SOD and catalase inactivation by singlet oxygen and peroxyl radicals

Escobar, Jorge; Rubio, Marı́a A.; Lissi, E. A.

doi:10.1016/0891-5849(95)02037-3

Cited by 305 publications

(150 citation statements)

References 17 publications

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“…Increased SOD activity could be regarded as an indication that the antioxidant mechanism of the brain is activated in response to oxidative stress (26). Free radicals directly damage antioxidant enzymes and reduce their activities (27). In the present study, GSH content was found to be significantly decreased in VD rats.…”

Section: Discussionsupporting

confidence: 50%

Protective effect of bilobalide on learning and memory impairment in rats with vascular dementia

Huang

et al. 2013

Molecular Medicine Reports

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Abstract. Vascular dementia (VD) is the second most common type of dementia in the elderly. Currently, there are no effective drugs for preventing or decreasing the progression of dementia. Bilobalide (BB) is a monomer extracted from Ginkgo biloba leaves. The present study investigated the neuroprotective effects of BB in a two-vessel occlusion (2-VO)-induced VD rat model. The results showed that BB (4 and 8 mg/kg) significantly protected VD rats against cognitive deficits in the Morris water maze. Biochemical assessment showed that BB (4 and 8 mg/kg) increased superoxide dismutase (SOD) activity and glutathione (GSH) content, and decreased nitric oxide synthase (NOS) activity and malondialdehyde (MDA) content. Additionally, BB (4 and 8 mg/kg) was found to alleviate neuronal apoptosis and to reduce the expression of tumor necrosis factor-α (TNF-α) in the brain cortex and the hippocampal CA1 region in VD rats. These results suggest that BB provides protection against learning and memory impairment by reducing free radical injury and inhibiting neuronal apoptosis in the brain cortex and hippocampal CA1 region in VD rats.

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Section: Discussionsupporting

confidence: 50%

Protective effect of bilobalide on learning and memory impairment in rats with vascular dementia

Huang

et al. 2013

Molecular Medicine Reports

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“…Fish from the polluted site had a lower gill CAT activity, while the gill SOD activity was similar in fish from both sites. It was reported that singlet oxygen, superoxide and peroxyl radicals are CAT inhibitors [28] . These two facts should imply an exacerbation of tissue oxidative stress, since H 2 O 2 production as a consequence of SOD activity remains stable but the capacity of H 2 O 2 degradation is lower in fish from the polluted site due to its lower CAT activity.…”

Section: Discussionmentioning

confidence: 99%

Hepatic Histopathological Characteristics and Antioxidant Response of Phytoplanktivorous Silver Carp Intraperitoneally Injected with Extracted Microcystins

Li¹,

Xie²

2009

Biomedical and Environmental Sciences

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“…Apoptosis induction under all conditions was blocked by the NADPH oxidase (NOX) inhibitor 4-(2-aminoethyl)benzenesulfonyl fluoride (AEBSF) and the hydroxyl radical scavenger mannitol, in line with the established central role of superoxide anions and hydroxyl radicals in both signaling pathways (Heinzelmann and Bauer, 2010;Bauer, 2012). Extracellular catalase activity with modulatory potential, at a concentration that was too low for complete protection against intercellular apoptosis-inducing ROS signaling, was further confirmed for 208Fsrc3 using additional approaches, such as inactivation of catalase by extracellular singlet oxygen (Escobar et al, 1996) generated by the photosensitizer photofrin (data not shown) or by the reactive aldehyde 4-hydroxynonenal (D'Souza et al, 2008;Bauer and Zarkovic, 2015). Extracellular catalase was demonstrated after induction of RAS expression in IR-1 cells (208F rat fibroblasts with an inducible RAS oncogene) (Schwieger et al, 2001), accompanied by NOX1 activation and transformation and in spontaneously transformed hamster embryonal (STHE) fibroblasts (Deichman et al, 1989) (data not shown).…”

Section: Extracellular Catalase Modulates Intercellular Apoptosis-indmentioning

confidence: 94%

Extracellular localization of catalase is associated with the transformed state of malignant cells

Böhm¹,

Heinzelmann²,

Motz³

et al. 2015

Biological Chemistry

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Oncogenic transformation is dependent on activated membrane-associated NADPH oxidase (NOX). However, the resultant extracellular superoxide anions are also driving the NO/peroxynitrite and the HOCl pathway, which eliminates NOX-expressing transformed cells through selective apoptosis induction. Tumor progression is dependent on dominant interference with intercellular apoptosis-inducing ROS signaling through membraneassociated catalase, which decomposes H 2 O 2 and peroxynitrite and oxidizes NO. Particularly, the decomposition of extracellular peroxynitrite strictly requires membraneassociated catalase. We utilized small interfering RNA (siRNA)-mediated knockdown of catalase and neutralizing antibodies directed against the enzyme in combination with challenging H 2 O 2 or peroxynitrite to determine activity and localization of catalase in cells from three distinct steps of multistage oncogenesis. Nontransformed cells did not generate extracellular superoxide anions and only showed intracellular catalase activity. Transformed cells showed superoxide anion-dependent intercellular apoptosis-inducing ROS signaling in the presence of suboptimal catalase activity in their membrane. Tumor cells exhibited tight control of intercellular apoptosis-inducing ROS signaling through a high local concentration of membrane-associated catalase. These data demonstrate that translocation of catalase to the outside of the cell membrane is already associated with the transformation step. A strong local increase in the concentration of membrane-associated catalase is achieved during tumor progression and is controlled by tumor cell-derived H 2 O 2 and by transglutaminase.

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SOD and catalase inactivation by singlet oxygen and peroxyl radicals

Cited by 305 publications

References 17 publications

Protective effect of bilobalide on learning and memory impairment in rats with vascular dementia

Protective effect of bilobalide on learning and memory impairment in rats with vascular dementia

Hepatic Histopathological Characteristics and Antioxidant Response of Phytoplanktivorous Silver Carp Intraperitoneally Injected with Extracted Microcystins

Extracellular localization of catalase is associated with the transformed state of malignant cells

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