednarska ) since REN1 protein co‐localized there as well. Unfortunately, we were not able to observe these structures in Arabidopsis pollen probably due to smaller size of pollen grains and their rougher surface than in Hyacinthus for which this protocol was originally developed.…”

Section: Discussionmentioning

confidence: 99%

“…After maceration, flowers were impregnated by 50% AgNO 3 in water, 55 °C, 20 min; then incubated in 1:1 mixture of 2% gelatin in 1% formic acid and 50% AgNO 3 for 20 min in dark. The plant material was then washed with distilled water for 10 min and then treated with 5% sodium thiosulfate for 10 min (Zienkiewicz & Bednarska ; modified). Individual anthers of sequential developmental series were prepared on glass slide, covered with water, squashed and observed under the microscope (Nicon Eclipse TE2000‐E).…”

Section: Methodsmentioning

confidence: 99%

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A new link between stress response and nucleolar function during pollen development in Arabidopsis mediated by AtREN1 protein

Reňák

Gibalová

Šolcová

et al. 2013

Plant Cell & Environment

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Heat shock transcription factors (Hsfs) are involved in multiple aspects of stress response and plant growth. However, their role during male gametophyte development is largely unknown, although the generative phase is the most sensitive and critical period in the plant life cycle. Based on a wide screen of T-DNA mutant lines, we identified the atren1 mutation (restricted to nucleolus1) in early male gametophytic gene At1g77570, which has the closest homology to HSFA5 gene, the member of a heat shock transcription factor (HSF) gene family. The mutation causes multiple defects in male gametophyte development in both structure and function. Because the mutation disrupts an early acting (AtREN1) gene, these pollen phenotype abnormalities appear from bicellular pollen stage to pollen maturation. Moreover, the consequent progamic phase is compromised as well as documented by pollen germination defects and limited transmission via male gametophyte. In addition, atren1/− plants are defective in heat stress (HS) response and produce notably higher proportion of aberrant pollen grains. AtREN1 protein is targeted specifically to the nucleolus that, together with the increased size of the nucleolus in atren1 pollen, suggests that it is likely to be involved in ribosomal RNA biogenesis or other nucleolar functions.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

A new link between stress response and nucleolar function during pollen development in Arabidopsis mediated by AtREN1 protein

Reňák

Gibalová

Šolcová

et al. 2013

Plant Cell & Environment

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“…Visualization of CBs in living plant cells revealed also that plant CBs exhibit dynamic movement, fusing together and splitting apart within the nucleus (Boudonck et al 1999 ). Moreover, the number of CBs in plant cells was observed to change during the cell cycle and differentiation (Boudonck et al 1998 ; Straatman and Schel 2001 ; Seguí-Simarro et al 2006 ; Zienkiewicz and Bednarska 2009 ). Recently, plant CBs were shown to contain also Atcoilin (Collier et al 2006 ; Koroleva et al 2009 ), which is a homolog of the mammalian protein coilin, a marker of CB in animal cells.…”

Section: Introductionmentioning

confidence: 99%

mRNA accumulation in the Cajal bodies of the diplotene larch microsporocyte

Smoliński

Kolowerzo

2011

Chromosoma

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In microsporocytes of the European larch, we demonstrated the presence of several mRNAs in spherical nuclear bodies. In the nuclei of microsporocytes, we observed up to 12 bodies, ranging from 0.5 to 6 μm in diameter, during the prophase of the first meiotic division. Our previous studies revealed the presence of polyadenylated RNA (poly(A) RNA) in these bodies, but did not confirm the presence of nascent transcripts or splicing factors of the SR family. The lack of these molecules precludes the bodies from being the sites of synthesis and early maturation of primary transcripts (Kołowerzo et al., Protoplasma 236:13–19, 2009). However, the bodies serve as sites for the accumulation of splicing machinery, including the Sm proteins and small nuclear RNAs. Characteristic ultrastructures and the molecular composition of the nuclear bodies, which contain poly(A) RNA, are indicative of Cajal bodies (CBs). Here, we demonstrated the presence of several housekeeping gene transcripts—α-tubulin, pectin methylesterase, peroxidase and catalase, ATPase, and inositol-3-phosphate synthase—in CBs. Additionally, we observed transcripts of the RNA polymerase II subunits RPB2 and RPB10 RNA pol II and the core spliceosome proteins mRNA SmD1, SmD2, and SmE. The co-localization of nascent transcripts and mRNAs indicates that mRNA accumulation/storage, particularly in CBs, occurs in the nucleus of microsporocytes.Electronic supplementary materialThe online version of this article (doi:10.1007/s00412-011-0339-4) contains supplementary material, which is available to authorized users.

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Periodic expression of Sm proteins parallels formation of nuclear Cajal bodies and cytoplasmic snRNP-rich bodies

Smoliński

Wróbel

Noble

et al. 2011

Histochem Cell Biol

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Small nuclear ribonucleoproteins (snRNPs) play a fundamental role in pre-mRNA processing in the nucleus. The biogenesis of snRNPs involves a sequence of events that occurs in both the nucleus and cytoplasm. Despite the wealth of biochemical information about the cytoplasmic assembly of snRNPs, little is known about the spatial organization of snRNPs in the cytoplasm. In the cytoplasm of larch microsporocytes, a cyclic appearance of bodies containing small nuclear RNA (snRNA) and Sm proteins was observed during anther meiosis. We observed a correlation between the occurrence of cytoplasmic snRNP bodies, the levels of Sm proteins, and the dynamic formation of Cajal bodies. Larch microsporocytes were used for these studies. This model is characterized by natural fluctuations in the level of RNA metabolism, in which periods of high transcriptional activity are separated from periods of low transcriptional activity. In designing experiments, the authors considered the differences between the nuclear and cytoplasmic phases of snRNP maturation and generated a hypothesis about the direct participation of Sm proteins in a molecular switch triggering the formation of Cajal bodies.Electronic supplementary materialThe online version of this article (doi:10.1007/s00418-011-0861-8) contains supplementary material, which is available to authorized users.

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snRNP: Rich Nuclear Bodies inHyacinthus orientalisL. Microspores and Developing Pollen Cells

Cited by 5 publications

References 43 publications

A new link between stress response and nucleolar function during pollen development in Arabidopsis mediated by AtREN1 protein

A new link between stress response and nucleolar function during pollen development in Arabidopsis mediated by AtREN1 protein

mRNA accumulation in the Cajal bodies of the diplotene larch microsporocyte

Periodic expression of Sm proteins parallels formation of nuclear Cajal bodies and cytoplasmic snRNP-rich bodies

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