Sodium and chloride co‐transport by preimplantation rabbit blastocysts.

Benos, D. J.; Biggers, John D.

doi:10.1113/jphysiol.1983.sp014837

Cited by 18 publications

(9 citation statements)

References 23 publications

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“…4), as a result of transtrophectodermal C efflux down the imposed C1-gradient and the resulting unquenching of the dye. This was also seen in the presence of SO 4 2 -as substitute anion. It was not possible to study both C1-exit and C1-re-entry without compromising the integrity of the blastocyst; therefore this C1-efflux was used to study the mechanism and regulation of transtrophectodermal Cl-transport.…”

Section: Measurement Of Ci-transport Using Spqmentioning

confidence: 52%

“…In the rabbit, 36 C1-uptake is unaffected by DIDS, but a furosemide-sensitive Na+/C1 -co-transporter is present in Day 6 blastocysts, although not in earlier ones [4]. Thus the rat blastocyst appears to share with the rabbit, but not the mouse, a transcellular pathway for Cl-transport.…”

Section: Pathways Of Ci-transport In the Rat Blastocystmentioning

confidence: 87%

“…The rabbit Na+/ C1-co-transporter is present only on Day 6, but not on Day 5 [4], and Ca 2 + channels are known to be present in early cleavage-stage mouse embryos, but disappear by the 8-16 cell stage [38,39].…”

Section: Pathways Of Ci-transport In the Rat Blastocystmentioning

confidence: 99%

“…Studies on the maximally expanding rabbit blastocyst have shown that active transport of Na + and Cl-is required for blastocoel cavity formation and that this is secondary to the activity of a ouabain-sensitive Na+,K+,ATPase [3]. Ions are thought to be taken up across the apical membrane by a variety of mechanisms including furosemide-sensitive Na+Cl -co-transport [4] and Na + channels [5], and extruded across the basolateral cell surface, in the case of Na + by the Na+,K+,ATPase. The movement of water follows passively.…”

Section: Introductionmentioning

confidence: 98%

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Role of Chloride Transport in the Development of the Rat Blastocyst1

Brison

Leese

1993

Biology of Reproduction

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Preimplantation mammalian development culminates in the generation of a fluid-filled cavity, the blastocoel, which requires the vectorial transport of ions across the trophectoderm, followed by the movement of water. Experiments were carried out to establish the role of Cl- transport in blastocoel formation in the rat. These included investigations of the effect of Cl- substitution and the Cl- transport inhibitors 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid, furosemide, and anthracene-9-carboxylic acid, on the development of morulae into blastocysts in culture, and on the rate at which blastocoel fluid is accumulated. In addition, a novel technique was developed in which the Cl(-)-sensitive fluorescent dye 6-methoxy-N-(3-sulfopropyl) quinolium (SPQ) was loaded into the blastocoel cavity, in order to characterize the pathways of transtrophectodermal Cl- transport. We established that 1) blastocoel formation in the rat requires the external presence of Cl- ions; 2) transport across the trophectoderm appears to be via a transcellular pathway, since rates of blastocyst development, fluid accumulation, and SPQ-monitored Cl- efflux are all reduced in the presence of Cl- transport inhibitors; and 3) Cl- transport occurs via Cl-/HCO3- exchange.

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Section: Measurement Of Ci-transport Using Spqmentioning

confidence: 52%

Section: Pathways Of Ci-transport In the Rat Blastocystmentioning

confidence: 87%

Section: Pathways Of Ci-transport In the Rat Blastocystmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

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Role of Chloride Transport in the Development of the Rat Blastocyst1

Brison

Leese

1993

Biology of Reproduction

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“…Cyclic AMP decreased the active transport of Na+ and Cl~in the blastocyst (Benos & Biggers, 1983). To determine whether a decrease in ion transport affected glucose metabolism to C02, we tested the effect of chlorophenylthio cyclic AMP (CPT cAMP; 10~4m) on C02 production.…”

Section: Methodsmentioning

confidence: 99%

Glucose metabolism in the trophectoderm and inner cell mass of the rabbit embryo

Robinson¹,

Benos²

1991

Reproduction

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Summary. The metabolism of glucose in the intact post coitum (p.c.) rabbit blastocyst and in the separated trophectoderm and inner cell mass (ICM) of the Day-7 p.c. embryo was investigated. At Day-6 p.c., glucose traversed the trophectoderm with a half-time of 39 \ m=+-\9\m=.\3 min, and was metabolized to CO2 at a rate of 25\m=.\5\m=+-\1\m=.\6 nmol \ m=. \ cm\ m=-\ 2\ m=. \ h\ m=-\ 1. Neither the Na+ ionophore, amphotericin B, nor cyclic AMP had an effect on glucose metabolism to CO2. Lactate production by the Day-6 blastocyst was largely independent of glucose. At Day-7 p.c. in the intact embryo, CO2 production from glucose significantly decreased to 7\m=.\76\ m=+-\ 2\m=.\8nmol\m=.\cm\m=-\2\m=.\h\m=-\1. Per unit surface area, the metabolism of glucose to CO2 was similar in the separated Day-7 p.c. trophectoderm and ICM. We conclude that the rabbit blastocyst is not highly dependent on glucose, and that the ICM does not utilize glucose as a metabolite to a greater extent than does the trophectoderm, at least in the Day-7 p.c. embryo.

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Effects of lloprost, a stable prostacyclin analog, PGE₂ and PGF_2α on rabbit blastocysts

Jones

Harper

1988

Gamete Research

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The uptake of prostaglandins (PGs) into day-6 rabbit blastocysts was studied. Uptake of PGE2 and PGF2 alpha was temperature sensitive and nonsaturable up to 1,000 nM external concentration. In contrast, uptake of Iloprost, a stable prostacyclin analog, was unaffected by temperature, and showed apparent saturation at greater than 2 nM concentration. Individual blastocysts exposed to 0.2 microM radioinert prostaglandins or 1 hr did not swell significantly. Blastocyst concentration of PGF2 alpha was 4.8 +/- 2.1-fold higher than that of PGE2, which was 9.5 +/- 2.4-fold greater than that of 6-keto-PGF1 alpha (the stable metabolite of prostacyclin). Uptake of tritiated water was increased by exposure of blastocysts to 0.2 microM PGE2 or PGF2 alpha, but not to Iloprost. Thus, imbibition of water does not cause blastocyst swelling at least during limited in vitro culture. It is concluded that prostacyclin is of less importance in blastocyst function than PGE2 or PGF2 alpha.

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Sodium and chloride co‐transport by preimplantation rabbit blastocysts.

Cited by 18 publications

References 23 publications

Role of Chloride Transport in the Development of the Rat Blastocyst1

Role of Chloride Transport in the Development of the Rat Blastocyst1

Glucose metabolism in the trophectoderm and inner cell mass of the rabbit embryo

Effects of lloprost, a stable prostacyclin analog, PGE₂ and PGF_2α on rabbit blastocysts

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Sodium and chloride co‐transport by preimplantation rabbit blastocysts.

Cited by 18 publications

References 23 publications

Role of Chloride Transport in the Development of the Rat Blastocyst1

Role of Chloride Transport in the Development of the Rat Blastocyst1

Glucose metabolism in the trophectoderm and inner cell mass of the rabbit embryo

Effects of lloprost, a stable prostacyclin analog, PGE2 and PGF2α on rabbit blastocysts

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Product

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Effects of lloprost, a stable prostacyclin analog, PGE₂ and PGF_2α on rabbit blastocysts