Sodium acetate regulates milk fat synthesis through the activation of GPR41/GPR43 signaling pathway

Qi, Yingao; Zheng, Tenghui; Liu, Xinghong; Yang, Siwang; Li, Qihui; Shao, Jiayuan; Zeng, Xiangfang; Guan, Wutai; Zhang, Shihai

doi:10.3389/fnut.2023.1098715

Cited by 8 publications

(4 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…49 The provision of fatty acids to mammary glands is imperative for the synthesis of milk fat, 50 with acetate serving as the principal precursor for fat synthesis in milk. 51,52 This study hypothesizes that KGM increases the fat content of milk by increasing acetate in the circulatory system, which in turn increases the fat content of milk. The nutritional properties of human milk fats are advantageous for infants.…”

Section: Discussionmentioning

confidence: 99%

Maternal supplementation with konjac glucomannan improves maternal microbiota for healthier offspring during lactation

Gao,

Zhang,

Cao

et al. 2024

J Sci Food Agric

View full text Add to dashboard Cite

BACKGROUNDThe maternal diet during gestation and lactation affects the health of the offspring. Konjac glucomannan (KGM) is a significantly functional polysaccharide in food research, possessing both antioxidant and prebiotic properties. However, the mechanisms of how KGM regulates maternal nutrition remain insufficient and limited. This study aimed to investigate maternal supplementation with KGM during late gestation and lactation to benefit both maternal and offspring generations.RESULTSOur findings indicate that KGM improves serum low density lipoprotein cholesterol (LDL‐C) and antioxidant capacity. Furthermore, the KGM group displayed a significant increase in the feed intake‐related hormones neuropeptide tyrosine (NPY), Ghrelin, and adenosine monophosphate‐activated kinase (AMPK) levels. KGM modified the relative abundance of Clostridium, Candidatus Saccharimonas, unclassified Firmicutes, and unclassified Christensenellaceae in sow feces. Acetate, valerate, and isobutyrate were also improved in the feces of sows in the KGM group. These are potential target bacterial genera that may modulate the host's health. Furthermore, Spearman's correlation analysis unveiled significant correlations between the altered bacteria genus and feed intake‐related hormones. More importantly, KGM reduced interleukin‐6 (IL‐6) levels in milk, further improved IL‐10 levels, and reduced zonulin levels in the serum of offspring.CONCLUSIONIn conclusion, maternal dietary supplementation with KGM during late gestation and lactation improves maternal nutritional status by modifying maternal microbial and increasing lactation feed intake, which benefits the anti‐inflammatory capacity of the offspring serum.

show abstract

Section: Discussionmentioning

confidence: 99%

Maternal supplementation with konjac glucomannan improves maternal microbiota for healthier offspring during lactation

Gao,

Zhang,

Cao

et al. 2024

J Sci Food Agric

View full text Add to dashboard Cite

show abstract

“…Briefly, sodium acetate (Sigma, MO, U.S.A), sodium butyrate (Sigma, MO, U.S.A), sodium valerate (Ambeed, IL, U.S.A), and sodium chloride (NaCl; Sigma, Mo, U.S.A) were prepared at 200 mM concentration freshly in drinking water every two days. These concentrations were chosen based on earlier studies conducted in mice [ 26 , 27 ]. All SCFA and NaCl solutions were pH verified to control for acidity effects on daily consumption.…”

Section: Methodsmentioning

confidence: 99%

Short-chain-fatty acid valerate reduces voluntary alcohol intake in male mice

Bokoliya,

Russell,

Dorsett

et al. 2023

Preprint

View full text Add to dashboard Cite

Background Despite serious health and social consequences, effective intervention strategies for habitual alcohol binge drinking are lacking. Development of novel therapeutic and preventative approaches is highly desirable. Accumulating evidence in the past several years has established associations between the gut microbiome and microbial metabolites with drinking behavior, but druggable targets and their underlying mechanism of action are understudied. Results Here, using a drink-in-the-dark mouse model, we identified a microbiome metabolite-based novel treatment (sodium valerate) that can reduce excessive alcohol drinking. Sodium valerate is a sodium salt of valeric acidshort-chain-fatty-acid with similar structure as γ-aminobutyric acid (GABA). Ten days of oral sodium valerate supplementation attenuates excessive alcohol drinking by 40%, reduces blood ethanol concentration by 53%, and improves anxiety-like or approach-avoidance behavior in male mice, without affecting overall food and water intake. Mechanistically, sodium valerate supplementation increases GABA levels across stool, blood, and amygdala. It also significantly increases H4 acetylation in the amygdala of mice. Transcriptomics analysis of the amygdala revealed that sodium valerate supplementation led to changes in gene expression associated with functional pathways including potassium voltage-gated channels, inflammation, glutamate degradation, L-DOPA degradation, and psychological behaviors. 16S microbiome profiling showed that sodium valerate supplementation shifts the gut microbiome composition and decreases microbiome-derived neuroactive compounds through GABA degradation in the gut microbiome. Conclusion Our findings suggest that the sodium valerate holds promise as an innovative therapeutic avenue for the reduction of habitual binge drinking, potentially through multifaceted mechanisms.

show abstract

“…The animals were allowed to drink water and eat food freely. Mice were pregnant at 6 weeks of age and randomized as the following three groups ( n = 3 in each group): (i) mice fed normal drinking water (control group); (ii) mice (from pregnant day 14) fed acetate (200 mM) in drinking water for 14 days (acetate-treated group, this dosage of acetate was similar to that used to suppress lipolysis in previous studies); and (iii) mice (from pregnant day 14) fed acetate (200 mM) in drinking water for 14 days, followed by intraperitoneal injection with LY294002 (0.3 mg/kg body weight; dissolved in dimethyl sulfoxide; acetate + LY294002-treated group) . The control mice were administered the same volume of dimethyl sulfoxide intraperitoneally.…”

Section: Methodsmentioning

confidence: 99%

Acetate Upregulates GPR43 Expression and Function via PI3K-AKT-SP1 Signaling in Mammary Epithelial Cells during Milk Fat Synthesis

Yang,

Liu,

Zhang

et al. 2023

J. Agric. Food Chem.

View full text Add to dashboard Cite

This study investigated the mechanism underlying acetate-induced orphan G-protein-coupled receptor 43 (GPR43) expression and milk fat production. The mammary epithelial cells of dairy cows were treated with acetate, and the effects of GPR43 on acetate uptake and the expression of lipogenesis-related genes were determined by gas chromatography and quantitative polymerase chain reaction (qPCR), respectively. RNAi, inhibitor treatment, and luciferase assay were used to determine the effect of phosphoinositide 3-kinase-protein kinase B-specificity protein 1 (PI3K-AKT-SP1) signaling on acetate-induced GPR43 expression and function. The results showed that GPR43 was highly expressed in lactating cow mammary tissues, which was related to milk fat synthesis. 12 mM acetate significantly increased the GPR43 expression in mammary epithelial cells of dairy cows. In acetate-treated cells, GPR43 overexpression significantly increased the cellular uptake of acetate, the intracellular triacylglycerol (TAG) content, and acetate-induced lipogenesis gene expression. Acetate activated PI3K-AKT signaling and promoted SP1 translocation from the cytosol into the nucleus, where SP1 bound to the GPR43 promoter and upregulated GPR43 transcription. Moreover, the activation of PI3K-AKT-SP1 by acetate facilitated the trafficking of GPR43 from the cytosol to the plasma membrane. In conclusion, acetate upregulated GPR43 expression and function via PI3K-AKT-SP1 signaling in mammary epithelial cells, thereby increasing milk fat synthesis. These results provide an experimental strategy for improving milk lipid synthesis, which is important to the dairy industry.

show abstract

Sodium acetate regulates milk fat synthesis through the activation of GPR41/GPR43 signaling pathway

Cited by 8 publications

References 40 publications

Maternal supplementation with konjac glucomannan improves maternal microbiota for healthier offspring during lactation

Maternal supplementation with konjac glucomannan improves maternal microbiota for healthier offspring during lactation

Short-chain-fatty acid valerate reduces voluntary alcohol intake in male mice

Acetate Upregulates GPR43 Expression and Function via PI3K-AKT-SP1 Signaling in Mammary Epithelial Cells during Milk Fat Synthesis

Contact Info

Product

Resources

About