SOD2 gene polymorphism may modulate biochemical responses to a 12-week swimming training

Jówko, Ewa; Gromisz, Wilhelm; Sadowski, Jerzy; Cieśliński, Igor; Kotowska, Jadwiga

doi:10.1016/j.freeradbiomed.2017.11.002

Cited by 6 publications

(7 citation statements)

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“…Namely, we noted that long-term (12-week) swimming training in untrained men reduced the level of lipid peroxidation and muscle cell damage. Importantly, these favorable changes induced by periodic swimming training seemed to depend on the Ala/Ala genotype, as this genotype was associated with a significant decrease in both serum concentration of lipid hydroperoxides and serum CK activity [40]. In addition, in our another study on athletes (wrestlers) [41], Ala/Ala genotype was related to the lowest (from all three genotypes within SOD2 Val16Ala polymorphism) level of serum hydroperoxides at rest.…”

Section: Discussionmentioning

confidence: 68%

“…Moreover, the level of the studied indicators varies individually (which we also observed in previous published studies), and it is possible that it may be influenced by lifestyle or dietary habits of the subjects, as well as other genetic factors apart from the examined. On the other hand, such a link was found in our previous studies on wrestlers [41] and untrained men undergoing long-term swimming training [40]. Therefore, it is most probable that SOD2 polymorphism can affect total SOD activity in erythrocytes, but only in exercise condition or in persons with high levels of adaptation to exercise (athletes).…”

Section: Discussionmentioning

confidence: 77%

“…There exists a lot of scientific evidence confirming beneficial effects of regular physical activity on oxidative-antioxidative status, decreasing the levels of free radical markers both at rest and after single intense physical exercise [38,39]. Moreover, our previous study revealed that the response of oxidative-antioxidative status to sports training may be modulated by SOD2 Val16Ala polymorphism [40]. Namely, we noted that long-term (12-week) swimming training in untrained men reduced the level of lipid peroxidation and muscle cell damage.…”

Section: Discussionmentioning

confidence: 83%

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Effect of Gene Polymorphisms in Antioxidant Enzymes on Oxidative-Antioxidative Status in Young Men

Kotowska

Jówko

2020

Polish Journal of Sport and Tourism

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Introduction. The aim of the study was to evaluate the relationship between SOD1 +35A/C, SOD2 Val16Ala and GPx1 Pro198Leu gene polymorphisms and baseline level of oxidative-antioxidative status in blood. Material and methods.The study included 154 male students of physical education who participated in practical classes included in the study curriculum. Genotyping was carried out on genomic DNA using real-time PCR reaction with TaqMan assays. Also, fasting blood samples were analyzed for biochemical parameters including superoxide dismutase (SOD) activity in erythrocytes and the activity of glutathione peroxidase (GPx) in whole blood, as well as serum concentration of lipid hydroperoxides (LOOHs) and total antioxidant capacity (TAC) of serum. Results. SOD2 polymorphism had a significant effect on serum LOOHs concentration. Individuals with Val/Val genotype presented a significantly higher level of LOOHs than Val/Ala genotype carriers (p < 0.05). In addition, no significant differences in SOD and GPx activity or TAC were found between SOD2 genotypes. Apart from the SOD2 polymorphism, no significant influence of both SOD1 and GPx1 polymorphisms on measured biochemical parameters was found, probably due to the lack of mutant homozygous genotypes in the study group. Conclusions. In young, healthy and physically active men, SOD2 polymorphism has an influence on the resting level of oxidative stress marker in the blood without affecting both enzymatic and non-enzymatic antioxidant defence. In turn, SOD1 and GPx1 polymorphisms do not seem to affect oxidative-antioxidative status. However, the absence of SOD1 CC and GPx1 Leu/Leu may indicate that these genotypes are disadvantageous, and thus underrepresented in young, healthy and physically fit population.

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Section: Discussionmentioning

confidence: 68%

Section: Discussionmentioning

confidence: 77%

Section: Discussionmentioning

confidence: 83%

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Effect of Gene Polymorphisms in Antioxidant Enzymes on Oxidative-Antioxidative Status in Young Men

Kotowska

Jówko

2020

Polish Journal of Sport and Tourism

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“…The training procedure was the same as previously described by Jówko et al [27]. The swimming training program lasted 12 weeks, with four sessions per week (Monday, Tuesday, Wednesday and Thursday; at 8.45 p.m.; 48 training sessions in total; 1.5 h/day).…”

Section: Training Proceduresmentioning

confidence: 99%

“…Their diagnostic potential as biomarkers for monitoring exercise and training has also been demonstrated [26]. Although physical activity is well known as a non-pharmacological strategy to enhance antioxidant defenses, resulting in reduced oxidative stress parameters [27], the relationship between eHSP70 levels, cytokines, and prooxidant-antioxidant status has not been previously described. Moreover, apart from the existing evidence of an association between HSP70 gene SNPs and various disease states, there is a lack of research on the effects of SNPs within HSP70 genes, along with IL-6 gene SNPs, on eHSP70, together with inflammation and prooxidant-antioxidant status and their response to exercise/training.…”

Section: Introductionmentioning

confidence: 99%

IL-6 and HSPA1A Gene Polymorphisms May Influence the Levels of the Inflammatory and Oxidative Stress Parameters and Their Response to a Chronic Swimming Training

Kotowska

Jówko

Cieśliński

et al. 2022

IJERPH

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The aim of the study was to evaluate whether the most common polymorphisms in the IL-6 and HSP70 genes affect the circulating heat shock protein 70 (HSP70), as well as inflammatory and prooxidant-antioxidant parameters in healthy men undergoing chronic endurance training. The subjects were randomly assigned to a 12-week swimming training (ST group) or control group (CON). Fasting blood samples were collected pre- and post-study period to assessment: superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities, serum levels of lipid hydroperoxides (LHs), tumor necrosis factor α (TNFα), and HSP70. Subjects were genotyped for IL-6-174G/C, HSPA1A + 190 G/C and HSPA1B + 1538 A/G single nucleotide polymorphisms (SNPs) by real-time PCR. After a 12-week study period, a decrease in TNFα, HSP70, and GPx was observed in the ST group, but not the CON group. IL-6 SNP affected serum TNFα levels (main effect of genotype). Higher TNFα levels (pre- and post-study period) was observed in CC CON than in other IL-6 genotypes of CON and ST groups. However, a post-training decrease in TNFα was observed in both GG and CC IL-6 genotypes of ST group. In turn, only GG IL-6 genotype of the ST group was related to a post-training decrease in HSP70 (main time and genotype interaction). Moreover, pre- and post-training LHs were lower in GG than GC/CC HSPA1A genotypes of the ST group (main genotype effect). In conclusion, polymorphisms within the IL-6 and HSPA1A genes seem to affect baseline levels of some inflammatory parameters and prooxidant-antioxidant status and/or their changes after chronic swimming training. However, the results should be confirmed in a study with a larger sample size, one that includes individuals with sedentary lifestyles.

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The serum biomarker chemerin promotes tumorigenesis and metastasis in oral squamous cell carcinoma

Liang

et al. 2019

Clinical Science

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Chemerin, which is encoded by retinoic acid receptor responder 2 (RARRES2), has been found to be related to malignant tumours, but its role in the development of oral squamous cell carcinoma (OSCC) is largely unexplored. In the present study, a higher serum level of chemerin was evident in patients with OSCC than in healthy individuals, and this high level of chemerin significantly decreased after tumour resection. In addition, high chemerin levels were positively associated with advanced tumour stage and lymph node metastasis. The expression levels of chemerin and Chemerin Receptor 23 (ChemR23) were positively correlated with the migration and invasion of OSCC cell lines. Recombinant chemerin (R-chemerin) enhanced the in vitro migration, invasion and proliferation of OSCC cells in a concentration-dependent manner, and short hairpin RNAs (shRNAs) targeting RARRES2 decreased chemerin expression and inhibited OSCC cell metastasis and proliferation both in vitro and in vivo. Additionally, R-chemerin activated manganese superoxide dismutase (SOD2) and increased the amount of intracellular hydrogen peroxide (H2O2), leading to a significant decrease in E-cadherin expression and dramatic increase in the expression of phosphorylated ERK1/2 (p-ERK1/2), Slug, Vimentin and N-cadherin, but shRNAs targeting RARRES2 reversed these effects. Moreover, knockdown of ChemR23 with small interfering RNAs (siRNA) significantly inhibited chemerin-induced OSCC cell migration/invasion and SOD2 activity. Our results revealed that chemerin is a novel biomarker for OSCC. Chemerin/ChemR23 promotes tumorigenesis and metastasis in OSCC and may be a new therapeutic target for OSCC.

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SOD2 gene polymorphism may modulate biochemical responses to a 12-week swimming training

Cited by 6 publications

References 46 publications

Effect of Gene Polymorphisms in Antioxidant Enzymes on Oxidative-Antioxidative Status in Young Men

Effect of Gene Polymorphisms in Antioxidant Enzymes on Oxidative-Antioxidative Status in Young Men

IL-6 and HSPA1A Gene Polymorphisms May Influence the Levels of the Inflammatory and Oxidative Stress Parameters and Their Response to a Chronic Swimming Training

The serum biomarker chemerin promotes tumorigenesis and metastasis in oral squamous cell carcinoma

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