Sodium Butyrate Reduces Salmonella Enteritidis Infection of Chicken Enterocytes and Expression of Inflammatory Host Genes in vitro

Gupta, Anamika; Bansal, Mohit; Wagle, Basanta R.; Sun, Xiaolun; Rath, N. C.; Donoghue, Annie; Upadhyay, Abhinav

doi:10.3389/fmicb.2020.553670

Cited by 25 publications

(21 citation statements)

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“…The SIC of sodium butyrate against S. Enteritidis was determined based on growth curve analysis. The three concentrations of sodium butyrate that did not reduce S. Enteritidis growth after 12 h of incubation at 37˚C were 11, 22 and 45 mM [21]. Therefore, we have selected the highest SIC 45 mM of sodium butyrate to culture HTC cells and study the global protein expression by proteomic assay.…”

Section: Sic Of Sodium Butyrate Against S Enteritidismentioning

confidence: 99%

“…VIM released by activated macrophages promotes production of oxidative metabolites and bacterial killing in response to pro-inflammatory signaling pathways [56]. Salmonella infection in chicken macrophages promotes pro-inflammatory cytokine immune response for its invasion and survival [21]. It is necessary to investigate whether sodium butyrate-upregulated VIM protein activates inflammatory response.…”

Section: Plos Onementioning

confidence: 99%

“…SCFA butyric acid has been Generally Recognised as Safe (GRAS) antimicrobials for use in foods (Butyric acid-21CFR182.60) [20]. We have recently found that sodium butyrate effectively reduced S. Enteritidis attachment and invasion into the primary chicken enterocytes [21]. We also reported that sodium butyrate reduced S. Enteritidis invasion and inflammatory genes (Il1β, Il8 and Mmp9) expression in chicken macrophages (HTC cells).…”

Section: Introductionmentioning

confidence: 99%

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Sodium butyrate modulates chicken macrophage proteins essential for Salmonella Enteritidis invasion

et al. 2021

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Salmonella Enteritidis is an intracellular foodborne pathogen that has developed multiple mechanisms to alter poultry intestinal physiology and infect the gut. Short chain fatty acid butyrate is derived from microbiota metabolic activities, and it maintains gut homeostasis. There is limited understanding on the interaction between S. Enteritidis infection, butyrate, and host intestinal response. To fill this knowledge gap, chicken macrophages (also known as HTC cells) were infected with S. Enteritidis, treated with sodium butyrate, and proteomic analysis was performed. A growth curve assay was conducted to determine sub-inhibitory concentration (SIC, concentration that do not affect bacterial growth compared to control) of sodium butyrate against S. Enteritidis. HTC cells were infected with S. Enteritidis in the presence and absence of SIC of sodium butyrate. The proteins were extracted and analyzed by tandem mass spectrometry. Our results showed that the SIC was 45 mM. Notably, S. Enteritidis-infected HTC cells upregulated macrophage proteins involved in ATP synthesis through oxidative phosphorylation such as ATP synthase subunit alpha (ATP5A1), ATP synthase subunit d, mitochondrial (ATP5PD) and cellular apoptosis such as Cytochrome-c (CYC). Furthermore, sodium butyrate influenced S. Enteritidis-infected HTC cells by reducing the expression of macrophage proteins mediating actin cytoskeletal rearrangements such as WD repeat-containing protein-1 (WDR1), Alpha actinin-1 (ACTN1), Vinculin (VCL) and Protein disulfide isomerase (P4HB) and intracellular S. Enteritidis growth and replication such as V-type proton ATPase catalytic subunit A (ATPV1A). Interestingly, sodium butyrate increased the expression of infected HTC cell protein involving in bacterial killing such as Vimentin (VIM). In conclusion, sodium butyrate modulates the expression of HTC cell proteins essential for S. Enteritidis invasion.

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Section: Sic Of Sodium Butyrate Against S Enteritidismentioning

confidence: 99%

Section: Plos Onementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Sodium butyrate modulates chicken macrophage proteins essential for Salmonella Enteritidis invasion

et al. 2021

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“…The microbiota, using several biochemical pathways, metabolizes both diet- and host-derived metabolites that then influence various components of the intestinal immune system. For example, the microbiota converts nondigestible fibers to short chain fatty acids ( SCFA ; acetate, propionate, butyrate) that have several anti-inflammatory activities on chicken immune cells both in vitro and in vivo ( Zhou et al, 2014 ; Zou et al, 2019 ; Gupta et al, 2020 ).…”

Section: Factsmentioning

confidence: 99%

Role of diet-microbiota interactions in precision nutrition of the chicken: facts, gaps, and new concepts

Kogut

2022

Poultry Science

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In the intestine, host-derived factors are genetically hardwired and difficult to modulate. However, the intestinal microbiome is more plastic and can be readily modulated by dietary factors. Further, it is becoming more apparent that the microbiome can potentially impact poultry physiology by participating in digestion, the absorption of nutrients, shaping of the mucosal immune response, energy homeostasis, and the synthesis or modulation of several potential bioactive metabolites. These activities are dependent on the quantity and quality of the microbiota alongside its metabolic potential, which are dictated in large part by diet. Thus, diet-induced microbiota alterations may be harnessed to induce changes in host physiology, including disease development and progression. In this regard, the gut microbiome is malleable and renders the gut microbiome a candidate ‘organ’ for the possibility of precision nutrition to induce precision microbiomics—the use of the gut microbiome as a biomarker to predict responsiveness to specific dietary constituents to generate precision diets and interventions for optimal poultry performance and health. However, it is vital to identify the causal relationships and mechanisms by which dietary components and additives affect the gut microbiome which then ultimately influence avian physiology. Further, an improved understanding of the spatial and functional relationships between the different sections of the avian gut and their regional microbiota will provide a better understanding of the role of the diet in regulating the intestinal microbiome.

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“…For example, after exposure to phenolic fungicides or subinhibitory concentrations of triclosan, the frequency of mutations in antibiotic resistance of Salmonella increases (Randall et al, 2004). Pathogens will also be transmitted through certain ways, such as, from animals to humans through food chain (Gupta et al, 2020;Lei et al, 2020). Additionally, bacteria can also acquire exogenous resistance genes through other factors in the environment, such as flies (Zhao et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

The Influencing Factors of Bacterial Resistance Related to Livestock Farm: Sources and Mechanisms

et al. 2021

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Bacterial resistance is a complex scientific issue. To manage this issue, we need to deeply understand the influencing factors and mechanisms. Based on the background of livestock husbandry, this paper reviews the factors that affect the acquisition of bacterial resistance. Meanwhile, the resistance mechanism is also discussed. “Survival of the fittest” is the result of genetic plasticity of bacterial pathogens, which brings about specific response, such as producing adaptive mutation, gaining genetic material or changing gene expression. To a large extent, bacterial populations acquire resistance genes directly caused by the selective pressure of antibiotics. However, mobile resistance genes may be co-selected by other existing substances (such as heavy metals and biocides) without direct selection pressure from antibiotics. This is because the same mobile genetic elements as antibiotic resistance genes can be co-located by the resistance determinants of some of these compounds. Furthermore, environmental factors are a source of resistance gene acquisition. Here, we describe some of the key measures that should be taken to mitigate the risk of antibiotic resistance. We call on the relevant governments or organizations around the world to formulate and improve the monitoring policies of antibiotic resistance, strengthen the supervision, strengthen the international cooperation and exchange, and curb the emergence and spread of drug-resistant strains.

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Sodium Butyrate Reduces Salmonella Enteritidis Infection of Chicken Enterocytes and Expression of Inflammatory Host Genes in vitro

Cited by 25 publications

References 78 publications

Sodium butyrate modulates chicken macrophage proteins essential for Salmonella Enteritidis invasion

Sodium butyrate modulates chicken macrophage proteins essential for Salmonella Enteritidis invasion

Role of diet-microbiota interactions in precision nutrition of the chicken: facts, gaps, and new concepts

The Influencing Factors of Bacterial Resistance Related to Livestock Farm: Sources and Mechanisms

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