Sodium caseinate induces mouse granulopoiesis

Domínguez-Meléndez, Vanihamin; Silvestre-Santana, O.; Moreno‐Fierros, Leticia; Aguíñiga-Sánchez, Itzen; Martínez, Ledesma; Marroquín-Segura, Rubén; García‐Hernández, Ana Lilia; Weiss‐Steider, Benny; Marché-Cova, A.; García, Alberto Monroy; L, Mora-García; Santiago‐Osorio, Edelmiro

doi:10.1007/s00011-011-0421-7

Cited by 9 publications

(11 citation statements)

References 44 publications

(60 reference statements)

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“…Interestingly, Domínguez-Melendez et al showed that administration of casein as sodium caseinate in BALB/c mice increased the percentage of myeloid precursors from bone marrow and increased the total number of bone marrow leukocytes resulting from cell proliferation. They also found that casein induced proliferation and activation of granulocytes, and it increased the serum concentration of cytokines such as G-CSF, M-CSF, and GM-CSF [37]. These cytokines are key to the proliferation, differentiation, and activation of granulomonocytic cells, which in turn induce multiple functions within the immune response; since these cytokines regulate inflammation, as is the case for monocyte-macrophages, they are responsible for phagocytosis, which is a crucial event in fungal and bacterial infections; once activated, macrophages are the bridge between activated CD4+ lymphocytes and the adaptive immune response [38].…”

Section: Casein and Hematopoietic Tissuementioning

confidence: 93%

“…On the other hand, the Santiago-Osorio group showed that the IP inoculation of BALB/c mice with sodium caseinate induces in vivo proliferation of the myeloid lineage in the bone marrow. Interestingly, it was observed that the granulocytes had the ability to incorporate BrdU, a thymidine analyte that is incorporated into proliferating cells; further, the cells exhibited a greater ability to phagocytose when compared to cells from mice that had not received treatment [37].…”

Section: The Role Of Caseins In Granulopoiesis and Monocytopoiesismentioning

confidence: 99%

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Caseins as Regulators of Hematopoiesis

Ledesma‐Martínez¹,

Domínguez-Meléndez²,

Aguíñiga-Sánchez³

et al. 2020

Infant Feeding - Breast Versus Formula

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The main physiological role of casein, the main protein component in the milk, is to be a source of amino acids that are required for the growth of the neonate; therefore, casein is considered a highly nutritious protein. Over time, it has been revealed that casein is a protein whose physiological importance reaches levels far superior to the food field, having a wide array of biological activities including antimicrobial activities, facilitating absorption of nutrients, as well as acting as a growth factor and an immune stimulant. Here we analyze how caseins can exert numerous hematopoietic and immunomodulatory actions, their role in granulopoiesis, monocytopoiesis, and lymphopoiesis from the early stages of postnatal development seemingly throughout life, and we wonder if casein could be useful to fight pathogens resistant to antibiotics, inducing a strong immune response in immunosuppressed patients, or even be a prophylactic strategy to prevent infections.

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Section: Casein and Hematopoietic Tissuementioning

confidence: 93%

Section: The Role Of Caseins In Granulopoiesis and Monocytopoiesismentioning

confidence: 99%

Caseins as Regulators of Hematopoiesis

Ledesma‐Martínez¹,

Domínguez-Meléndez²,

Aguíñiga-Sánchez³

et al. 2020

Infant Feeding - Breast Versus Formula

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“…Una vez obtenida la sangre de los ratones, se centrifugó durante 10 minutos a 1.500 rpm para obtener el suero. Posteriormente, se evaluó la concentración de IL-7 en el suero, respetando el procedimiento del kit de ELISA (Mouse Interleukin 7, Immunoassay, R&D Systems, MN, USA) durante las dos horas posteriores al sacrificio (6). La lectura de las placas se realizó a 450 nm en un espectrofotómetro Tecan (Tecan Spectra, Austria).…”

Section: Detección De Citocinasunclassified

“…Nuestro grupo de trabajo demostró que, mediante la inyección de caseinato de sodio, se induce la granulopoyesis en médula ósea, y se incrementan los niveles séricos del factor estimulante de colonias de granulocitos (Granulocyte Colony-Stimulating Factor, G-CSF) y del factor estimulante de colonias de granulocitos y monocitos (GranulocyteMacrophage Colony-Stimulating Factor, GM-CSF) (6). El caseinato de sodio es un agente proinflamatorio capaz de actuar sobre células de origen hematopoyético y, también, incrementa las concentraciones de citocinas con un rol fundamental en la mielopoyesis (7).…”

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El caseinato de sodio incrementa número de linfocitos B en ratones

et al. 2017

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Introducción. El caseinato de sodio, una sal de la caseína utilizada como agente proinflamatorio en ratones, es capaz de inducir granulopoyesis en vivo e incrementar la producción de citocinas esenciales en dicho evento.Objetivo. Evaluar si el caseinato de sodio es capaz de inducir un efecto biológico en células de origen linfoide y la producción de citocinas involucradas con este linaje.Materiales y métodos: Se utilizaron ratones hembra BALB/c de 8 a 12 semanas de edad. Los animales se inyectaron cuatro veces, con intervalos de 48 horas, por vía intraperitoneal con 1 ml de caseinato de sodio (10 % de SFB p/v). La población de linfocitos B y la incorporación de bromodesoxiuridina (BrdU) se analizaron mediante citometría de flujo. La detección de la interleucina 7 se evaluó mediante la técnica de ELISA.Resultados. Tras la inyección por vía intraperitoneal, el número de linfocitos B 220+ provenientes del bazo de ratones tratados con caseinato de sodio aumentó comparados con los que solo recibieron el vehículo como tratamiento (89,01±1,03 Vs. 75,66±2,08), así como la incorporación de BrdU en células B220+ (38,59±4,48 Vs. 11,82±1,04). Se evidenció, asimismo, el incremento en la concentración de la interleucina 7 (IL-7) en el suero de los ratones tratados con caseinato de sodio, comparados con los que solo recibieron el vehículo (62,1±17,5 Vs. 26,9±4,4 pg/ml).Conclusión. El caseinato de sodio fue capaz de aumentar el número de linfocitos B en bazo de ratones, así como inducir la producción de IL-7, citocina clave para la linfopoyesis B.

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“…It is widely known that inoculation of sodium caseinate (CasNa), a salt of casein, the principal milk protein [ 11 ], induces the accumulation of granulocytes and macrophages in the peritoneal cavity of mice and increases the serum levels of cytokines related to HSC mobilization [ 3 , 12 , 13 ]. In this regard, we recently showed that granulocyte activation by CasNa, which resembles stimulation by G-CSF or GM-CSF [ 14 ], increases spleen size, weight, and cellularity [ 15 – 17 ]. This suggests extramedullary hematopoiesis activation, which may involve migration of HSC or HPC [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

Sodium Caseinate (CasNa) Induces Mobilization of Hematopoietic Stem Cells in a BALB/c Mouse Model

Santiago‐Osorio

Ledesma‐Martínez

Aguíñiga-Sánchez

et al. 2015

Med Sci Monit Basic Res

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BackgroundHematopoietic stem cells transplantation has high clinical potential against a wide variety of hematologic, metabolic, and autoimmune diseases and solid tumors. Clinically, hematopoietic stem cells derived from peripheral blood are currently used more than those obtained from sources such as bone marrow. However, mobilizing agents used in the clinic tend to fail in high rates, making the number of mobilized cells insufficient for transplantation. We investigated whether sodium caseinate induces functional mobilization of hematopoietic stem cells into peripheral blood of Balb/c mice.Material/MethodsUsing a mouse model, we administrated sodium caseinate or Plerixafor, a commercial mobilizing agent, and analyzed counts of hematopoietic stem cells in peripheral blood, and then cells were transplanted into lethally irradiated mice to restore hematopoiesis. All assays were performed at least twice.ResultsWe found that sodium caseinate increases the number of mononuclear cells in peripheral blood with the immunophenotype of hematopoietic stem cells (0.2 to 0.5% LSK cells), allowing them to form colonies of various cell lineages in semisolid medium (p<0.05). This effect is similar to that of Plerixafor, and cells transplanted into lethally irradiated mice can restore hematopoiesis at higher percentages than mononuclear cells mobilized by Plerixafor (40% vs. 20%, respectively). Further, a secondary transplant rescued a separate group of irradiated mice from death, proving definitive evidence of hematopoietic reconstitution after hematopoietic stem cells transplantation. Data are presented as mean ± standard deviation. To determine significant differences between the data, one-way ANOVA and the Tukey test were used.ConclusionsCollectively these results show the utility of sodium caseinate as a mobilizer of hematopoietic stem cells and its potential clinical application in transplantation settings.

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Sodium caseinate induces mouse granulopoiesis

Abstract: CasNa induces granulopoiesis with functional granulocytes, suggesting that this molecule could be an innate immune system activator.

Cited by 9 publications

References 44 publications

Caseins as Regulators of Hematopoiesis

Caseins as Regulators of Hematopoiesis

El caseinato de sodio incrementa número de linfocitos B en ratones

Sodium Caseinate (CasNa) Induces Mobilization of Hematopoietic Stem Cells in a BALB/c Mouse Model

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