Sodium/calcium exchanger in olfactory receptor neurones of Xenopus laevis

Jung, Anita; Lischka, Fritz W.; Engel, Jutta; Schild, Detlev

doi:10.1097/00001756-199409080-00013

Cited by 47 publications

(34 citation statements)

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“…These results suggest, therefore, that OSN knobs use NCX as their primary Ca 2+ i extrusion mechanism in response to stimulus-induced Ca 2+ i elevation (Figure 3E). Furthermore it is consistent with reports from other laboratories [51], [59], [60] suggesting that NCX serves as a major Ca 2+ extrusion pathway for Ca 2+ i transients raised by odorant stimulation. Hence, the slower restitution kinetics in the OMP −/− mice might be indicative of a deficit in NCX activity.…”

Section: Resultssupporting

confidence: 92%

Ca2+ Extrusion by NCX Is Compromised in Olfactory Sensory Neurons of OMP−/− Mice

et al. 2009

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BackgroundThe role of olfactory marker protein (OMP), a hallmark of mature olfactory sensory neurons (OSNs), has been poorly understood since its discovery. The electrophysiological and behavioral phenotypes of OMP knockout mice indicated that OMP influences olfactory signal transduction. However, the mechanism by which this occurs remained unknown.Principal FindingsWe used intact olfactory epithelium obtained from WT and OMP−/− mice to monitor the Ca2+ dynamics induced by the activation of cyclic nucleotide-gated channels, voltage-operated Ca2+ channels, or Ca2+ stores in single dendritic knobs of OSNs. Our data suggested that OMP could act to modulate the Ca2+-homeostasis in these neurons by influencing the activity of the plasma membrane Na+/Ca2+-exchanger (NCX). Immunohistochemistry verifies colocalization of NCX1 and OMP in the cilia and knobs of OSNs. To test the role of NCX activity, we compared the kinetics of Ca2+ elevation by stimulating the reverse mode of NCX in both WT and OMP−/− mice. The resulting Ca2+ responses indicate that OMP facilitates NCX activity and allows rapid Ca2+ extrusion from OSN knobs. To address the mechanism by which OMP influences NCX activity in OSNs we studied protein-peptide interactions in real-time using surface plasmon resonance technology. We demonstrate the direct interaction of the XIP regulatory-peptide of NCX with calmodulin (CaM).ConclusionsSince CaM also binds to the Bex protein, an interacting protein partner of OMP, these observations strongly suggest that OMP can influence CaM efficacy and thus alters NCX activity by a series of protein-protein interactions.

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Section: Resultssupporting

confidence: 92%

Ca2+ Extrusion by NCX Is Compromised in Olfactory Sensory Neurons of OMP−/− Mice

et al. 2009

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“…The cell extends an apical dendrite (D) towards the nasal cavity, terminating in a knob (K) from which a number of sensory cilia (C) protrude into the mucus. From its basal pole, the neurons project a single unbranched axon (A) to the olfactory bulb and probably involves Ca 2+ -ATPases in knobs, dendrites, and cilia [19,37,56,66,70].…”

Section: Odor-induced Electrical Responsesmentioning

confidence: 99%

Molecular physiology of odor detection: current views

Paysan

Breer

2001

Pflügers Arch - Eur J Physiol

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This review outlines current views of the principles and mechanisms underlying olfactory signaling, and presents some thoughts on open questions and future perspectives in this field. We briefly introduce the structure and function of the olfactory system and its sensory neurons, which respond to appropriate odorants with distinct patterns of electrical activity and contribute to the phenomenon of odor coding based on their characteristic receptor repertoire and their specific interconnections with target neurons in the brain. The molecular mechanisms mediating the process of chemo-electrical signal transduction in sensory cells and their functional implications are discussed. As a prerequisite for the high sensitivity and fast kinetics of the transduction process, we propose that the functional elements of the transduction cascades are spatially arranged in multimeric complexes, organized by scaffolding proteins, which anchor the signaling networks to the membrane of olfactory sensory cilia.

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“…8 indicates some of the mechanisms by which Ca 2ϩ can be extruded from ORNs. There is increasing evidence for the presence of a Na ϩ /Ca 2ϩ exchange mechanism in olfactory cilia (Noé et al 1997;Reisert and Matthews 1998) and in the ORN dendrite (Jung et al 1994). A Ca 2ϩ -ATPase has also been isolated from ORNs (Lo et al 1994).…”

Section: Model Of Ca 2ϩ Sigaling In Orns: Ca 2ϩ Regulation Differs Bementioning

confidence: 99%

Amplification of Odor-Induced Ca²⁺ Transients by Store-Operated Ca²⁺ Release and Its Role in Olfactory Signal Transduction

Zufall

Leinders‐Zufall

Greer

2000

Journal of Neurophysiology

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release does not seem to be necessary for generation of an immediate electrophysiological response, nor does it contribute significantly to the Ca 2ϩ transients in the olfactory cilia. Rather, it is important for amplifying the magnitude and duration of Ca 2ϩ transients in the dendrite and soma and is thus necessary for the spread of an odorinduced Ca 2ϩ wave from the cilia to the soma. We show that this amplification process depends on Ca 2ϩ -induced Ca 2ϩ release. The results indicate that stimulation of ORNs with odorants can produce Ca 2ϩ mobilization from intracellular stores without an immediate effect on the receptor potential. Odor-induced, store-dependent Ca 2ϩ mobilization may be part of a feedback pathway by which information is transferred from the distal dendrite of an ORN to its soma.

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Sodium/calcium exchanger in olfactory receptor neurones of Xenopus laevis

Cited by 47 publications

References 0 publications

Ca2+ Extrusion by NCX Is Compromised in Olfactory Sensory Neurons of OMP−/− Mice

Ca2+ Extrusion by NCX Is Compromised in Olfactory Sensory Neurons of OMP−/− Mice

Molecular physiology of odor detection: current views

Amplification of Odor-Induced Ca²⁺ Transients by Store-Operated Ca²⁺ Release and Its Role in Olfactory Signal Transduction

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Sodium/calcium exchanger in olfactory receptor neurones of Xenopus laevis

Cited by 47 publications

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Ca2+ Extrusion by NCX Is Compromised in Olfactory Sensory Neurons of OMP−/− Mice

Ca2+ Extrusion by NCX Is Compromised in Olfactory Sensory Neurons of OMP−/− Mice

Molecular physiology of odor detection: current views

Amplification of Odor-Induced Ca2+ Transients by Store-Operated Ca2+ Release and Its Role in Olfactory Signal Transduction

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Amplification of Odor-Induced Ca²⁺ Transients by Store-Operated Ca²⁺ Release and Its Role in Olfactory Signal Transduction