Sodium Azide Induced Morphological and Molecular Changes in Persimmon (Diospyros Lotus L.)

Abstract

Sodium Azide Induced Morphological and Molecular Changes in Persimmon (Diospyros LotusL.)PersimmonDiospyros lotusL. is an underutilized species, used as rootstock for Japanese persimmon (Diospyros kakiThunb.) in many European countries. It has a potential for ornamental uses due to its diploid genome that suggests an easy mutation induction. This study was aimed at unravelling the effects of mutagenic sodium azide (in concentrations of 0.1% and 0.5%) on morphological characters (plant height, maximal leaf blade length, maximal leaf blade width and leaf petiole length) and molecular relations of treated accessions, based on AFLP (amplified fragment length polymorphism). In the M1 generation, the analysed characters of two-year old plants differed among the treatments. Sodium azide influenced persimmon stem and leaves in the opposite way, with increase up to 31.57% in plant height and decrease up to 30.33% in leaf petiole length. Changes in morphological characters were significant, that implies the importance of azide influence on the persimmon. Most genotypes treated with 0.1% azide were without significant differences among them and clustered together. The results suggest that the higher azide concentration of 0.5% is more suitable for persimmon seeds azide treatment to form genotypes, significantly different from the control, at the molecular level.

Enhancement drought tolerance of sugar beet (Beta vulgaris L.) in vitro by sodium azide

“…In addition to, Vwioko et al (2019) stated that NaN3 promote plant height and number of leaves in okra plants under stress. Also, Kochanová et al (2012) and Al-Qurainy (2009) demonstrated that sodium azide could stimulate the growth and height of Eruca sativa and Diospyros lotus, respectively. Moreover, the application of sodium azide treatments can help elucidate the impact of drought stress on photosynthetic pigments in maize (Hamideldin & Eliwa, 2015).…”

Stress-Generated Free Radicals Detected by Electron Paramagnetic Resonance Spectroscopy with Nitrone Spin Trap in Vicia faba Root

“…Sodium azide (NaN3) has been found to be mutagenic to various bacterial strains, plants, and animals, and cause stress when used in an excess amount. Increased genetic variability, enhanced stress tolerance, and crop yields in crops (e.g., barley, wheat, groundnut, tomato, chickpea) have been shown to be induced by NaN3 treatment [9][10][11][12][13][14]. L-Azidoalanine, formed by the action of the enzyme lyase on Oacetylserine and azide is responsible for the mutagenic effects (point mutation) induced by NaN3 [15][16][17].…”