Sodium‐activated potassium current in mouse diaphragm

Re, Lamberto; Moretti, V.; Rossini, L; Giusti, Pietro

doi:10.1016/0014-5793(90)81266-q

Cited by 11 publications

(5 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the disappearance of the "A" type current, together with the fast inward current with the application of TTX led us to question the independence of this current. A similar behavior for the A-type currents has been observed in other systems using loose-patch clamp [44,48]. However, this dependence of the "A" component on the inward sodium current has not been observed in isolated terminals of the NH (see [53]).…”

Section: Discussionsupporting

confidence: 58%

Loose-patch clamp currents from the hypothalamo-neurohypophysial system of the rat

Marrero

Lemos

2003

Pflugers Arch - Eur J Physiol

View full text Add to dashboard Cite

The loose-patch clamp technique was used to study voltage-activated currents from the surface of rat neurohypophysial and hypothalamic regions in situ. In the neurohypophysis, depolarizing pulses of 4-8 ms duration yielded tetrodotoxin (TTX)-sensitive sodium currents, a 4-AP-sensitive "A"-type potassium current, and a long-lasting outward TEA- and tetrandrine-sensitive Ca(2+)-activated potassium current. All of these currents were elicited during the application of the pulse. With high external calcium there were long-lasting inward currents blocked by Ni(2+) and Cd(2+), identifying them as voltage-gated calcium currents. Depolarizing pulses of 0.3-0.7 ms duration yielded fast biphasic responses, of 1-3 ms duration, composed of mostly sodium and "A"-type potassium currents. With high external calcium there were fast inward currents blocked by Ni(2+) and Cd(2+), indicating that these were voltage-gated calcium currents. These responses have the characteristics of action potential currents: they were elicited after the cessation of the applied pulse and the "A" component is eliminated together with the sodium component upon application of TTX. Similar responses to long and short pulses were obtained from the surface of the associated magnocellular somata in the supraoptic nucleus, and their projections. The explant currents are similar to those previously characterized using conventional methods from somata and terminals.

show abstract

Section: Discussionsupporting

confidence: 58%

Loose-patch clamp currents from the hypothalamo-neurohypophysial system of the rat

Marrero

Lemos

2003

Pflugers Arch - Eur J Physiol

View full text Add to dashboard Cite

show abstract

“…They have also been found in guinea pig gastric myocytes [35] mouse diaphragm muscle [36], circular smooth muscle of the opossum lower esophageal sphincter [37], and the thick ascending limb of mouse kidney [38], as well as in Xenopus oocytes [39]. …”

Section: History Of Kna Channelsmentioning

confidence: 99%

Slack, Slick, and Sodium-Activated Potassium Channels

2013

View full text Add to dashboard Cite

The Slack and Slick genes encode potassium channels that are very widely expressed in the central nervous system. These channels are activated by elevations in intracellular sodium, such as those that occur during trains of one or more action potentials, or following activation of nonselective cationic neurotransmitter receptors such as AMPA receptors. This review covers the cellular and molecular properties of Slack and Slick channels and compares them with findings on the properties of sodium-activated potassium currents (termed KNa currents) in native neurons. Human mutations in Slack channels produce extremely severe defects in learning and development, suggesting that KNa channels play a central role in neuronal plasticity and intellectual function.

show abstract

“…More than 30 years ago, the existence of native sodium‐activated potassium currents was demonstrated through electrophysiological experiments in guinea pig cardiomyocytes (Kameyama et al, ). Since then, the presence of native sodium‐activated potassium channels has been reported in diverse myocytes (Kim et al, ; Re et al, ), pancreatic duct epithelial cells (Hayashi and Novak, ), thick ascending limb of Henle's loop in the kidney (Paulais et al, ), various mammalian neurons (Egan et al, ; Kaczmarek, ), and Xenopus oocytes (Egan et al, ). At present, two distinct ion channels giving rise to an outward rectifying potassium current activated upon rises in internal sodium ions have been described (Bhattacharjee and Kaczmarek, ; Salkoff et al, ; Yuan et al, ).…”

mentioning

confidence: 99%

Differential distribution of the sodium‐activated potassium channels slick and slack in mouse brain

Rizzi

Knaus

Schwarzer

2015

J of Comparative Neurology

View full text Add to dashboard Cite

The sodium‐activated potassium channels Slick (Slo2.1, KCNT2) and Slack (Slo2.2, KCNT1) are high‐conductance potassium channels of the Slo family. In neurons, Slick and Slack channels are involved in the generation of slow afterhyperpolarization, in the regulation of firing patterns, and in setting and stabilizing the resting membrane potential. The distribution and subcellular localization of Slick and Slack channels in the mouse brain have not yet been established in detail. The present study addresses this issue through in situ hybridization and immunohistochemistry. Both channels were widely distributed and exhibited distinct distribution patterns. However, in some brain regions, their expression overlapped. Intense Slick channel immunoreactivity was observed in processes, varicosities, and neuronal cell bodies of the olfactory bulb, granular zones of cortical regions, hippocampus, amygdala, lateral septal nuclei, certain hypothalamic and midbrain nuclei, and several regions of the brainstem. The Slack channel showed primarily a diffuse immunostaining pattern, and labeling of cell somata and processes was observed only occasionally. The highest Slack channel expression was detected in the olfactory bulb, lateral septal nuclei, basal ganglia, and distinct areas of the midbrain, brainstem, and cerebellar cortex. In addition, comparing our data obtained from mouse brain with a previously published study on rat brain revealed some differences in the expression and distribution of Slick and Slack channels in these species. J. Comp. Neurol. 524:2093–2116, 2016. © 2015 The Authors The Journal of Comparative Neurology Published by Wiley Periodicals, Inc.

show abstract

Sodium‐activated potassium current in mouse diaphragm

Cited by 11 publications

References 6 publications

Loose-patch clamp currents from the hypothalamo-neurohypophysial system of the rat

Loose-patch clamp currents from the hypothalamo-neurohypophysial system of the rat

Slack, Slick, and Sodium-Activated Potassium Channels

Differential distribution of the sodium‐activated potassium channels slick and slack in mouse brain

Contact Info

Product

Resources

About