So many rhizobial partners, so little nitrogen fixed: The intriguing symbiotic promiscuity of common bean (Phaseolus vulgaris L.)

Moura, Fernanda Terezinha; Ribeiro, Renan Augusto; Helene, Luisa Caroline Ferraz; Nogueira, Marco Antônio; Hungría, Mariangela

doi:10.1007/s13199-022-00831-6

Cited by 14 publications

(5 citation statements)

References 84 publications

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“…In addition, contrarily to the observations on soybean, no differences were observed, in the UniFrac analysis, in common bean nodules as an effect of either inoculation or co-inoculation. The higher relative abundance of other genera in common bean nodules also confirms the high promiscuity of the legume, detected in studies using classical isolation procedures, e.g., [ 14 , 18 , 44 , 45 ], and now confirmed by sequencing of the whole nodule microbiome. The common bean microbiome also included several other alpha- and beta-rhizobia, in agreement with reports of isolation of these genera using culture-based methods, e.g., [ 46 , 47 ].…”

Section: Discussionsupporting

confidence: 74%

Microbiome of Nodules and Roots of Soybean and Common Bean: Searching for Differences Associated with Contrasting Performances in Symbiotic Nitrogen Fixation

Bender

Alves²,

Silva³

et al. 2022

IJMS

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Biological nitrogen fixation (BNF) is a key process for the N input in agriculture, with outstanding economic and environmental benefits from the replacement of chemical fertilizers. However, not all symbioses are equally effective in fixing N2, and a major example relies on the high contribution associated with the soybean (Glycine max), contrasting with the low rates reported with the common bean (Phaseolus vulgaris) crop worldwide. Understanding these differences represents a major challenge that can help to design strategies to increase the contribution of BNF, and next-generation sequencing (NGS) analyses of the nodule and root microbiomes may bring new insights to explain differential symbiotic performances. In this study, three treatments evaluated in non-sterile soil conditions were investigated in both legumes: (i) non-inoculated control; (ii) inoculated with host-compatible rhizobia; and (iii) co-inoculated with host-compatible rhizobia and Azospirillum brasilense. In the more efficient and specific symbiosis with soybean, Bradyrhizobium presented a high abundance in nodules, with further increases with inoculation. Contrarily, the abundance of the main Rhizobium symbiont was lower in common bean nodules and did not increase with inoculation, which may explain the often-reported lack of response of this legume to inoculation with elite strains. Co-inoculation with Azospirillum decreased the abundance of the host-compatible rhizobia in nodules, probably because of competitiveness among the species at the rhizosphere, but increased in root microbiomes. The results showed that several other bacteria compose the nodule microbiomes of both legumes, including nitrogen-fixing, growth-promoters, and biocontrol agents, whose contribution to plant growth deserves further investigation. Several genera of bacteria were detected in root microbiomes, and this microbial community might contribute to plant growth through a variety of microbial processes. However, massive inoculation with elite strains should be better investigated, as it may affect the root microbiome, verified by both relative abundance and diversity indices, that might impact the contribution of microbial processes to plant growth.

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Section: Discussionsupporting

confidence: 74%

Microbiome of Nodules and Roots of Soybean and Common Bean: Searching for Differences Associated with Contrasting Performances in Symbiotic Nitrogen Fixation

Bender

Alves²,

Silva³

et al. 2022

IJMS

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“…All tested bacteria were also able to form nodules on common bean (Table S2). This plant is known for establishing promiscuous symbioses, since it can associate with a broad variety of rhizobia species (Moura et al 2022). Indeed, previous studies have indicated the possibility of nodule formation by Paraburkholderia spp.…”

Section: Discussionmentioning

confidence: 99%

“…Common bean (Phaseolus vulgaris L.) was also used as an additional host since it is a promiscuous papilionoid legume (Moura et al 2022). Fourteen bacterial isolates were selected for this experiment according to their origin (host and site).…”

Section: Bacteria Isolation and Plant Testsmentioning

confidence: 99%

Paraburkholderia atlantica is the main rhizobial symbiont of Mimosa spp. in ultramafic soils in the Brazilian Cerrado biome

Neto

Ribeiro²,

Fernandes‐Júnior³

et al. 2022

Plant Soil

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were characterized for their nodulation capacity on M. pudica and common bean, and their tolerance to Ni in culture medium. Bacteria were also partially identified by their 16S rRNA gene sequences. In addition, recA, gyrB, nodC and nifH genes from five representative isolates were sequenced for phylogenetic studies. Results In situ detection indicated the exclusive presence of Paraburkholderia sp. within the nodules. This identification was confirmed for most of the isolates by the analysis of their 16S rRNA gene sequences. All isolates identified as Paraburkholderia sp. were able to effectively nodulate M. pudica, but those tested in common bean produced ineffective

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“…Among these are Ensifer spp., Pararhizobium spp., Bradyrhizobium spp., Cupriavidus spp., and Paraburkholderia spp. [ 42 , 45 , 46 ]. However, the interaction with these species occurs at different effectiveness levels.…”

Section: Microsymbionts Inhabitants Of Pulse Nodulesmentioning

confidence: 99%

Contribution of Biofertilizers to Pulse Crops: From Single-Strain Inoculants to New Technologies Based on Microbiomes Strategies

Xavier

Jesus

Dias

et al. 2023

Plants

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Pulses provide distinct health benefits due to their low fat content and high protein and fiber contents. Their grain production reaches approximately 93,210 × 103 tons per year. Pulses benefit from the symbiosis with atmospheric N2-fixing bacteria, which increases productivity and reduces the need for N fertilizers, thus contributing to mitigation of environmental impact mitigation. Additionally, the root region harbors a rich microbial community with multiple traits related to plant growth promotion, such as nutrient increase and tolerance enhancement to abiotic or biotic stresses. We reviewed the eight most common pulses accounting for almost 90% of world production: common beans, chickpeas, peas, cowpeas, mung beans, lentils, broad beans, and pigeon peas. We focused on updated information considering both single-rhizobial inoculation and co-inoculation with plant growth-promoting rhizobacteria. We found approximately 80 microbial taxa with PGPR traits, mainly Bacillus sp., B. subtilis, Pseudomonas sp., P. fluorescens, and arbuscular mycorrhizal fungi, and that contributed to improve plant growth and yield under different conditions. In addition, new data on root, nodule, rhizosphere, and seed microbiomes point to strategies that can be used to design new generations of biofertilizers, highlighting the importance of microorganisms for productive pulse systems.

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So many rhizobial partners, so little nitrogen fixed: The intriguing symbiotic promiscuity of common bean (Phaseolus vulgaris L.)

Cited by 14 publications

References 84 publications

Microbiome of Nodules and Roots of Soybean and Common Bean: Searching for Differences Associated with Contrasting Performances in Symbiotic Nitrogen Fixation

Microbiome of Nodules and Roots of Soybean and Common Bean: Searching for Differences Associated with Contrasting Performances in Symbiotic Nitrogen Fixation

Paraburkholderia atlantica is the main rhizobial symbiont of Mimosa spp. in ultramafic soils in the Brazilian Cerrado biome

Contribution of Biofertilizers to Pulse Crops: From Single-Strain Inoculants to New Technologies Based on Microbiomes Strategies

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