Sodium butyrate protects against rotavirus-induced intestinal epithelial barrier damage by activating AMPK-Nrf2 signaling pathway in IPEC-J2 cells

Xia, Dong; Wang, Yi; Zhu, Xiaoxiao; Shen, Linyuan; Chen, Lei; Niu, Lili; Gan, Mailin; Zhang, Shunhua; Zhang, Ming; Jiang, Jun; Chen, Shuai; Zhu, Li; Zhao, Ye

doi:10.1016/j.ijbiomac.2022.12.219

Cited by 11 publications

(5 citation statements)

References 64 publications

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“…TF has been reported to activate the AMPK signaling pathway, and this activated signaling pathway has an inhibitory effect on virus proliferation ( Dong et al., 2023 ; Qu et al., 2021 ). This guided us to explore whether TF inhibits ASFV replication by regulating the AMPK signaling pathway.…”

Section: Resultsmentioning

confidence: 99%

Theaflavin inhibits African swine fever virus replication by disrupting lipid metabolism through activation of the AMPK signaling pathway in virto

et al. 2023

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Section: Resultsmentioning

confidence: 99%

Theaflavin inhibits African swine fever virus replication by disrupting lipid metabolism through activation of the AMPK signaling pathway in virto

et al. 2023

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“…Previous studies have found that NaB can relieve sepsis‐related brain injury by GPR109A/Nrf2/HO‐1 pathway 60 . NaB can restore intestinal mucosal mechanical barrier function by activating the GPR109A/AMPK/Nrf2 signal pathway 61 . Nrf2 is a crucial transcription factor that regulates the endogenous ROS clearance system.…”

Section: Discussionmentioning

confidence: 99%

“…59 Previous studies have found that NaB can relieve sepsisrelated brain injury by GPR109A/Nrf2/HO-1 pathway. 60 NaB can 61 Nrf2 is a crucial transcription factor that regulates the endogenous ROS clearance system. Nrf2 has been called the "master regulator" of the antioxidant response because it modulates the expression of hundreds of genes, including not only antioxidant enzymes but also many genes that control multiple biological processes, such as immune and inflammatory responses.…”

Section: Blockade Of Nrf2/ho-1 Signaling Abolished the Anti-inflammat...mentioning

confidence: 99%

Sodium butyrate alleviates experimental autoimmune prostatitis by inhibiting oxidative stress and NLRP3 inflammasome activation via the Nrf2/HO‐1 pathway

Hua,

Zhang,

Chen

et al. 2024

The Prostate

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BackgroundChronic prostatitis and chronic pelvic pain syndrome (CP/CPPS) leads to severe discomfort in males and loss of sperm quality. Current therapeutic options have failed to achieve satisfactory results. Sodium butyrate (NaB) plays a beneficial role in reducing inflammation, increasing antioxidant capacities, and improving organ dysfunction; additionally NaB has good safety prospects and great potential for clinical application. The purpose of the current research was to study the effect of NaB on CP/CPPS and the underlying mechanisms using a mouse model of experimental autoimmune prostatitis (EAP) mice.MethodsThe EAP mouse model was successfully established by subcutaneously injecting a mixture of prostate antigen and complete Freund's adjuvant. Then, EAP mice received daily intraperitoneal injections of NaB (100, 200, or 400 mg/kg/day) for 16 days, from Days 26 to 42. We then explored anti‐inflammatory potential mechanisms of NaB by studying the effects of Nrf2 inhibitor ML385 and HO‐1 inhibitor zinc protoporphyrin on prostate inflammation and pelvic pain using this model. On Day 42, hematoxylin‐eosin staining and dihydroethidium staining were used to evaluate the histological changes and oxidative stress levels of prostate tissues. Chronic pelvic pain was assessed by applying Von Frey filaments to the lower abdomen. The levels of inflammation‐related cytokines, such as interleukin (IL)−1β, IL‐6, and tumor necrosis factor were detected by enzyme‐linked immunosorbent assay. The regulation of Nrf2/HO‐1 signaling pathway and the expression of NLRP3 inflammasome‐related protein in EAP mice were detected by western blot analysis assay.ResultsCompared with the EAP group, chronic pain development, histological manifestations, and cytokine levels showed that NaB reduced the severity of EAP. NaB treatment could inhibit NLRP3 inflammasome activation. Mechanism studies showed that NaB intervention could alleviate oxidative stress in EAP mice through Nrf2/HO‐1 signal pathway. Nrf2/HO‐1 pathway inhibitors can inhibit NaB ‐mediated oxidative stress. The inhibitory effect of NaB on the activation of NLRP3 inflammasome and anti‐inflammatory effect can also be blocked by Nrf2/HO‐1 pathway.ConclusionsNaB treatment can alleviates prostatic inflammation and pelvic pain associated with EAP by inhibiting oxidative stress and NLRP3 inflammasome activation via the Nrf2/HO‐1 pathway. NaB has the potential as an effective agent in the treatment of EAP.

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“…It is common knowledge that antioxidant enzymes form the rst line of defense against free radical damage to organisms. [38,39] Therefore, we investigated the activities of antioxidative enzymes, including antioxidases catalase (CAT), glutathione peroxidase (GPX) and superoxide dismutase (SOD). As illustrated in Fig.…”

Section: In Vitro Radioprotective Effectmentioning

confidence: 99%

Oral Administration of Probiotic Spore Ghosts for Efficient Attenuation of Radiation-Induced Intestinal Injury

Zheng,

Niu,

Kong

et al. 2024

Preprint

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Radiation-induced intestinal injury is the most common side effect during radiotherapy of abdominal or pelvic solid tumors significantly impacting patients’ quality of life and even resulting in poor prognosis. Until now, oral application of conventional formulations for intestinal radioprotection remains challenging with no preferred method available to mitigate radiation toxicity in small intestine. Our previous study revealed that nanomaterials derived from spore coat of probiotics exhibit superior anti-inflammatory effect and even prevent the progression of cancer. The aim of this work is to determine the radioprotective effect of spore coat (denoted as spore ghosts, SGs) from three clinically approved probiotics (B.coagulans, B.subtilis and B.licheniformis). All the three SGs exhibit outstanding reactive oxygen species (ROS) scavenging ability and excellent anti-inflammatory effect. Moreover, these SGs can reverse the balance of intestinal flora by inhibiting harmful bacteria and increasing the abundance of Lactobacillus. Consequently, administration of SGs significantly reduce radiation-induce intestinal injury by alleviating diarrhea, preventing X-ray induced apoptosis of small intestinal crypt epithelial cells and promoting restoration of barrier integrity in a prophylactic study. Notably, SGs markedly improve weight gain and survival of mice received total abdominal X-ray radiation. This work may provide promising radioprotectants for efficiently attenuating radiation-induced gastrointestinal syndrome and promote the development of new intestinal predilection.

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Sodium butyrate protects against rotavirus-induced intestinal epithelial barrier damage by activating AMPK-Nrf2 signaling pathway in IPEC-J2 cells

Cited by 11 publications

References 64 publications

Theaflavin inhibits African swine fever virus replication by disrupting lipid metabolism through activation of the AMPK signaling pathway in virto

Theaflavin inhibits African swine fever virus replication by disrupting lipid metabolism through activation of the AMPK signaling pathway in virto

Sodium butyrate alleviates experimental autoimmune prostatitis by inhibiting oxidative stress and NLRP3 inflammasome activation via the Nrf2/HO‐1 pathway

Oral Administration of Probiotic Spore Ghosts for Efficient Attenuation of Radiation-Induced Intestinal Injury

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