Sodium butyrate improved performance while modulating the cecal microbiota and regulating the expression of intestinal immune-related genes of broiler chickens

Bortoluzzi, Cristiano; Pedroso, Adriana Ayres; Mallo, J. J.; Puyalto, Mónica; Kim, W K; Applegate, T. J.

doi:10.3382/ps/pex218

Cited by 89 publications

(88 citation statements)

References 81 publications

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“…To further investigate the mechanism underlying BLJ mitigating NE-induced gut injury, the cecal microbiota structure was analyzed via Illumina MiSeq sequencing. This study revealed no differences in α-diversity of the cecal microbiota between the four experimental groups, which was consistent with previous results [67]. In addition, principal component analysis showed that BLJ supplementation, NE challenge or both altered the β-diversity of the cecal microbiota, indicating that these treatments significantly affected the intestinal bacterial community profiles.…”

Section: Discussionsupporting

confidence: 92%

“…In addition, principal component analysis showed that BLJ supplementation, NE challenge or both altered the β-diversity of the cecal microbiota, indicating that these treatments significantly affected the intestinal bacterial community profiles. However, NE challenge reduced the relative abundance of the phylum Firmicutes (67.65% vs. 82.27%) and increased the relative levels of Bacteroidetes compared with those of the unchallenged group, which helps explain the impaired BWG in the NE-infected birds, consistent with previous research [67]. We also found that the relative abundance of Firmicutes increased, while the relative abundance of Bacteroidetes decreased after BLJ treatment in unchallenged birds.…”

Section: Discussionsupporting

confidence: 90%

“…We also found that the relative abundance of Firmicutes increased, while the relative abundance of Bacteroidetes decreased after BLJ treatment in unchallenged birds. Increases in fecal Firmicutes have been associated with increased nutrient absorption [68] and body weight gain [69], whereas increases in fecal Bacteroidetes have been associated with decreased nutrient absorption [67,70]. Therefore, a higher abundance of Firmicutes might enhance energy absorption in birds fed BLJ, resulting in the improved FCR observed in our study.…”

Section: Discussionmentioning

confidence: 50%

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Dietary encapsulated essential oils and organic acids mixture improves gut health in broiler chickens challenged with necrotic enteritis

Pham

Kan

Huang³

et al. 2020

J Animal Sci Biotechnol

105

101

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Background: The poultry industry is in need of effective antibiotic alternatives to control outbreaks of necrotic enteritis (NE) due to Clostridium perfringens. In the present study, we investigated the effects of dietary supplementation with a blend of encapsulated essential oils and organic acids (BLJ) on growth performance and gut health using a coinfection model of NE in broiler chickens. Methods: Two hundred and eighty-eight one-day-old male Arbor Acres broiler chicks were randomly assigned using a 2 × 2 factorial design into two groups fed either 0 or 500 mg/kg dietary BLJ and co-challenged (or not challenged for the control) with Eimeria spp./C. perfringens. Results: Infected birds fed the BLJ-supplemented diet exhibited an improved feed conversion ratio throughout the trial (P < 0.01), a higher villus height and villus height/crypt depth ratio, and reduced intestinal C. perfringens counts, liver C. perfringens carriage, gut lesion scores and serum fluorescein isothiocyanate dextran (FITC-D) concentrations at 7 d postinfection compared with those of birds without BLJ supplementation (P < 0.05). NE-infected birds fed BLJ exhibited significantly upregulated claudin-1 and IGF-2 mRNA levels (P < 0.05), increased A20 mRNA expression and significantly downregulated TRAF-6, TNFSF15 and TOLLIP mRNA levels in the jejunum at 7 d post-infection compared with those in birds without BLJ supplementation (P < 0.05). Compared with the uninfected and untreated birds, the uninfected birds fed BLJ displayed increased relative abundances of Lactobacillus and Coprococcus but reduced Rikenellaceae levels. Compared with the unsupplemented NE-challenged birds, infected birds fed BLJ showed an increased relative abundance of Unclassified_ Lachnospiraceae and a significantly decreased relative abundance of Erysipelotrichaceae. Conclusion: BLJ supplementation improved growth performance and gut health in NE-infected broiler chickens by strengthening the intestinal barrier function, positively modulating the gut microbiota community and differentially regulating intestinal immune responses. Our results also suggested that adding BLJ effectively controlled NE infections after experimental Eimeria and Clostridium perfringens coinfection.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 50%

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Dietary encapsulated essential oils and organic acids mixture improves gut health in broiler chickens challenged with necrotic enteritis

Pham

Kan

Huang³

et al. 2020

J Animal Sci Biotechnol

105

101

View full text Add to dashboard Cite

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“…Odoribacter spp. decreases in cecal communities have been associated with butyric acid supplementation in chicken diets (Bortoluzzi et al, 2017). Together, these observations suggest that increases in abundance and/or activity of butyrate-producing taxa, such as Faecalibacterium and Butyricicoccus spp., may in fact dictate community dynamics and host-microbiome activities by generating fermentative metabolites and perhaps influence phenotypes later in life.…”

Section: Differentially Abundant Lineagesmentioning

confidence: 92%

Broiler Chickens and Early Life Programming: Microbiome transplant-induced cecal bacteriome dynamics and phenotypic effects

Ramírez¹,

Richardson²,

Clark³

et al. 2020

Preprint

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The concept of successional trajectories describes how small differences in initial community composition can magnify through time and lead to significant differences in mature communities. For many animals, the types and sources of early-life exposures to microbes have been shown to have significant and long-lasting effects on the community structure and/or function of the microbiome. In modern commercial poultry production, chicks are reared as a single age cohort and do not directly encounter adult birds. This scenario is likely to initiate a trajectory of microbial community development that is significantly different than non-industrial settings where chicks are exposed to a much broader range of environmental and fecal inocula; however, the comparative effects of these two scenarios on microbiome development and function remain largely unknown. In this work, we performed serial transfers of cecal material through multiple generations of birds to first derive a stable source of inoculum. Subsequently, we compared microbiome development between chicks receiving this passaged cecal material, versus an environmental inoculum, to test the hypothesis that the first exposure of newly hatched chicks to microbes determines early GI microbiome structure and may have longer-lasting effects on bird health and development. Cecal microbiome dynamics and bird weights were tracked for a two-week period, with half of the birds in each treatment group exposed to a pathogen challenge at 7d of age. We report that: i) a relatively stable community was derived after a single passage of transplanted cecal material, ii) this cecal inoculum significantly but ephemerally altered community structure relative to the environmental inoculum and PBS controls, and iii) either microbiome transplant administered at day-of-hatch appeared to have some protective effects against pathogen challenge relative to uninoculated controls. Differentially abundant taxa were identified across treatment types and may inform future studies and identify strains associated with beneficial phenotypes.

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“…Accumulating evidences suggest that the intestinal microbiota could affect the poultry production [3].…”

Section: Introductionmentioning

confidence: 99%

Characterization of the Intestinal Microbiota and Metabolites of Broiler Breeders with Different Egg Laying Rate

Yang¹,

Wang²,

Celi³

et al. 2019

Preprint

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BackgroundThe aim of the present study was to determine differences in the intestinal microbiota and metabolites of broiler breeders with different egg laying rate; and its possible relationships between intestinal microbiota and intestinal metabolites were also explored.MethodsA total of 200 AA+ parent broiler breeders (41-week-old) were separated into two groups according to their different egg laying rate: average reproductive group (AR: 77.26%±0.88%) and high reproductive group (HR: 86.67%±0.75%), with 10 replicates and 10 birds each 42-days study.ResultsFeed conversion ratio (FCR), ovary cell apoptosis rate (ApoCR) and abdominal fat pad weight percentage were lower (p < 0.05), while the hatchability of qualified egg rate (HQR) was higher (p < 0.05) in HR group than that in AR group. At phylum level, Firmicutes and Proteobacteria were the dominant bacteria in small intestines, while Bacteroidetes was dominant bacteria in cecum. At genus level, Lactobacillus was dominant in small intestines while Bacteroides were dominant in cecum. In HR Phascolarctobacterium were lower (p < 0.05) in ileum. A positive correlation was observed between Actinobacteria in the small intestines and ApoCR (r = 0.34 & p < 0.05). A positive correlation was observed between FCR and Spirochaetes in cecum (r = 0.54 & p < 0.05). The metabolomics analysis on ileal digesta content revealed a higher concentration of Octadecanoic acid, 9,12-Octadecadienoic acid, 11-trans-Octadecenoic acid, d-Ribose and Inositol in HR broiler breeders. Moreover, inositol was enriched in "Phosphatidylinositol signaling system", "Inositol phosphate metabolism", "Ascorbate and aldarate metabolism", "Galactose metabolism", "ABC transporters", and Octadecanoic acid was enriched in "Fatty acid biosynthesis", "Biosynthesis of unsaturated fatty acids".ConclusionsOur results suggest that glycerol, inositol modulate energy partitioning in fat metabolism to influence reproductive performances. Microbiota, such as Actinobacteria (phylum), Spirochaetes (phylum), and Phascolarctobacterium (genus) may have negative relationship with broiler breeders’ reproductive performances.

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Sodium butyrate improved performance while modulating the cecal microbiota and regulating the expression of intestinal immune-related genes of broiler chickens

Cited by 89 publications

References 81 publications

Dietary encapsulated essential oils and organic acids mixture improves gut health in broiler chickens challenged with necrotic enteritis

Dietary encapsulated essential oils and organic acids mixture improves gut health in broiler chickens challenged with necrotic enteritis

Broiler Chickens and Early Life Programming: Microbiome transplant-induced cecal bacteriome dynamics and phenotypic effects

Characterization of the Intestinal Microbiota and Metabolites of Broiler Breeders with Different Egg Laying Rate

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