<i>Background</i>: Non-communicable diseases (NCDs) are the leading causes of poor health and early death globally. Although NCDs are most common in middle to late adulthood, many lifestyle habits that contribute to these diseases begin during adolescence. This research evaluated the knowledge of non-communicable diseases among adolescents in public and private secondary schools in Port Harcourt, Rivers State. <i>Methods</i>: The study used a comparative cross-sectional design to collect research data from 640 adolescents. Participants were selected through a multi-stage sampling technique and data was analysed using IBM Statistical Product for the Service Solution version 29. <i>Results</i>: Few respondents from private (6.3%) and public (5.3%) schools had good knowledge, majority from private (75%) and public (66.9) schools had fair knowledge, while 18.8% (private) and 27.8% (public) had poor knowledge on non-communicable diseases. Socioeconomic status and knowledge level were statistically significant (p<0.05). The prevalence of NCDs (asthma, diabetes, high blood pressure) was 3.4% and 2.5% among private and public school adolescents. <i>Conclusion</i>: There is need for continuous health education on NCDs risk factors among adolescents to ensure they adopt healthy lifestyles through informed choices to foster good and quality health into adulthood.