Social and Behavioural Factors Associated with Dental Caries Experience among Adolescent School Children in Bengaluru City, India

et al. 2021

JPRI

Background: Stevia rebaudiana is a shrub-like plant that belongs to the sunflower family and is commonly referred as stevia.It is 1000 times sweeter than sugar even at a very low concentration. Xanthine oxidase is an enzyme that generates oxygen species and catalyzes the production of uric acid from purine metabolism.Overproduction of uric acid results in a clinical condition called gout. The aim of this study is to explore the phytochemicals, antioxidant and xanthine oxidase inhibitory potential of methanolic leaf extract of stevia rebaudiana. Methods: Methanolic leaf extract of Stevia rebaudiana was prepared by the Hot Percolation method. Phytochemical screening was done to analyse the presence of various phytochemicals. The leaf extract was tested for its antioxidant and xanthine oxidase inhibitory potentials. The data were analyzed statistically by a one-way analysis of variance (ANOVA) followed by Duncan’s multiple range test was used to see the statistical significance among the groups. The results with the p<0.05 level were considered to be statistically significant. Results: It was observed that the methanolic leaf extract of Stevia rebaudiana has significant antioxidant potential (Ic50 of = 310 μg/ml) as well as xanthine oxidase inhibitory potentials(Ic50 of = 270 μg/ml) and the activity increased in a dose dependent manner as compared to that of standard (Vitamin C and Allopurinol respectively). Conclusion: The study proves the antioxidant and xanthine oxidase inhibitory efficacy of Stevia rebaudiana and throws light on the prospects of drug formation against oxidant activity and gout formation.

Section: Introductionmentioning

confidence: 99%

Evaluation of Antioxidant and Xanthine Oxidase Inhibitory Potential of Methanolic Leaf Extract of Stevia rebaudiana

Rishikesan

et al. 2021

JPRI

“…Over the years, one of the most popular methods to study in vitro antiinflammatory properties of plant extract is done by Albumin denaturation inhibition assay and antioxidant potential by DPPH assay [20][21]. Our team has extensive knowledge and research experience that has translate into high quality publications [22][23][24][25][26][27][28][29][30][31][32][33][34][35][36]. The main aim of this study is to assess the phytoconstituents, antioxidant and in vitro anti denaturation potential of methanolic root extract of Acorus calamus.…”

Section: Introductionmentioning

confidence: 99%

Phytochemical Screening and In vitro Albumin Denaturation Inhibitory Potential of Methanolic Root Extract of Acorus calamus

Varshan

et al. 2021

JPRI

Introduction: Medicinal plants are chief antidotes for numerous diseases and have been used since time immemorial. Sweet flag’s (Acorus calamus) presence is in Ayurveda and belongs to the genus Acorus L. of the family Acoraceae and is widely distributed in temperate to sub temperate regions. It is commonly used to treat appetite loss, diarrhoea, digestive disorders in traditional medicinal systems of Asian and European countries. The aim of this study is to explore the phytoconstituents, antioxidant and anti-inflammatory potential of methanolic root extract of Acorus calamus. Materials and Methods: Methanolic root extract of Acorus calamus was done by the Hot Percolation method. Later it was dried and used to analyse the antioxidant and anti-inflammatory potentials. Phytochemical screening was done to analyse the presence of various phytochemicals. Antioxidant effect of Acorus calamus was tested by 2, 2-Diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity and Albumin denaturation inhibitory potential test was organised for testing it’s anti-inflammatory Activity. The data were analysed statistically by a one-way analysis of Variance (ANOVA) followed by Duncan’s multiple range test was used to see the statistical significance among the groups. The results with the p<0.05 level were considered to be statistically significant. Results: Methanolic root extract of Acorus calamus was found to be rich in Alkaloids, Flavonoids, Terpenoids, Sapanoids, Steroids and Phlobatannin. The presence of phytochemicals like alkaloids, saponins, Flavonoids indicates that the extract has potential for further in vitro analysis like antioxidant and anti-inflammatory potentials. It was observed that Acorus calamus has both antioxidant (IC50 of = 295 µg/ml) as well as anti inflammatory potentials (IC50 =310 µg/ml) and the activity increased in a dose dependent manner as compared to that of standard (Vitamin C and Diclofenac respectively). Conclusion: The study proves the antioxidant and anti-inflammatory efficacy of Acorus calamus and throws light on the prospects of drug formulation against oxidant activity and inflammation.

“…Our department has substantial knowledge and research encounters that have led to immense quality publications. [12][13][14][15][16][17][18][19][20][21][22][23][24][25][26][27][28][29][30][31]. This research aimed to evaluate the anti cholesterol and antioxidant potential of aqueous extract of Citrus aurantifolia, Zingiber offcinale and its formulation through in vitro analysis.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Anti Cholesterol and Antioxidant Potentiality of Aqueous Extracts of Citrus aurantifolia, Zingiber officinale and its Formulation - a Comparative In vitro Study

Sivasubramanian

et al. 2021

JPRI

Background: Several plant extracts and herbs have been used for treating and prevention of cardiovascular diseases hypertension, angina pectoris, congestive heart failure, atherosclerosis, cerebral and venous insufficiency and arrhythmia. Similarly, aqueous extracts of Citrus aurantifolia and Zingiber officinale which is commonly called as lemon and ginger respectively and its formulation were analysed for its antioxidant and anti cholesterol activity. Objective: This research has been performed in order to evaluate the anti cholesterol and antioxidant potentiality of aqueous extracts of Citrus aurantifolia, Zingiber officinale and its formulation respectively. Methods: The study setting carried out for this research was in vitro, hence the work was performed outside the living organism. Citrus aurantifolia and Zingiber officinale were purchased from a farm in chennai. The experiment began starting from the preparation of aqueous extract of lemon and ginger. A formulation was made combining equal amounts of the two extracts followed by this, a phytochemical screening test was conducted. Antioxidant and anti cholesterol potential of the extracts and its formulation were also analysed. The data was statistically examined by one-way analysis of variance (ANOVA) continued by Duncan’s multiple range test, it was compiled to see the statistical significance among the groups present. The results carrying p<0.05 level were contemplated to be statistically significant. Result: From the study, though both the extracts possessed a good antioxidant and anti cholesterol potential, comparatively the formulation exhibited an increased antioxidant and anti cholesterol potential respectively (IC₅₀ = 250 µg/ml) and (IC₅₀= 375µg per ml). Here, the formulation exhibited significantly more activity than the individual extracts. Conclusion: Even though there is sufficient knowledge among citizens about the nutritional value present in herbal formulations, there isn’t enough in-depth study conducted on the formulation of these two extracts based on their cholesterol inhibitory activity. From this particular study it was proven that the formulation showed synergism. Hence the formulation of these extracts could be preferred over other synthetic drugs since it is natural, cost effective and easily accessible.