“…Artemia nauplii remain the most essential food for marine crustacean larvae and fish due to their nutritional quality, digestibility, ability to combine the benefits of live and dry diets, and convenience of dry cysts that can hatch within 24 h (Bardócz et al, 1999;Nkambo et al, 2019;Van Stappen et al, 2020). Although Artemia nauplii have been widely used in other freshwater prawn larval culture (Kovalenko et al, 2002;Barros and Valenti, 2003;De Aviz et al, 2018), their use in larval rearing of M. rude larvae has not been documented. In addition, optimal feeding density of Artemia nauplii is essential for the survival of M. rude and avoid overfeeding, which can lead to poor water quality, high costs of tank cleaning and maintenance, and stress on larvae (De Aviz et al, 2018).…”