Sodium ascorbate as a quorum sensing inhibitor of<i>Pseudomonas aeruginosa</i>

El-Mowafy, Somaia A.; Shaaban, Mona I.; Galil, Khaled H. Abd El

doi:10.1111/jam.12631

Cited by 81 publications

(50 citation statements)

References 67 publications

(73 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several authors have already described the bacteriostatic activity of AA against Staphylococcus aureus, 18,19) as well as its synergy with some antibiotics. 20,21) Interesting results were obtained for Pseudomonas aeruginosa 22,23) alone and in the presence of an antibiotic 24) and for Candida albicans. [25][26][27] Escherichia coli seems to be inhibited in some conditions in the presence of AA.…”

Section: Discussionmentioning

confidence: 99%

Selected Physicochemical and Biological Properties of Ethyl Ascorbic Acid Compared to Ascorbic Acid

Golonka

Oleksy

Junka

et al. 2017

Biological & Pharmaceutical Bulletin

View full text Add to dashboard Cite

The aim of the work was the evaluation of selected biological and physicochemical, applicative properties of ethylated ascorbic acid (AAE) compared to ascorbic acid (AA). Thermogravimetry (TG), differential thermogravimetry (DTG), and differential thermal analysis (DTA) were conducted, followed by the evaluation of AAE decomposition by the UV-Vis spectroscopic method including the influence of temperature and pH. Scavenging, antimicrobial activity and cytotoxicity against L929 fibroblasts were also performed. The difference in mass loss between AA and AAE was 30% via TG. DTA revealed characteristic exothermic and endothermic effects. The AAE solution was more thermally stable than AA. The calculated zero-order rate constants of free-radical scavenging kinetics for AAE were in the range of 4.9 10 3 -1.35 10 2 s 1 . The activation energy for the process was 11,2281 kJ/mol. AAE was active against Staphylococcus (S.) aureus and Enterococcus (E.) faecalis and acted stronger against Candida (C.) albicans than AA. The concentrations of AA ≥2.5% were cytotoxic, whereas in the case of AAE, a 10% concentration was considered cytotoxic. DTG enables the detailed differentiation between AA and AAE. AAE in aqueous solution is more stable compared to AA. The antioxidant activity of AAE is significant. However, the reaction with 2,2-diphenyl-1-picrylhydrazyl (DPPH) indicates prolonged activity compared to AA. Variability in the antimicrobial activity of AAE may find practical application in the pharmaceutical and cosmetic industries. The potential for applicative aims may be supported by the relatively low in vitro toxicity of AAE.

show abstract

Section: Discussionmentioning

confidence: 99%

Selected Physicochemical and Biological Properties of Ethyl Ascorbic Acid Compared to Ascorbic Acid

Golonka

Oleksy

Junka

et al. 2017

Biological & Pharmaceutical Bulletin

View full text Add to dashboard Cite

show abstract

“…Average relative amounts of tested genes were then normalized to the average relative amount of the rpsL reference gene in the same sample. 35 In general (Figure 6), at a concentration of 400 μΜ ， resveratrol, piceatannol and oxyresveratrol down-regulated all of the tested genes, especially the lasI which was significantly decreased by approximately two-fold, five-fold and three-fold, respectively. In conclusion, we have conducted a screening study to evaluate the anti-QS activity of ten stilbenoids.…”

Section: Figure 1 Structures Of the Stilbenoids Used In This Studymentioning

confidence: 91%

The quorum-sensing inhibiting effects of stilbenoids and their potential structure–activity relationship

Sheng

Chen

Tan

et al. 2015

Bioorganic & Medicinal Chemistry Letters

View full text Add to dashboard Cite

“…The wells were stained with crystal violet for 15 min and the plates were washed with deionized water. The stained biofilm with crystal violet was dissolved with glacial acetic acid (33% v/v) and measured with an E max microtitre ELISA reader (Microplate reader, Per long Medical Equipment Co., Ltd. China) at OD 490 nm and the mean reading of three wells was calculated [27]. …”

Section: Methodsmentioning

confidence: 99%

Imipenem/cilastatin encapsulated polymeric nanoparticles for destroying carbapenem-resistant bacterial isolates

2017

View full text Add to dashboard Cite

BackgroundCarbapenem-resistance is an extremely growing medical threat in antibacterial therapy as the incurable resistant strains easily develop a multi-resistance action to other potent antimicrobial agents. Nonetheless, the protective delivery of current antibiotics using nano-carriers opens a tremendous approach in the antimicrobial therapy, allowing the nano-formulated antibiotics to beat these health threat pathogens. Herein, we encapsulated imipenem into biodegradable polymeric nanoparticles to destroy the imipenem-resistant bacteria and overcome the microbial adhesion and dissemination. Imipenem loaded poly Ɛ-caprolactone (PCL) and polylactide-co-glycolide (PLGA) nanocapsules were formulated using double emulsion evaporation method. The obtained nanocapsules were characterized for mean particle diameter, morphology, loading efficiency, and in vitro release. The in vitro antimicrobial and anti adhesion activities were evaluated against selected imipenem-resistant Klebsiella pneumoniae and Pseudomonas aeruginosa clinical isolates.ResultsThe obtained results reveal that imipenem loaded PCL nano-formulation enhances the microbial susceptibility and antimicrobial activity of imipenem. The imipenem loaded PCL nanoparticles caused faster microbial killing within 2–3 h compared to the imipenem loaded PLGA and free drug. Successfully, PCL nanocapsules were able to protect imipenem from enzymatic degradation by resistant isolates and prevent the emergence of the resistant colonies, as it lowered the mutation prevention concentration of free imipenem by twofolds. Moreover, the imipenem loaded PCL eliminated bacterial attachment and the biofilm assembly of P. aeruginosa and K. pneumoniae planktonic bacteria by 74 and 78.4%, respectively.ConclusionsThese promising results indicate that polymeric nanoparticles recover the efficacy of imipenem and can be considered as a new paradigm shift against multidrug-resistant isolates in treating severe bacterial infections.

show abstract

Sodium ascorbate as a quorum sensing inhibitor ofPseudomonas aeruginosa

Abstract: This study demonstrated that sodium ascorbate could function as signal modulator and virulence inhibitor in Ps. aeruginosa.

Cited by 81 publications

References 67 publications

Selected Physicochemical and Biological Properties of Ethyl Ascorbic Acid Compared to Ascorbic Acid

Selected Physicochemical and Biological Properties of Ethyl Ascorbic Acid Compared to Ascorbic Acid

The quorum-sensing inhibiting effects of stilbenoids and their potential structure–activity relationship

Imipenem/cilastatin encapsulated polymeric nanoparticles for destroying carbapenem-resistant bacterial isolates

Contact Info

Product

Resources

About