Social isolation in rats inhibits oxidative metabolism, decreases the content of mitochondrial K-Ras and activates mitochondrial hexokinase

Zhuravliova, Elene; Barbakadze, Tamar; Zaalishvili, Elnari; Chipashvili, M. D.; Koshoridze, Nana; Mikeladze, David

doi:10.1016/j.bbr.2009.07.009

Cited by 31 publications

(28 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The studied enzymes included aconitase, an enzyme from the citrate cycle. As previous studies suggest [21,24], the active center of aconitase is represented by an iron-sulfurous cluster, which is the reason why it shows high sensitivity toward active forms of oxygen. It can be possibly used as a specific marker, sensitive to the oxidative stress.…”

Section: Activity Of Energy Metabolism Enzymes Under Stressmentioning

confidence: 99%

See 1 more Smart Citation

Functional state of rat cardiomyocytes and blood antioxidant system under psycho-emotional stress

Kuchukashvili¹,

Menabde²,

Chachua³

et al. 2011

ABBS

Self Cite

View full text Add to dashboard Cite

We studied the functionality of the antioxidant system in laboratory rat cardiomyocytes and blood under psychoemotional stress. It was found that 40-day isolation and violation of diurnal cycle among the animals were accompanied by the intensification of lipid peroxidation process and marked with a reduced activity of antioxidant system enzymes, such as catalase and superoxide dismutase activity. The results suggested that psycho-emotional stress was accompanied by oxidative stress, causing a reduction in the intensity of energy metabolism in cardiomyocytes, which was further strengthened by the fact that the activity of the enzymes involved in ATP synthesis in mitochondria was reduced. Based on the results, we proposed that psychological stress is one of the factors contributing to the development of various cardiac diseases.

show abstract

Section: Activity Of Energy Metabolism Enzymes Under Stressmentioning

confidence: 99%

“…) [21]. Activity of creatine kinase was determined by NADH formation following absorbance at 340 nm at 258C.…”

mentioning

confidence: 99%

Functional state of rat cardiomyocytes and blood antioxidant system under psycho-emotional stress

Kuchukashvili¹,

Menabde²,

Chachua³

et al. 2011

ABBS

Self Cite

View full text Add to dashboard Cite

show abstract

“…In the meanwhile, these mice showed behavioral changes relevant to some symptoms seen in schizophrenia 97,98 and (5) Ketamine was shown to induce mitochondrial dysfunction, while produced behavioral changes 99 . Moreover, social isolation rearing inhibited oxidative metabolism and induced oxidative stress in rats 100,101 , while it produced several behavioral outcomes similar to those observed in humans with early life stress [100][101][102] . Taken together, these previous studies suggest a connection between mitochondrial dysfunction and neuroinflammation in schizophrenia.…”

Section: Neuroinflammation and Apoptosismentioning

confidence: 52%

Neuroinflammation in Schizophrenia Focused on the Pharmacological and Therapeutic Evidence

Xu¹

2015

Pharmacologia

View full text Add to dashboard Cite

Background: Schizophrenia is a heterogeneous mental disorder with a variety of symptoms. Although, there are no pathognomonic abnormalities in brains of schizophrenia patients, recent years have witnessed research progresses in revealing pathologic changes in cellular and molecular levels. Objective: This article reviewed recent human studies showing neuroimmune alterations in schizophrenia patients and offered explanations for roles of neuroinflammation in the pathogenesis of schizophrenia by citing some of experimental data from non-human studies. The focus of this review was put on pharmacological and therapeutic evidence pointing to a recommendation of anti-inflammatory treatment for patients with schizophrenia. Particularly, it provided compelling evidence supporting an anti-inflammatory effect of antipsychotics by reviewing a relatively large body of studies in the categories of in vitro, animals and humans. Conclusion: Then, it reviewed recent clinical trials with minocycline, a second-generation tetracycline, or the selective COX-2 inhibitor celecoxib. Most of these clinical trials provided promising results of superior beneficial treatment effects as the consequence of co-administration of standard antipsychotic drugs and anti-inflammatory compounds, compared with antipsychotic drugs alone.

show abstract

“…Mitochondrion is one of the main targets of Ca-dependent Ras-trafficking; however, the functions of K-Ras in mitochondria are not fully understood (Bivona et al 2006;Philips 2006). Our previous observation suggests that the content of mitochondrial K-Ras in hippocampus is changed in various stress conditions (Zhuravliova et al 2009) and delocalization of K-Ras is accompanied by the inhibition of mitochondrial oxidative metabolism. Everything together suggests that the translocation of K-Ras in synaptic mitochondria is a specific response to the energy metabolism impairment in the synaptic endings.…”

Section: Discussionmentioning

confidence: 95%

Synaptic and Non-Synaptic Mitochondria in Hippocampus of Adult Rats Differ in Their Sensitivity to Hypothyroidism

et al. 2012

View full text Add to dashboard Cite

Hypothyroidism in humans provokes various neuropsychiatric disorders, movement, and cognitive abnormalities that may greatly depend on the mitochondrial energy metabolism. Brain cells contain at least two major populations of mitochondria that include the non-synaptic mitochondria, which originate from neuronal and glial cell bodies (CM), and the synaptic (SM) mitochondria, which primarily originate from the nerve terminals. Several parameters of oxidative stress and other parameters in SM and CM fractions of hippocampus of adult rats were compared among euthyroid (control), hypothyroid (methimazol-treated), and thyroxine (T4)-treated hypothyroid states. nNOS translocation to CM was observed with concomitant increase of mtNOS's activity in hypothyroid rats. In parallel, oxidation of cytochrome c oxidase and production of peroxides with substrates of complex I (glutamate + malate) were enhanced in CM, whereas the activity of aconitase and mitochondrial membrane potential (ΔΨm) were decreased. Furthermore, the elevation of mitochondrial hexokinase activity in CM was also found. No differences in these parameters between control and hypothyroid animals were observed in SM. However, in contrast to CM, hypothyroidism increases the level of pro-apoptotic K-Ras and Bad in SM. Our results suggest that hypothyroidism induces moderate and reversible oxidative/nitrosative stress in hippocampal CM, leading to the compensatory elevation of hexokinase activity and aerobic glycolysis. Such adaptive activation in glycolytic metabolism does not occur in SM, suggesting that synaptic mitochondria differ in their sensitivity to the energetic disturbance in hypothyroid conditions.

show abstract

Social isolation in rats inhibits oxidative metabolism, decreases the content of mitochondrial K-Ras and activates mitochondrial hexokinase

Cited by 31 publications

References 47 publications

Functional state of rat cardiomyocytes and blood antioxidant system under psycho-emotional stress

Functional state of rat cardiomyocytes and blood antioxidant system under psycho-emotional stress

Neuroinflammation in Schizophrenia Focused on the Pharmacological and Therapeutic Evidence

Synaptic and Non-Synaptic Mitochondria in Hippocampus of Adult Rats Differ in Their Sensitivity to Hypothyroidism

Contact Info

Product

Resources

About