Rural regeneration is a transformative process that can be based on multiple possible pathways that are not always clearly distinct and that can be combined or co-exist. We argue that regeneration can be understood as a new framework for socio-natural relations. This paper aims at demonstrating that the combination of agroecology and strong multifunctional agriculture play a central role in re-producing the conditions of socio-ecological wellbeing. The study uses data collected through a qualitative methodology. Two case studies were executed in two Southern Italian regions: the first on farm successors in Sicily and the second on new entrants to farming in Apulia. In total, 41 in depth-interviews, two focus groups, and two restitution meetings were conducted. Findings suggest that agroecology and strong multifunctionality are innovative approaches to farming, allowing new farmers to deal with social, environmental, and economic production challenges, spreading benefits to the rural areas where they operate. The conclusion highlights that policies need to be more effective to support these processes.