Socket augmentation using a commercial collagen-based product — an animal study in pigs

Kunert‐Keil, Christiane; Gredes, Tomasz; Heinemann, Friedhelm; Dominiak, Marzena; Botzenhart, Ute; Gedrange, Tomasz

doi:10.1016/j.msec.2014.10.033

Cited by 22 publications

(24 citation statements)

References 22 publications

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“…while the porosity of the membrane allows for the ingrowth of bone-forming cells [22]. Thus, the membrane can play a role in stabilizing the blood clot, maintaining space for bone regeneration in the socket, and protecting the wound from mechanical disruption and saliva contamination.…”

Section: Biomed Research Internationalmentioning

confidence: 99%

Ridge Alterations following Socket Preservation Using a Collagen Membrane in Dogs

Lyu

Shao

Zou

et al. 2020

BioMed Research International

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Background. The healing process following tooth extraction results in alveolar ridge resorption. The dimensional changes may complicate the subsequent implant procedure. Socket preservation using absorbable collagen membranes or a combination of membranes with calcium phosphate cement (CPC) particles might ensure that the alveolar ridge retains a suitable morphology for implant placement. Objective. To evaluate the quality and quantity of new bone regenerated after application of either collagen membranes alone covering the sockets or a combination of membranes with CPC particles added into the sockets in dogs. Materials and Methods. Six dogs were included in this study. The mandibular premolars were extracted. For each hemimandible, three premolar extraction sites were randomly assigned to one of the following treatments: a covering collagen membrane, CPC with a covering collagen membrane, and a socket left empty. Cone-beam computed tomography (CBCT) measurements, polyfluorochrome sequential labeling, and histological assessments were performed to investigate the healing ability and repair processes within a 6-month observation period. Results. Buccal bone height in the membrane group was significantly higher than that in the membrane+CPC and blank groups at 4 and 6 months after extraction. The mineral apposition rate over 2-4 months and the alizarin red-stained area in the membrane group were significantly higher than those in the other two groups. Histological analysis after 6 months of healing showed significantly higher amounts of newly formed bone in the membrane group than in the other groups. Conclusion. Extraction sites treated with collagen barrier membranes showed better protection than sites not covered with membranes. And the buccal bone wall of the socket was well preserved by collagen membrane without extra CPC materials. Socket preservation using absorbable membranes alone yielded better quality and quantity of regenerated bone inside the socket site.

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Section: Biomed Research Internationalmentioning

confidence: 99%

Ridge Alterations following Socket Preservation Using a Collagen Membrane in Dogs

Lyu

Shao

Zou

et al. 2020

BioMed Research International

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“…This is also true for transient receptor potential melastatin 3 (TRPM3; the nomenclature in this paper follows BJP's Concise Guide to PHARMACOLOGY, Alexander et al, 2013), one of the least studied members of this important class of ion channels (Oberwinkler and Philipp, 2007). Transcripts encoding for TRPM3 channels have been described in a variety of tissues and cell types (Grimm et al, 2003;Lee et al, 2003;Oberwinkler et al, 2005;Fonfria et al, 2006;Kunert-Keil et al, 2006;Wagner et al, 2008;Vriens et al, 2011). However, in most of the TRPM3-expressing tissues, the function of these channels is not well understood.…”

Section: Introductionmentioning

confidence: 99%

Structural requirements of steroidal agonists of transient receptor potential melastatin 3 (TRPM3) cation channels

Drews

Mohr

Rizun

et al. 2014

British J Pharmacology

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Background and PurposeTransient receptor potential melastatin 3 (TRPM3) proteins form non-selective but calcium-permeable membrane channels, rapidly activated by extracellular application of the steroid pregnenolone sulphate and the dihydropyridine nifedipine. Our aim was to characterize the steroid binding site by analysing the structural chemical requirements for TRPM3 activation.Experimental ApproachWhole-cell patch-clamp recordings and measurements of intracellular calcium concentrations were performed on HEK293 cells transfected with TRPM3 (or untransfected controls) during superfusion with pharmacological substances.Key ResultsPregnenolone sulphate and nifedipine activated TRPM3 channels supra-additively over a wide concentration range. Other dihydropyridines inhibited TRPM3 channels. The natural enantiomer of pregnenolone sulphate was more efficient in activating TRPM3 channels than its synthetic mirror image. However, both enantiomers exerted very similar inhibitory effects on proton-activated outwardly rectifying anion channels. Epiallopregnanolone sulphate activated TRPM3 almost equally as well as pregnenolone sulphate. Exchanging the sulphate for other chemical moieties showed that a negative charge at this position is required for activating TRPM3 channels.Conclusions and ImplicationsOur data demonstrate that nifedipine and pregnenolone sulphate act at different binding sites when activating TRPM3. The latter activates TRPM3 by binding to a chiral and thus proteinaceous binding site, as inferred from the differential effects of the enantiomers. The double bond between position C5 and C6 of pregnenolone sulphate is not strictly necessary for the activation of TRPM3 channels, but a negative charge at position C3 of the steroid is highly important. These results provide a solid basis for understanding mechanistically the rapid chemical activation of TRPM3 channels.

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“…For quantification of the protein expression levels, 5–10 digital pictures with a magnification of x200 were randomly taken in different areas of each section (Zeiss light microscope, Axio Scope A1 equipped with a digital camera AxioCam MRc, Zeiss, Jena, Germany). For all sections, the quantity of pixels that had a positive reaction for cav-1 and CD45 was assessed using cell^F Ink software (analySIS Image Processing Olympus, Münster) as described by Kunert-Keil et al, [40]. …”

Section: Methodsmentioning

confidence: 99%

In vivo analysis of covering materials composed of biodegradable polymers enriched with flax fibers

et al. 2017

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BackgroundThe objective of this study was to investigate the in vivo effect of bioactive composites with poly(lactic acid) (PLA) or polycaprolactone (PCL) as the matrix, reinforced with bioplastic flax fibers, on the surrounding muscle tissue.MethodsMaterials of pure PLA and PCL and their composites with flax fibers from genetically modified plants producing poly-3-hydroxybutyrate (PLA-transgen, PCL-transgen) and unmodified plants (PLA-wt, PCL-wt) were placed subcutaneous on the M. latissimus dorsi for four weeks.ResultsThe analysis of histological samples revealed that every tested material was differently encapsulated and the capsule thickness is much more pronounced when using the PCL composites in comparison with the PLA composites. The encapsulation by connective tissue was significantly reduced around PCL-transgen and significantly increased in the cases of PLA-transgen and PLA-wt. In the collected muscle samples, the measured protein expression of CD45, lymphocyte common antigen, was significantly increased after the use of all tested materials, with the exception of pure PCL. In contrast, the protein expression of caveolin-1 remained unchanged after treatment with the most examined materials. Only after insertion of PLA-wt, a significant increase of caveolin-1 protein expression was detected, due to the improved neovascularization.ConclusionThese data support the presumption that the new bioactive composites are biocompatible and they could be applicable in the medical field to support the regenerative processes.

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Socket augmentation using a commercial collagen-based product — an animal study in pigs

Cited by 22 publications

References 22 publications

Ridge Alterations following Socket Preservation Using a Collagen Membrane in Dogs

Ridge Alterations following Socket Preservation Using a Collagen Membrane in Dogs

Structural requirements of steroidal agonists of transient receptor potential melastatin 3 (TRPM3) cation channels

In vivo analysis of covering materials composed of biodegradable polymers enriched with flax fibers

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