Sodium butyrate inhibits migration and induces AMPK‐mTOR pathway‐dependent autophagy and ROS‐mediated apoptosis via the miR‐139‐5p/Bmi‐1 axis in human bladder cancer cells

Wang, Feifan; Wu, Huitao; Fan, Mengjing; Yu, Rikao; Zhang, Yan; Liu, Jiaxin; Zhou, Xinping; Cai, Yueshu; Huang, Song; Hu, Zhenghui; Jin, Xiaodong

doi:10.1096/fj.201902626r

Cited by 82 publications

(58 citation statements)

References 59 publications

(198 reference statements)

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“…As a consequence the viability of cancer cells was reduced and the process of caspase-dependent apoptosis was activated. MiR-139-5p targets Bmi-1 which induces reversion of invasion and migration [ 76 ]. Hydroxycamptothecinals initiated apoptosis via promoting AMPK/mTOR pathway dependent apoptosis [ 77 ], but the exact involvement of miRNAs in this process is not yet known.…”

Section: Mirna-mediated Regulation Of Mtor In Bladder Cancermentioning

confidence: 99%

Resveratrol, curcumin, paclitaxel and miRNAs mediated regulation of PI3K/Akt/mTOR pathway: go four better to treat bladder cancer

et al. 2020

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Bladder cancer (BC) is a leading cause of death among urothelial malignancies that more commonly affect male population. Poor prognosis and resistance to chemotherapy are the two most important characteristics of this disease. PI3K/Akt/mTOR signaling pathway has been considered pivotal in the regulation of proliferation, migration, invasiveness, and metastasis. Deregulation of PI3K/Akt/mTOR signaling has been found in 40% of bladder cancers. Several microRNAs (miRNAs) have been reported to interact with the PI3K/Akt/mTOR signaling pathway with a different possible role in proliferation and apoptosis in bladder cancer. Thus, miRNAs can be used as potential biomarkers for BC. Natural compounds have been in the spotlight for the past decade due to their effective anti-proliferative capabilities. However, little is known of its possible effects in bladder cancer. The aim of this review is to discuss the interplay between PI3K/Akt/mTOR, miRNAs, and natural compounds and emphasize the importance of miRNAs as biomarkers and resveratrol, curcumin and paclitaxel as a possible therapeutic approach against bladder cancer.

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Section: Mirna-mediated Regulation Of Mtor In Bladder Cancermentioning

confidence: 99%

Resveratrol, curcumin, paclitaxel and miRNAs mediated regulation of PI3K/Akt/mTOR pathway: go four better to treat bladder cancer

et al. 2020

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“…Of note, the modulated role of TEAD depends on binding with TAZ or YAP in nucleus whose nuclear importation is mediated by LATS1/2 16,17,44 . MiRNAs participate in numerous biological processes, including tumor initiation, progression and metastasis 24,[32][33][34]59 . Nevertheless, the underlying mechanism of dysregulated miRNAs in bladder cancer remains unclear.…”

Section: Discussionmentioning

confidence: 99%

“…Other in vitro experiments CCK-8 assay, colony formation assay and HE staining were performed according to previously described methods 24 .…”

Section: Glycolysis Process Evaluationmentioning

confidence: 99%

“…However, EMT is associated with tumorigenesis and metastasis and is unintentional in cancer progression [18][19][20] . Therefore, the EMT-related signaling pathways have been studied as a novel focus for cancer therapy in past decades [21][22][23][24] . Interestingly, emerging studies have demonstrated the regulatory role of the components in Hippo signaling pathway in EMT process [25][26][27][28][29] .…”

Section: Introductionmentioning

confidence: 99%

“…MicroRNAs regulate the level of protein-coding genes by binding to the speci c sequence of mRNAs 30 . A growing number of researches have reported that microRNAs involve in multiple aspects of biologic cellular processes, including cancer development and progression, making them novel therapeutic targets for treatment 24,31,32 . Moreover, the dysregulation of miRNAs and their in uences on tumorigenesis, development, and progression have been discovered in bladder cancer 33,34 .…”

Section: Introductionmentioning

confidence: 99%

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A positive feedback loop between TAZ and miR-942-3p modulates proliferation, migration, epithelial-mesenchymal transition process and glycometabolism in human bladder cancer

Wang

Fan

Zhou

et al. 2020

Preprint

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Background: Transcriptional co-activator with PDZ-binding motif (TAZ) has been reported to involve in tumor progression, epithelial-mesenchymal transition (EMT) process and glycometabolism modulation. Herein, the underlying molecular mechanisms of TAZ-induced biological effects in bladder cancer were discovered; Methods: qRT-PCR, western blot and immunohistochemistry were performed to determine the level of TAZ in bladder cancer cells and tissues; CCK-8 assay, Colony formation assay, wound healing assay and Transwell assay were performed to evaluate the functions of TAZ, miR-942-3p and GAS1. qRT-PCR and western blot were used to determine the expression levels of related genes. Chromatin immunoprecipitation and dual-luciferase reporter assay confirmed the interaction between TAZ and miR-942. In vivo tumorigenesis assay and colorimetric assay of glycolysis were also conducted; Results: We determined the upregulation and vital roles of TAZ in bladder cancer. TAZ-induced upregulation of miR-942-3p amplified upstream signaling by inhibiting the expression of large tumor suppressor 2 (LATS2, a TAZ inhibitor). MiR-942-3p attenuated the suppression of cell proliferation, EMT process and glycolysis induced by TAZ knockdown. Further, miR-942-3p resulted in restrained expression of growth arrest-specific 1 (GAS1) to modulate biological functions; Conclusion: Our study identified a novel positive feedback loop between TAZ and miR-942-3p that regulates biological functions in bladder cancer cells via GAS1 expression, and illustrated that TAZ and miR-942-3p might be potential therapeutic targets for bladder cancer treatment.

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Butyrate prevents the migration and invasion, and aerobic glycolysis in gastric cancer via inhibiting Wnt/β‐catenin/c‐Myc signaling

Liang

Rao

et al. 2023

Drug Development Research

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Gastric cancer (GC) remains a common cause of cancer death worldwide. Evidence has found that butyrate exhibited antitumor effects on GC cells. However, the mechanism by which butyrate regulate GC cell proliferation, migration, invasion, and aerobic glycolysis remains largely unknown. The proliferation, migration, and invasion of GC cells were tested by EdU staining, transwell assays. Additionally, protein expressions were determined by western blot assay. Next, glucose uptake, lactate production, and cellular ATP levels in GC cells were detected. Furthermore, the antitumor effects of butyrate in tumor-bearing nude mice were evaluated.We found, butyrate significantly prevented GC cell proliferation, migration, and invasion (p < .01). Additionally, butyrate markedly inhibited GC cell aerobic glycolysis, as shown by the reduced expressions of GLUT1, HK2, and LDHA (p < .01). Moreover, butyrate notably decreased nuclear β-catenin and c-Myc levels in GC cells (p < .01). Remarkably, through activating Wnt/β-catenin signaling with LiCl, the inhibitory effects of butyrate on the growth and aerobic glycolysis of GC cells were diminished (p < .01). Moreover, butyrate notably suppressed tumor volume and weight in GC cell xenograft nude mice in vivo (p < .01). Meanwhile, butyrate obviously reduced nuclear β-catenin, c-Myc, GLUT1, HK2 and LDHA levels in tumor tissues in GC cell xenograft mice (p < .01). Collectively, butyrate could suppress the growth and aerobic glycolysis of GC cells in vitro and in vivo via downregulating wnt/β-catenin/c-Myc signaling. These findings are likely to prove useful in better understanding the role of butyrate in GC.

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Sodium butyrate inhibits migration and induces AMPK‐mTOR pathway‐dependent autophagy and ROS‐mediated apoptosis via the miR‐139‐5p/Bmi‐1 axis in human bladder cancer cells

Cited by 82 publications

References 59 publications

Resveratrol, curcumin, paclitaxel and miRNAs mediated regulation of PI3K/Akt/mTOR pathway: go four better to treat bladder cancer

Resveratrol, curcumin, paclitaxel and miRNAs mediated regulation of PI3K/Akt/mTOR pathway: go four better to treat bladder cancer

A positive feedback loop between TAZ and miR-942-3p modulates proliferation, migration, epithelial-mesenchymal transition process and glycometabolism in human bladder cancer

Butyrate prevents the migration and invasion, and aerobic glycolysis in gastric cancer via inhibiting Wnt/β‐catenin/c‐Myc signaling

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